Sentences with phrase «single gene sequence»

The even greater surprise is the recent discovery that epigenetic signals from the environment can be passed on from one generation to the next, sometimes for several generations, without changing a single gene sequence.

«This Y is encoded by one single gene sequence.

Not exact matches

Here's why: In the late 90s, technology emerged enabling researchers to rapidly sequence RNA and DNA — giving them the ability to isolate and record the activity of single genes.

In the new study, the researchers sequenced the entire genome of mice that had undergone CRISPR gene editing in the team's previous study and looked for all mutations, including those that only altered a single nucleotide.

When the researchers compared the mRNA to a library of DNA sequences taken from the dendrites of neurons by James Eberwine of the University of Pennsylvania Medical Center, they found that it came from a single gene on chromosome X — the human version of which, when mutated, leads to fragile - X syndrome.

Each guide RNA might hit a single gene or multiple genes, depending on the particular guide sequence.

Almost immediately such familial genome sequencing proved its value, uncovering mutations responsible for diseases caused by defects in a single gene.

After gene - typing about 3,000 people, Sturm found that how OCA2 is expressed — and how much pigment a person has — is strongly linked to three single nucleotide polymorphisms (SNPs), or single letter variations, in a DNA sequence near the OCA2 gene.

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

Using a combination of old - fashioned clinical observation and modern biochemical analysis, he has shown that a person's appetite and their eating behavior can be linked to specific genes — and that even a tiny defect such as the absence of a single nucleic acid in a sequence of DNA can lead to runaway weight gain.

Using a technique known as single - cell RNA sequencing, the team explored more than 65,000 individual cells that exist under normal or inflammatory conditions, looking for genes that were more active in one state or subpopulation versus another.

The samples were analyzed by liquid chromatography - mass spectrometry (LC - MS) to characterize the metabolite composition («metabolomics»), and biocrust DNA was extracted for shotgun sequencing to measure single copy gene markers for the dominant microbe species («metagenomics»).

No one is arguing that Lamarck got everything right, but over the past decade it has become increasingly clear that environmental factors, such as diet or stress, can have biological consequences that are transmitted to offspring without a single change to gene sequences taking place.

Cystic fibrosis, achondroplasia, phenylketonuria and Huntington's disease are each caused by simple changes in the sequence of a single gene.

Molecular geneticists dream of having a similarly comprehensive view of networks that control genes: For example, they would like to identify rules explaining how a single DNA sequence can express different proteins, or varying amounts of protein, in different circumstances (see p. 80).

«Single - nucleus RNA sequencing, droplet by droplet: DroNc - Seq, technology that merges single - nucleus RNA sequencing with microfluidics, brings new scale to gene expression studies in complex tissues.&Single - nucleus RNA sequencing, droplet by droplet: DroNc - Seq, technology that merges single - nucleus RNA sequencing with microfluidics, brings new scale to gene expression studies in complex tissues.&single - nucleus RNA sequencing with microfluidics, brings new scale to gene expression studies in complex tissues.»

RNA sequencing of both single and clustered CTCs from breast cancer patients identified several genes expressed at elevated levels in CTC clusters, one of which — a protein called plakoglobin — also was overexpressed in the primary tumors of patients with reduced survival.

Although the gene sequences were not identical to the Mikovits - Ruscetti XMRV gene sequence reported in Science, they were so close Lo believed he had found genetic variants of a single MLV - like virus species that likely included XMRV.

Different versions of genes can vary at single points in their sequence, and these variations are called SNPs.

«By sequencing all of the DNA that codes for mRNA and ultimately, proteins, Dr. Amin and colleagues found a single gene that may account for as much as 4 % of the heritable risk for depression,» said Doctor John Krystal, Editor of Biological Psychiatry.

With the completion of the first phase of the Human Genome Project in 2000, and the advent of sequencing technologies that can detect gene variations such as single nucleotide polymorphisms (SNPs), for the first time scientists have the tools in hand to find the key immune genes and genetic networks that play roles in vaccine response.

The team found that a single polymorphism (a difference in one «letter» of the sequence) in the BDNF gene accounted for significant differences in intelligence among those with similar injuries and comparable intelligence before being injured.

Comparing their gene sequences established that they likely diverged from a single common ancestor between 1902 and 1921, suggesting HIV has been in human populations for at least that long.

With single - cell RNA sequencing, much more gene transcription was detected than before.

Using single cell RNA sequencing analysis, the Cairns lab profiled cells individually, establishing the gene expression profile in human sperm stem cells.

A new technique for sequencing the RNA in a single cell has been revolutionary, Ngai said, allowing researchers to trace which stem cells in a densely packed tissue become specialized, based on the mRNA present in the cell, which indicates which genes are being expressed.

