Sentences with phrase «single cells sampled»

Utilize turn - key analysis pipelines and visualization tools for investigating variation within whole genome, exome and single cell samples.

Not exact matches

Acquiring high - definition images of every cancer cell found in a blood sample, making characterization at single - cell resolution possible.

Zhang's group is moving beyond bulk sequencing of tumor samples and is now conducting genomics on single cells.

Using frozen samples of Nannochloropsis oculata, a type of single - celled ocean - dwelling algae, Dina Pasini (University of Kent) set out to test the conditions which early life would have had to survive if it did indeed travel through space.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

Careful sample handling is an essential requirement of many single - cell isolation strategies, a family of methods that has grown exponentially in recent years.

In a recent proof - of - concept experiment, Abate and his colleagues performed single - cell genomic sequencing on a sample of seawater.

He also recommends performing standard bulk - cell sequencing on a portion of the sample, to provide a basis for comparison with the single - cell DNA data.

Barbara Hinney and her colleagues from the Institute for Parasitology at the University of Veterinary Medicine, Vienna, screened 298 faecal samples taken from cats across Austria for single - cell intestinal parasites, so called enteric protozoa.

Tissue engineers have been unable to grow epidermis with the functional barrier needed for drug testing, and have been further limited in producing an in vitro (lab) model for large - scale drug screening by the number of cells that can be grown from a single skin biopsy sample.

This family tree captures just a sampling of single - cell and single - nucleus RNA sequencing technologies that have burst onto the scene over the last eight years.

The limitation of ChIP - seq is that it can not deliver a conclusive picture of transcription factor binding in a single cell because it samples events in a population after the cells have been exposed to various toxic chemicals.

The single - cell technique is better at detecting subtle mosaicism than usual DNA sequencing methods, which sequence many thousands or millions of cells mixed together and read out an average for the sample.

Researchers of KIT and the Center for Nanotechnology (CeNTech), Münster, have now developed a clinical method to reliably detect and isolate single cancer cells in blood samples in cooperation with the University Hospital of Hamburg - Eppendorf (UKE).

Recently, technology had advanced to the point that scientists could analyze single cells in those samples.

«Our circulating tumor cell device can release a tumor cell captured from a blood sample, enabling single cell analysis,» says Liu.

The second high - impact / high - risk grant received will be used to explore a new technology to rapidly detect cancerous cells inside a single blood sample.

Information obtained via single - cell sequencing from cells sampled from each core indicate the likely location of a cancerous tumor (dotted lines encompassing cores 2, 3 and 6).

For the new study, described in the October 23rd issue of Nature Communications, scientists at the Johns Hopkins Kimmel Cancer Center and Dana Farber Cancer Institute in Boston collected tissue samples containing normal cells, ovarian cancers, metastases that had spread elsewhere, and small cancers found in the fallopian tubes, which included single cell layers of cancer called «p53 signatures» and serous tubal intraepithelial carcinoma, or STIC lesions.

Here they photographed organisms from deep - water samples and used technology to sequence the genomes of these single - celled organisms.

The tail allows Stentor to attach itself to the bottom of the body of water, while the mouth samples available food and selects what to eat, an exceptional ability in a single celled organism.

The machine, called a continuous cell concentration device, could make it possible to routinely analyze food or water samples to screen for pathogens within a single work shift at food processing plants.

They diluted the microbial sample such that they could add a single cell per culture well filled with their new media via a technique called dilution - to - extinction.

By seeding elements with an assortment of antibodies, the device could be set up to capture several different cancer cells types using a single blood sample.

Searching for T cells that had infiltrated the tumors, they collected as many as 50 T cell samples from a single tumor; next, the team tested each batch of T cells against other tumor samples from the same patient.

FANTOM5 has generated single molecule CAGE profiles for 574 primary human cell samples, each sequenced to a median depth of ~ 4 million mapped tags per sample.

diSPIM technology enables rapid 3D imaging of samples ranging from single cells to small organisms over the course of hours to days.

«These extensions complement the MIxS suite of metadata standards by defining the key data elements pertinent for describing the sampling and sequencing of single - cell genomes and genomes from metagenomes,» said GSC President and study co-author Lynn Schriml of the Institute of Genome Sciences at University of Maryland School of Medicine.

