Sentences with phrase «single cell uses»

Every single cell uses water to regulate temperature and maintain proper function.

We have designed a microfluidic device in which we can manipulate, lyse, label, separate, and quantify the protein contents of a single cell using single - molecule fluorescence counting.

Designed by Gabriel Nistor, a scientist in Keirstead's laboratory, the quarter - million dollar system housed at the Sue and Bill Gross Stem Cell Research Center will allow scientists to dissect single cells using lasers, manipulate cells using robotic instruments, and control the climate in the work area.

I work closely with the Single Cell Genomics team as we provide them with the single cells used in their facility.

HES3 cultures were treated with 10 µM Y - 27632 (ROCK inhibitor, Axxora LLC, San Diego, CA) for 1 hour prior to dissociation to single cells using TrypLE ™ (Invitrogen, cat.

High - throughput profiling of protein targets in single cells using multiplexed immunofluorescence and fluorescent bioimaging.

Not exact matches

The goal here is to use «single - cell sequencing to understand how many different cell types there are in the human body, where they reside, and what they do,» as Nature reports.

A school bus was used to transport the arrested to an athletic centre into Ucluelet, where the single holding cell was in no way appropriate for all those arrested.

Adult stem - cells are now being used to grow new body - parts that can be implanted with no risk of rejection, the latter being the single largest cause of organ rejection and subsequent death.

We are all born with instincts, which include the inate knowledge to use our bodies... inate fear responses (fight flight or reason) etc.... all incredible knowledge... yet ALL of that knowledge must come from ONE single cell... The information MUST be stroed electrochemically... but how?

A single - cell embryo is «totipotent» (capable of producing all the cells of the body), and has full use of all the genetic information required to produce all the cell types of the developing body.

Single - celled organisms reproduce by mitosis, whereas multi-cellular organisms reproduce by meiosis, using specialised sex cells, the germ line.

For such a being is, in its body, a multicellular organism, a metazoan — to use the scientific Greek — and the egg is a single cell.

- Chlorella is a single - celled freshwater green plant used as a whole food nutritional supplement.

Alternative protein sources may include the use of by - products currently viewed as waste or the development of new protein sources from plants, lower order animals, or single - cell organisms with a lower environmental impact compared with typical animal - based protein sources.

First, the latest article to predict the demise of email, though in this case the author makes a good point that future computers / cell phones / convergence gadgets will probably combine email, text and IM into a single interface, leaving us free to use the right medium for a given message.

Bracknell, UK, 3 November 2014 — Diversified technology company 3M has announced availability of a unique single - use platform that removes host cell DNA (HcDNA) and proteins (HCP) in various applications within the bioprocessing industry.

3M Delivers Early Purification with New Hybrid Purifiers, Single - use functionalised media provides scalable purification performance in cell culture

Using a method called two - photon microscopy, they routinely measure the activity of hundreds of neurons with single cell resolution.

Researchers from Duke University had previously used CRISPR to correct genetic mutations in cultured cells from Duchenne patients, and other labs had corrected genes in single - cell embryos in a laboratory environment.

Our studies involve the use of a whole - cell patch clamp, an electrophysiological approach to measure the transmembrane current of a single cell, to identify the effects of herbs on the cardiovascular system.

The advantages of MFCs may allow widespread use of diagnostic procedures that are currently too expensive to implement (e.g., prescreening for cancer) while the new capabilities may dramatically improve our ability to understand complex diseases such as cancer (e.g., through single - cell analysis).

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Using frozen samples of Nannochloropsis oculata, a type of single - celled ocean - dwelling algae, Dina Pasini (University of Kent) set out to test the conditions which early life would have had to survive if it did indeed travel through space.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

However, using super-thin electrodes, the Lund group have now shown that no networks are needed: one single cell can learn when it is time to react.

King hypothesizes that proteins that the single - celled ancestors of animals used to interact with the extracellular environment — to capture bacterial prey by binding to their cell surface and to detect chemical signals — were later repurposed to enable cells to stick to and talk to each other.

Using a technique known as single - cell RNA sequencing, the team explored more than 65,000 individual cells that exist under normal or inflammatory conditions, looking for genes that were more active in one state or subpopulation versus another.

Recent reports have found that conventional cell isolation and genome amplification strategies, even using MDA, can yield as many as a million false positive single - nucleotide variants in a human genome, swamping the true positives (1).

