Sentences with phrase «sodium phosphate buffer»
The cells were resuspended in 1 ml complete media and 4 ml of 30 % OptiPrep (Sigma) in a sodium phosphate buffer and 1 ml of media was carefully layered on top.
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Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and freeze - dried.
The plate was washed with 1.6 mL of binding buffer and proteins eluted with 0.2 mL of elution buffer (20 mM sodium phosphate, 0.4 M imidazole, 0.5 M NaCl, pH 7.4).