Sentences with phrase «single nucleotide base»
CRISPR / Cas9 and related tools can now be used in new ways, such as changing a single nucleotide base — a single letter in the genetic code — or adding a fluorescent protein to tag a spot in the DNA that scientists want to track.
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The team found that a mutation in a single pair of nucleotides in the gene causes seed coat permeability — that is, a change in one pair out of the approximately 1 billion base pairs that make up the soybean genome.
Then they induced a single, 12 - base strand of DNA from the human p53 gene to build a complementary copy of itself out of the modified nucleotides.
One commercially available alternative is pyrosequencing, which also detects nucleotides as they are added to a single strand of DNA, but it can lead to errors because it is harder to differentiate between a single base and a whole stretch of identical bases next to each other, says Elaine Mardis, a geneticist at Washington University in St Louis, US.
All of these studies were genome - wide association studies (GWAS) based on millions of genetic variants called Single Nucleotide Polymorphisms (SNPs).
For the analyses, Thompson and his colleagues looked for single - letter (nucleotide base) changes in DNA that correspond to the sizes of key brain regions.
The researchers compared the dogs» DNA, looking for sequences that differed by a single base, known as single - nucleotide polymorphisms.
To study natural selection, the team combed the International Haplotype Map for long stretches of DNA flanked by a single nucleotide polymorphism (SNP, or «snip»)-- that is, an altered base, or «letter,» in the genetic alphabet.
They pick out single bases that don't match the equivalent bases in another species — so - called single nucleotide polymorphisms.
Gregory Koblentz, deputy director of the biodefense program at George Mason University in Fairfax, Virginia, speculates that the FBI may have used a recent technique based on the identification of Single - Nucleotide Repeat markers in the anthrax genome that allows for differentiating between variants of the same strain.
The teams then scanned their respective databases for single - nucleotide polymorphisms — regions where the DNA differs by one base — that were associated with being either taller or shorter than average.
Previous genetic studies have examined the association of aspirin, NSAIDs, or both with colorectal cancer according to a limited number of candidate genes or pathways.6 - 10 Thus, to comprehensively identify common genetic markers that characterize individuals who may obtain differential benefit from aspirin and NSAIDs, we conducted a discovery - based, genome - wide analysis of gene × environment interactions between regular use of aspirin, NSAIDs, or both and single - nucleotide polymorphisms (SNPs) in relation to risk of colorectal cancer.
Large - scale resequencing of the first ESTs and subsequent genotyping of single nucleotide polymorphisms in large populations expanded the available molecular marker resources and provided a basis for examining their association with traits of interest (Eckert et al. 2013).
Based on the nucleotide context, the authors suggest that a new mutational mechanism may be at work involving cytosine deamination of single - stranded DNA (presumably during replication).
A genetic score (GS) was calculated on the basis of suggestive single nucleotide
A genetic score (GS) was calculated on the basis of suggestive single nucleotide... polymorphisms (SNPs) found by association analyses.
The term «single - nucleotide polymorphism» (SNP) refers to a single base change in DNA sequence between two individuals.
They may provide as much as three times the amount of base variation as the better - known single nucleotide polylmorphisms (SNPs), where single base pairs vary between individuals without affecting function.
(Left) A single DNA strand (formed by a sugar - phosphate backbone and nucleotide base - pairs).
A single DNA sequence is formed from a chain of four nucleotide bases and if some individuals in a population do not carry the same nucleotide at a specific position in the sequence, the variation is classified as an SNP.
A novel MALDI - TOF based methodology for genotyping single nucleotide polymorphisms.
Genome - based comparison of E. coli from infected patients and their immediate environment indicated low genetic similarity overall between the two, although three clinical - environmental isolate pairs differed by ≤ 5 single nucleotide polymorphisms.
These modifications of the genome range from single - base changes (single - nucleotide variants) to insertions or deletions of a few bases (indels) to chromosomal rearrangements and occur during the whole life, starting from the first division of the embryo.
In addition to the large CNV mutations they had spotted earlier, they also found de novo changes in single base pairs of DNA — known as single - nucleotide variants (SNVs)-- and small de novo insertions or deletions in chromosomes, all of which made the picture even more complex.
We have established two inbred lines that both show a significant overall reduction in genetic diversity based on microsatellite genotyping and genome - wide single nucleotide polymorphism (SNP).
They first looked for individual letters in DNA, called bases, that varied from one genome to the next, identifying about 4 million of these so - called single - nucleotide polymorphisms (SNPs).