Currently, we are exploring several environmental samples retrieved from allover the world - ranging from hydrothermal vents in Japan to hot springs in Yellowstone National Park and New Zealand — for the presence of novel archaeal (and bacterial) lineages using cultivation - independent approaches, such as metagenomics and single cell genomics.

Cell Hashing, which enables sample multiplexing, robust multiplet detection and super-loading of scRNA - seq platforms, allowing confident recovery of 4 times as many single cells per experiment.

Since most data to date has been collected from post-mortem samples, the preferential usage of a single major isoform in living cells may be even more prevalent than previously reported.

The program provides accurate, rapid genotyping and chimerism analysis; automatically identifies donor and recipient peaks in post-BMT samples, calculates percent chimerism and quality metrics for single donor or double donor cases, easily appends for longitudinal monitoring post-BMT, and has multi-lineage capabilities for chimerism analysis of T - cells, B - cells, and other cell type populations.

For each sample, single HSCs were isolated and grown into colonies of homogenous cells to obtain enough DNA for exome sequencing.

«If we can pinpoint which genes control the development of each neuron type, we can generate them in the petri dish from a single sample of human skin cells,» said Dr. Huang.

The result is a three - dimensional map of the elements» placement on a sample smaller than a single blood cell.

(B) Shown is a representative plot of viability eFluor780 stained samples (left) and the percentage of live cells (mean ± SD, right) of a single representative experiment, shown in Fig 1D of 3 independent experiments.

This allows for a one - step PCR reaction and pooling of amplicons from several different samples and targets (generated with other LymphoTrack Assays for the Illumina ® MiSeq ® instrument, sold separately), onto one MiSeq ® flow cell allowing for up to 24 samples per target to be analyzed in parallel in a single run.

Each sample tends to hold hundreds of thousands — or even millions — of single - celled organisms.

The Vijayanand lab has developed a number of genomics (transcriptomic, single - cell and epigenomic) and immunological assays to enable research with small numbers of cells and small - volume clinical samples.

In particular, this meeting will focus on addressing the technological challenges and opportunities presented by the Human Cell Atlas in areas including large - scale single - cell RNA sequencing, spatially - resolved methods, single - cell proteomics, epigenomics, and sample handlCell Atlas in areas including large - scale single - cell RNA sequencing, spatially - resolved methods, single - cell proteomics, epigenomics, and sample handlcell RNA sequencing, spatially - resolved methods, single - cell proteomics, epigenomics, and sample handlcell proteomics, epigenomics, and sample handling.

«Sample multiplexing of peripheral immune populations for high throughput single - cell RNA - sequencing»

In particular, this meeting focused on addressing the technological challenges and opportunities presented by the Human Cell Atlas in areas including large - scale single - cell RNA sequencing, spatially - resolved methods, single - cell proteomics, epigenomics, and sample handlCell Atlas in areas including large - scale single - cell RNA sequencing, spatially - resolved methods, single - cell proteomics, epigenomics, and sample handlcell RNA sequencing, spatially - resolved methods, single - cell proteomics, epigenomics, and sample handlcell proteomics, epigenomics, and sample handling.

The sample was scanned through the laser beam and each cell was identified according to its fluorescence (Figure 8B), and was then exposed to a single pair of laser pulses (Figure 8C).

The rapid test is based on a new plastic microfluidic chip where bacteria from a urine sample are trapped and methods for analysing their growth at single - cell level.

By using a common cell culture step, it is possible for the pathogens in a sample to multiply before the test, which allows for detection of a single original cell.

We used a set of well - characterized DNA samples, DNA mixes and single cells to thoroughly control the study.

Furthermore, multiplexing capabilities are highly desirable to obtain comprehensive, single - cell - level co-expression information and to maximize the use of limited biopsied sample material.

The probe contains Martian soil samples from which British exobiologist Hugh Derry (Ariyon Bakare) is able to isolate a single - celled organism, which he aims to bring back to life.

The goal: create samples of mutants of a single - celled green algae (Chlamydomonas reinhardtii) which used one of two possible pathways preferentially.

The team isolated single cells from the samples and sequenced genomes (the complete set of inherited instructions for an organism) of 738.