Sentences with phrase «single cell sorting»

Together with Anna - Maria Divne, I am setting up and establish methods for single cell genomics, from single cell sorting, to whole genome amplification, target screening and sequencing library preparation.Why can you find me on this homepage?

All live cell sorting and single cell sorting was performed on purified PBMCs using either a FACSVantage or ARIAII cell sorter system.

Not exact matches

The single cell organism has an ancestor who probably came to earth frozen ice attached to a meteor that was blown off a distant plant due to some sort of plantary collision between a comet and a planet or two planets, or even a large meter collision... Evolution does not require that you believe to exist, it simply exists.

«It's the same process as for single cell genomics, but for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated Cell Sorticell genomics, but for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated Cell SortiCell Sorting).

They feed on the bottom of the food chain — on single - celled plankton, which larger fish can not eat — and then they become prey for all sorts of upper - level predators like tuna, sea bass and halibut as well as seabirds and marine mammals.

A simpler strategy to confirm the isolation is to repeat the sorting of single cells and to fix and stain them, he adds.

Single cells from this sorted population were then seeded into each well of a 96 - well plate by limiting dilution.

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sorting.

By combining advanced approaches such as cell sorting and single cell analysis, researchers will gain a better understanding of the complex genetic landscape of disease.

While no one can say with certainty what sort of life might be turned up by these experiments, the usual assumption is that it will be microbial, as single - celled life is adaptable to a wide range of environments and requires less energy.

(C) Flow cytometry on cells plated at single cell density in 96 well plates from each sorted fraction.

In a sort of molecular «Where's Waldo,» scientists are often left with no choice but to measure a disease's effects on populations of cells, rather than a single cell.

(B) Percent of sorted single HES3 cells in (A) expressing stem or lineage markers according to level of surface marker expression.

Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein - tagged cells, assessing apoptosis and cell cycle status, the identification / isolation of stem and cancer stem cells (e.g., marker positive, side - population, etc.) and the deposition of single cells.

If human cells are sorted together mouse cells, the data will have heterogenous expression patterns as single cell data does, only with human genes rather than mouse genes.