Sentences with phrase «somatic cell reprogramming»

These two observations suggest that worker reproduction is influenced by more plastic epigenetic processes than those of queen — worker differentiation — processes, it has been suggested, that could be analogous to somatic cell reprogramming and transdifferentiation [36].

Reduced expression of Paternally Expressed Gene - 3 enhances somatic cell reprogramming through mitochondrial activity perturbation, Scientific Reports.

Buganim Y, Faddah DA, and Jaenisch R, Mechanisms and models of somatic cell reprogramming.

Ilda showed that the Paternally Expressed Gene - 3 (PEG 3) is a novel regulator of somatic cell reprogramming.

Somatic cell reprogramming takes differentiated cells and returns them to a pluripotent state.

(2015) Myc and SAGA rewire an alternative splicing network during early somatic cell reprogramming.

The sequential processes of somatic cell reprogramming to create patient - specific hiPS cells, CRISPR / Cas9 gene editing, and single - cell cloning uniquely enable researchers to study how a specific genetic modification can influence function.

The U.S. patent office's database has more than 200 applications that mention somatic cell reprogramming.

The November 2003 application describes a possible approach to somatic cell reprogramming.

«Given everything we thought we knew about MYC and LIN41 at the time, we couldn't comprehend how these genes were so beneficial in somatic cell reprogramming, but absolutely useless in tumor reprogramming.

Not exact matches

The ultimate goal of our laboratory is to generate ES - like cells directly from somatic cells by nuclear reprogramming... which converts adult cells back into embryonic state.

Subsequent procedures included mammalian somatic cell nuclear transfer, cell fusion, induction of pluripotency by ectopic gene expression, and direct reprogramming.

The approach — reprogramming somatic cells — promises to be a boon for regenerative medicine.

Reprogramming of adult somatic cells into induced pluripotent stem cells (iPSCs) provides a powerful tool for in vitro disease modeling and drug screening.

KLF4 together with other reprogramming transcription factors is used in the lab to force the expression of genes in somatic cells (adult non-germline cells) in the development of iPSCs.

But the favored reprogramming technique, somatic cell nuclear transfer (SCNT), otherwise known as research cloning, is fraught with ethical pitfalls as well as technical difficulties because it entails creating a human embryo by inserting an adult cell nucleus into an ooctye.

A better understand of the mechanism by which somatic cells are reprogrammed into pluripotent cells is critical to ongoing work to understand and to treat disease.

Using a variety of methods to induce somatic cells to become iPSCs, the researchers first found that in the early stages of reprogramming, the transformation was consistently accompanied by an increase in ROS generation.

However, these problems associated with histocompatibility may be solved using autologous donor adult stem cells, therapeutic cloning, stem cell banks or more recently by reprogramming of somatic cells with defined factors (e.g. induced pluripotent stem cells).

It can reprogram human somatic (nonreproductive) cells, rewinding them back to an embryoniclike state.

To sum up, we have seen that somatic cells of various origins, including human, can be lineage reprogrammed into induced neurons.

First discovered a series of small molecules and conditions that can replace reprogramming transcription factors and enhance reprogramming efficiency in generating iPS cells from somatic cells.

It's just what we are telling in a metaphoric way to somatic cells when we reprogram them into neurons.

Factors that drive neuronal specification in the developing CNS, are good candidates for reprogramming other somatic cells into neurons in vitro and perhaps also in vivo.

Human pluripotent stem cells from two sources today, one physiological embryonic stem cells «ES» from the embryo, and the other experimental cells «iPS» induced pluripotency by reprogramming genetic somatic cells.

Review conceptual and technical aspects of direct reprogramming of somatic cells, such as fibroblasts and astrocytes, into induced neurons.

BB: Thank you, Sarah, for this kind introduction and thanks to Abcam for organizing this webinar, and thank you, the audience, for attending this webinar about Direct Reprogramming of Somatic Cells into Induced Neurons.

Disease - specific human pluripotent stem cells, from embryonic origin or derived from reprogramming somatic cells, offer the unique opportunity to have access to a large spectrum of disease - specific cell models.