The study used whole - exome sequencing to characterize genetic alterations that occur at the single nucleotide level for all genes in 25 cell lines commonly used as models of bladder cancer.

Single - cell RNA sequencing allows researchers to determine the precise nature of the total gene transcripts, or all of the genes that are actively expressed in a particular cell.

By using faster sequencing machines and targeting only one highly variable part of the rRNA gene, he and his team can sequence 200,000 pieces of DNA from a single liter.

«A single genome does not reveal the large store of genetic diversity in rice,» says Leung, who notes that many important genes are not present in the previously sequenced rice lines.

This study further investigated the impact of nicotine use / smoking status and variation in the mu opioid receptor gene (OPRM1), specifically, an A118G single nucleotide polymorphism (SNP, or DNA sequence variation), on the effects of naltrexone on a range of drinking outcomes.

The team also compared the 7442 genes found as single copies in both the cobra and the python with the same genes in all other land vertebrates sequenced so far.

Through the combination of linkage data and exome sequencing, they have identified a deletion or loss of a single base in the gene encoding STAG3, which results in a prematurely truncated protein without function.

Thus µSCALE empties the contents of a single capillary onto a collector plate, where the DNA of the isolated cell can be sequenced and the gene variant responsible for the protein of interest can be identified.

Silent mutations occur when the change of a single DNA nucleotide within a protein - coding portion of a gene does not affect the sequence of amino acids that make up the gene's protein.

Each point represents one individual donor and is averaged from 25 — 75 sequences, except for the primary response to anthrax from which only 10 VH genes could be cloned from single cells because of the highly limited response.

Other medical sequencing projects will use DNA sequencing to: discover new genes that are involved in common diseases; identify the genes responsible for dozens of relatively rare, single - gene (autosomal Mendelian) diseases; sequence all of the genes on the X chromosome from affected individuals to identify those involved in sex - linked diseases; and survey the range of variants in genes known to contribute to certain common diseases.

We used high - throughput sequencing of 16S rRNA genes to characterize the airborne bacterial contribution of a single person sitting in a sanitized custom experimental climate chamber.

If you took high school biology in the 1990s, you probably learned about the molecular basis for human genetic disorders such as cystic fibrosis (1989), Huntingtons (1993), Duchenne and Becker muscular dystrophy (1987), and a rapidly growing list of single - gene disorders, and the correspondingly rapid growth in clinical diagnostic technology based on DNA sequence information, enabling certain diagnosis, sometimes before the advent of overt symptoms.

Sequencing accelerates the analysis of cancer associated alterations in genome sequence such as insertions and deletions, CNVs, inversions, duplications, translocations and gene fusions, as well as single nucleotide variants.

The gene expression from every sample was amplified in triplicates, with a single volume of 10 µl containing: 3 µl of diluted cNDA with 0.5 µl of primer set (see Table 1 for primer sequences), 1.5 µl double distilled water (DDW) and 5 µl of GoTaq qpcr Master Mix Kit (Promega, USA).

In each case they checked tiny sites of known DNA - sequence variations — «single nucleotide polymorphisms» — that mark common gene variants.

Using gene sequencing, the researchers learned that every member of the family with congenital heart disease had the same mutation in GATA4 — a change in a single letter in the gene.

Insertional mutagenesis with a single P element provides a powerful approach to obtaining mutations in silent genes when carried out in the context of whole genome sequencing.

July 3, 2013 New mechanism for human gene expression discovered In a study that could change the way scientists view the process of protein production in humans, University of Chicago researchers have found a single gene that encodes two separate proteins from the same sequence of messenger RNA.

To avoid these problems, the team used sequence data to create a list of single DNA letter changes, known as SNPs, which can be reliably identified in the gene - rich areas of the genome.

They did their best to filter out genes known to have random monoallelic expression, single - allele expression due to sequence variants, or RNA patterns that might look like monoallelic expression due to technical issues with the sequencing.

Moving from DNA barcoding to metabarcoding perfectly embodies the conceptual transition from single gene to massively parallel genome analysis, introducing students to Next Generation Sequence (NGS) analysis and data science.

Our supermatrix expands upon these previous studies by bringing together sequences from a wide array of molecular studies, supplemented by new data for four genes, to yield a single primate phylogeny with strong support for the monophyly of most primate genera, families, and higher - level taxa (Primates, Strepsirrhini, Lemuriformes, Lorisiformes, Haplorhini, Tarsiiformes, Simiiformes, Catarrhini, Platyrrhini).

Studying a specific type of genetic variation in the DNA sequences of wolves and domestic dogs - called Single Nucleotide Polymorphisms (SNPs)- the scientists identified the transfer of dog gene variants into wolf genomes.