Further research topics are statistical / computational methods to study complex experimental data (e.g. cellular genealogies) and image analysis procedures (e.g. model - based segmentation algorithms in the context of automatic
single cell tracking).
With respect to the newly emerging field of Bioimage Informatics, our group is specifically interested in the development of automatic
single cell tracking algorithms and their application.
Not exact matches
The technique, called superresolution
single - molecule fluorescence microscopy, recently helped scientists at the University of Manchester in England
track natural killer (NK)
cells, which help destroy cancer and viruses.
After this initial color assignment, the color information was propagated in time using the
tracking information, thus providing a color - coded
single -
cell resolution fate map.
So instead of studying the whole animals, he began isolating
single nerve
cells from the mole rats and investigating them in lab dishes to
track the molecular basis of the rodent's pain insensitivity.
«Such dynamic behaviours were difficult to find using normal techniques, and our findings were made possible by
single - molecule
tracking of new fluorescent ganglioside probes,» said Kenichi Suzuki of the Institute for Integrated
Cell - Material Sciences (WPI - iCeMS) and the paper's co-author.
Malinski's team has developed unique methods and systems of measurements using nanosensors, which are about 1,000 times smaller in diameter than a human hair, to
track the impacts of Vitamin D3 on
single endothelial
cells, a vital regulatory component of the cardiovascular system.
UCLA scientists, in collaboration with teams in China, have used the powerful technology of
single -
cell RNA sequencing to
track the genetic development of a human and a mouse embryo at an unprecedented level of accuracy.
Now, a new approach developed by Dr. Ekaterina Galanzha of the University of Arkansas for Medical Sciences in Little Rock and her colleagues allows for labeling and
tracking of individual circulating cancer
cells throughout the body, thereby helping researchers elucidate the pathways of
single cells from start to finish.
Now, researchers reporting in the
Cell Press journal Chemistry & Biology have developed a new method that allows investigators to label and
track single tumor
cells circulating in the blood.
«Sniffing out stem
cell fates in the nose: Using
single -
cell RNA sequencing and clever statistical analysis to
track stem
cells as they mature.»
By
tracking single B
cell receptor (BCR) molecules we have shown how the cortical cytoskeleton network regulates receptor signalling (Mattila et al. 2013; Treanor et al. 2011; Treanor et al. 2010).
We will use high and super-resolution fluorescence microscopy like total internal reflection fluorescence microscopy (TIRFM) and fluorescence photo - activation localization microscopy (FPALM) to visualize and
track the spatio - temporal dynamics of tethering and SNARE proteins in live and fixed
cells with
single molecule resolution.
Giant deepsea protists (like grape - sized,
single -
celled amoeboid Gromia sphaerica) may have developed more than 1.8 billion years ago, and possible fossilized
tracks from slow rolling around warm seafloors with pseudopodia have been dated to 1.2 billion ago (more).
Nonetheless, building a user - friendly device that allows scientists to
track the activity of a
single gene in all
cell types over the course of a plant's life cycle is a tall order.
These include methods for deconvolution in fluorescence microscopy as applied to cellular morphogenesis, in addition to methods for live -
cell binding measurements using fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and
single molecule
tracking (SMT) as applied to transcription factor regulation.
«On the symmetry of siblings: automated
single -
cell tracking to quantify the behavior of hematopoietic stem
cells in a biomimetic setup» is featured on the February issue cover.
The CellDevo
Track focuses on the study of molecular mechanisms in eukaryotic
cells and their role in tissue formation, with the level of analysis ranging from
single molecules to
single cells, from
cells to tissues, and from tissues to whole organisms.
Scherf N, Franke K, Glauche I, Kurth I, Bornhäuser M, Werner C, Pompe T, Roeder I On the symmetry of siblings: automated
single -
cell tracking to quantify the behavior of hematopoietic stem
cells in a biomimetic setup.
Cell tracking used 2 - dimensional time - lapse microscopy to monitor 70 cells from each MSC population over 24 hours, with video software able to differentiate between slow (Figure A) and fast (Figure B) cell populations (Each colored line represent the track of a single ce
Cell tracking used 2 - dimensional time - lapse microscopy to monitor 70
cells from each MSC population over 24 hours, with video software able to differentiate between slow (Figure A) and fast (Figure B)
cell populations (Each colored line represent the track of a single ce
cell populations (Each colored line represent the
track of a
single cellcell).
In - vivo RGB marking and multicolour
single -
cell tracking in the adult brain.
Title: On the symmetry of siblings: automated
single -
cell tracking to quantify the behavior of hematopoietic stem
cells in a biomimetic setup Authors: Scherf N, Franke K, Glauche I, Kurth I, Bornhauser M, Werner C, Pompe T, Roeder I Date: 2012 Publication Details: Exp Hematol.
The researchers believe noise could play a role, so they combine
single - molecule analysis with time - lapse movies of individual
cells to
track random activity in many different genes as the
cells switch states.
Research enhances
single particle
tracking, allows novel light - emitting diodes, and boosts development of new solar
cells.
Using a unique, automated robotic microscope that can follow a
single cell over days or weeks, the scientists
track the birth, maturation, and death of neurons afflicted with Alzheimer's, Huntington's, and Parkinson's diseases and ALS.
New computational strategies are supported by our
single cell data analysis experts with computational methods deconvolute tissue composition, determine
cell type heterogeneity, identify novel
cell type defining markers or
track transcriptional dynamics.
Biological applications discussed included nanoscale clustering of membrane proteins (particularly during synaptic and T
cell signalling in mammalian
cells), molecular assembly / organisation of protein complexes, and
single particle
tracking.
To achieve these dramatic efficiency gains, Cogenra integrates advanced silicon PV
cells, concentrating optics with
single - axis
tracking and an innovative thermal transfer system in a low - cost and scalable design.