When the somatic cells are initially reprogrammed with the 4 genes and then allowed to divide, do they go to a blastocyst - like stage in which cells are taken to generate and perpetuate the iPS, or is it something different from that?

With the living evidence of Dolly, and other animals cloned from adult cells, the idea that an adult somatic cell could become a reprogrammed embryonic - like cell regained a spotlight in the scientific community.

In short, there is no blastocyst - like stage — when the somatic cells are reprogrammed, they're immediately cultured as cells in normal adherent culture.

WIKIMEDIA, CSIROAfter human somatic cells are reprogrammed into induced pluripotent stem cells (iPSCs), the resulting cells retain both genetic and epigenetic indicators of the age of the person who donated the somatic - cell progenitors, scientists have found.

It will be important to discover how this procedure achieves reprogramming of the somatic genome and if the cells are truly equivalent to those derived from IVF embryos but it is without question and important step forward.»

The reprogramming of human somatic cells into induced pluripotent stem cells (iPSCs) offers tremendous potential for cell therapy, basic research, disease modeling, and drug development.

In the following year, the direct reprogramming of human somatic cells was accomplished [2], [3].

While studying a rare genetic disease, researchers discovered a signaling pathway linked to the efficiency of reprogramming somatic cells into stem cells.

Reprogramming human somatic cells to pluripotency represents a valuable resource for the development of in vitro based models for human disease and holds tremendous potential for deriving patient - specific pluripotent stem cells.

We also performed qRT - PCR to determine whether the retroviral OCT3 / 4 and KLF4 transgenes were silenced, which has been shown to be a reliable measure for efficient reprogramming of somatic cells into pluripotency [29].

This strategy takes advantage of the cells «somatic memory of origin» and novel reprogramming strategies to make these cells an effective and safe source of cells for the treatment of cartilage defects and osteoarthritis.

The Cas9 / gRNA - modified fibroblasts were subjected to nuclear reprogramming by somatic cell nuclear transfer, resulting in live - born goats carrying single - gene mutation.

In 2012, Dr. Yamanaka was awarded the Nobel Prize in Physiology or Medicine for his discovery that adult somatic cells can be reprogrammed into pluripotent cells.

In the several years since those first reports, new advances in the derivation of hiPSCs from various tissue sources (including those from human patients) and using diverse reprogramming techniques, and in their use as a pluripotent cell source in the induced differentiation of a wide array of somatic cell types, have appeared with almost startling rapidity.

Demonstrated that not a single «master» transcription factor, but rather a combination of factors, are important for reprogramming of cell fate from one somatic lineage back to a pluripotent state.

Somatic cells can be reprogrammed either by nuclear transfer into oocytes or by fusion with embryonic stem (ES) cells.

The susceptibility of a somatic cell to reprogram may depend on how similar its transcriptional profile is to ESCs.

Genetic reprogramming to a pluripotent state of mouse somatic cells was first achieved by ectopic expression of four factors (Oct4, Sox2, Klf4 and c - Myc) using retroviruses [1].

We have shown that cyclic activation of the Wnt / b - catenin signalling pathway enhances cell - fusion - mediated reprogramming of a variety of somatic cells (Lluis et al., Cell Stem Cell 2008, Marucci et cell - fusion - mediated reprogramming of a variety of somatic cells (Lluis et al., Cell Stem Cell 2008, Marucci et Cell Stem Cell 2008, Marucci et Cell 2008, Marucci et al..

While it has been demonstrated previously that more differentiated cells demonstrate a lower reprogramming efficiency [11] and different somatic cell types possess differential reprogramming ability [12], [13], no study to date, to our knowledge, has identified subpopulations of cells within a primary cell population possessing differential reprogramming potential.

The choice of the somatic cell for reprogramming, the reprogramming technology chosen, and the differentiation techniques utilised, all work synergistically towards the production of mature iPSCs - derived chondrocytes which are comparable to patient - derived chondrocytes, in line with Good Manufacturing Practice guidelines for an «off - the - shelf» stem cell product.

This result suggests that further investigation into the identification and isolation of more purified subpopulations from patient - derived somatic cell lines may result in further enhancement of the reprogramming efficiency.