The TruePrime Single - Cell Whole Genome Amplification (WGA) kit uses a revolutionary multiple displacement amplification method based on the combination of the recently discovered DNA primase Thermus thermophilus (Tth) PrimPol and the extremely processive, high - fidelity Phi29 DNA polymerase (Phi29 DNAP) to amplify uniformly total genomic DNA of either a single cell or a few Single - Cell Whole Genome Amplification (WGA) kit uses a revolutionary multiple displacement amplification method based on the combination of the recently discovered DNA primase Thermus thermophilus (Tth) PrimPol and the extremely processive, high - fidelity Phi29 DNA polymerase (Phi29 DNAP) to amplify uniformly total genomic DNA of either a single cell or a few ceCell Whole Genome Amplification (WGA) kit uses a revolutionary multiple displacement amplification method based on the combination of the recently discovered DNA primase Thermus thermophilus (Tth) PrimPol and the extremely processive, high - fidelity Phi29 DNA polymerase (Phi29 DNAP) to amplify uniformly total genomic DNA of either a single cell or a few single cell or a few cecell or a few cells.

«This work demonstrates the feasibility of using a single gene editing platform, plus the regenerative power of stem cells to correct genetic mutations and restore dystrophin production for 60 percent of Duchenne patients,» said Pyle, associate professor of microbiology, immunology and molecular genetics and member of the Broad Stem Cell Research Center.

Using the new methods of micromachining, which borrow technology for making computer chips to carve out and build up microscopic structures on silicon wafers, Peter Gammel and his colleagues at Bell Labs / Lucent Technologies reduced three of the critical devices of a cell phone to Lilliputian size that will allow all the components of a phone to be constructed on a single chip.

«Researchers use a single molecule to command stem cells to build new bone.»

Researchers are already using single - cell analysis to identify genetic variations within tumors and tissues, and Langsdorff says the technique is quickly establishing a foothold in microbiology as well.

In order to survive, early single - celled organisms could have used that signal to direct repair, an evolutionary advance that he thinks survives today.

After just a single dose, rat intestinal cells pumped out bacterial lactase for up to 6 months — showing that the gut cells had inserted the gene into their DNA and were using it to manufacture the enzyme.

The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type of single - cell gene - expression analysis.

The paper, by three researchers at the University of Vermont, uses a series of time - lapse videos to show that single cells within a community of bacteria randomly use a cascade of proteins to become more or less antibiotic resistant, even when the community is not threatened by an antibiotic.

Researchers used to think cancer spread when single cells slipped away and set up shop elsewhere.

The work, which appears in the November 27, 2014, edition of Science Express, points to new avenues for producing single - site supported gold catalysts that could produce high - grade hydrogen for cleaner energy use in fuel - cell powered devices, including vehicles.

Tubo et al. used a single - cell adoptive transfer model in mice to answer this question.

The authors used a time - lapse video recording coupled to single - cell molecular analyses to study the process step - by - step and found that fate decision is not a unique programmed event, but the result of a very dynamic process composed of spontaneous fluctuation and selective stabilisation of alternative cellular states.

Using single - cell electrophysiology, Charité - researcher Christian Rosenmund observed that spontaneous vesicle release at the synapse happened twice as often.

Using a single - cell adoptive transfer and spleen biopsy method, we found that in mice, essentially all microbe - specific naïve cells produced memory cells during infection.

Before now, a lot of this epigenetic research had been done in yeast — single cell organisms that also use enzymes to lay chemical tags on histone proteins.

Built into a single cell, the laser might one day be used for light - based therapeutics, perhaps killing cancer cells deep inside the body.

These single - celled organisms use the instructions from the wormwood to produce artemisinic acid, a precursor to the drug.

It's more efficient than previous devices, the researchers say, because its two cells absorb more light than single - layer solar devices, because it uses light from a wider portion of the solar spectrum, and because it incorporates a layer of novel materials between the two cells to reduce energy loss.

To overcome these problems, Min and his team developed a new modality to visualize glucose uptake activity inside single cells based on stimulated Raman scattering (SRS) imaging, and demonstrated its use in live cancer cells, tumor xenograft tissues, primary neurons and mouse brain tissues.

Although both methods find great use in clinical application, they do not have sufficient spatial resolution to visualize glucose uptake down to single cells.