Sentences with phrase «sliding microtome»

Brains were cut into 30 µm coronal sections on a sliding microtome (Leica) and the free - floating sections were used for immunostaining.

Sections are cut at 40 µm thick using a freezing sliding microtome.

Brains were fixed with 4 % paraformaldehyde in 100 mM sodium tetraborate, pH 9.5, for 3 h, cryoprotected with 20 % sucrose - potassium - PBS (KPBS), and sectioned into coronal (30 μm) sections using a sliding microtome (Leica Microsystems Inc, Buffalo Grove, IL, USA).

Not exact matches

The control of biological material must have been a bit more lax in the late 1970s (maybe cautiousness inversely correlates with lapel width), because she was somehow able to sneak a piece of my (our) umbilical cord to her lab microtome, where she carved two thin sections and mounted them on glass slides, just for fun.

Slides with microtome sections (10 mm thick) of wild - type and bsl1 - 1 mutant developing inflorescences were deparaffinized and treated with 5 μg / mL proteinase K at 37 °C for 20 min followed by refixation in PFA solution (4 % [w / v] paraformaldehyde, 5 % [v / v] acetic acid, and 50 % [v / v] ethanol) for 10 min.

Remaining prostate tissue was formalin - fixed and paraffin - embedded (FFPE), sectioned with a microtome and placed on microscope slides for FISH analysis (referred to as FFPE sections).