Sentences with phrase «sodium azide in»

The cells were enzymatically removed from the culture dish with Tryple ™ Select (Invitrogen) for 15 min at 37 °C, resuspended in 1 ml FACS buffer I (2 % FBS, 0.01 % sodium azide in PBS), and counted with Neubauer cell counter chambers.

The cells were then washed once with FACS buffer I, once with FACS buffer II (0.01 % sodium azide in PBS), and fixed with 1 % formaldehyde in PBS.

Not exact matches

Shock -, light -, or heat - sensitive materials: Sodium azide — a common preservative in some buffers — comes to mind; it is sensitive to all three.

The brains were then transferred into a solution of 30 % w / v sucrose in PBS that was supplemented with 0.05 % sodium azide and stored at 4 °C until the brains had sunk to the bottom of the containers.

In the case of Clearfield, the industrial compound sodium azide was used to induce mutations.