Sentences with phrase «somatic gene»

Some of the concerns raised about somatic gene transfer are related to the possibility that it could inadvertently lead to germline gene transfer.

COSMIC now details the genetics of drug resistance, novel somatic gene mutations which allow a tumour to evade therapeutic cancer drugs.

MyAML is a targeted panel that analyze the coding and non-coding exons of nearly 200 genes along with breakpoint hotspots within 36 of these genes identified in somatic gene fusions.

A number of Invivoscribe LymphoTrack Assays, formatted for use on both the Illumina MiSeq ® and Thermo Fisher Ion PGM ™ platforms, were designed to detect somatic gene rearrangements within the antigen receptor loci.

«Another future goal, however, is to use CRISPR - Cas9 for somatic gene therapy in humans with severe diseases,» Klaus Rajewsky pointed out.

Not exact matches

The survey, described today in a Policy Forum published by Science, randomly presented people with different vignettes that described genome editing being used in germline or somatic cells to either treat disease or enhance a human with, say, a gene linked to higher IQ or eye color.

To answer this question, Murphy tracked which genes were turned on and off over time in the oocytes and somatic cells in C. elegans IGF - 1 and TGF - β mutants, as well as wild - type worms.

«Given everything we thought we knew about MYC and LIN41 at the time, we couldn't comprehend how these genes were so beneficial in somatic cell reprogramming, but absolutely useless in tumor reprogramming.

Subsequent procedures included mammalian somatic cell nuclear transfer, cell fusion, induction of pluripotency by ectopic gene expression, and direct reprogramming.

The function of these genes were involved in molecular maintenance strategies, such as DNA repair, chromosomal maintenance, immune response and programmed cell death The authors argue that the «slower rate of change in these functions is consistent with increased constraint on somatic cell maintenance as would be required in these relatively long - lived and large - bodied mammals, illustrated by the additional large and long - lived species with slower rates in these genes (e.g. double - strand break repair gene XRCC4 is also highly constrained in elephant.»

In fact, somatic ETV6 translocation is the most common gene rearrangement in childhood leukemia.

Porter explains that somatic mutation of the gene ETV6 requires the presence of other «helper» mutations to cause ALL.

Unlike somatic mutation of the gene, it seems as if the germline mutation puts the patient one important step closer to the development of leukemia from the time of birth.

KLF4 together with other reprogramming transcription factors is used in the lab to force the expression of genes in somatic cells (adult non-germline cells) in the development of iPSCs.

They hope that such a discussion would help the public understand the difference between genome editing in a person's somatic cells — cells other than sperm and egg cells — and editing in cells that could pass the changes on to future generations, says Lanphier, who is president and CEO of Sangamo BioSciences in Richmond, California, a company that hopes to use gene - editing technology to treat patients.

«We have developed a sophisticated statistical analysis software that permits us to localize the cancerous genes among the thousands of somatic mutations in the cancer DNA sequence,» answers Sergey Nikolaev.

Plasmablasts induced by pandemic H1N1 infection are highly cross-reactive and have accumulated particularly high levels of variable gene somatic hypermutation.

(D) The average number of somatic hypermutations in the pandemic H1N1 patient plasmablast variable region genes compared with primary IgG plasmablast responses to vaccinia (small pox) or the anthrax vaccine, or after at least 4 boosters with the anthrax vaccine.

Kohli, Manu, et al. «Facile methods for generating human somatic cell gene knockouts using recombinant adeno ‐ associated viruses.»

The IntelliPlexTM Lung Cancer Panel assesses the status of 57 somatic mutations and gene re-arrangements in liquid biopsy samples.

Genome - wide analysis of variance (ANOVA) revealed that germline SNPs / CNVs and somatic CNAs influenced > 39 % of gene expression probes, roughly half acting in cis and half in trans.

Topics for the scientific sessions are: Single cell genomics and gene expression Genetic interactions RNAi and somatic cell genetics Protein - DNA interactions Cancer The meeting also highlights existing opportunities for academic and industrial researchers to access automated cell - based and biochemical technologies based at the Karolinska High Throughput Center; home to one of the most sophisticated, state - of - the - art, ultra-high performance liquid handling and analysis platforms in Europe.

Shen H, Peters A, Baron B, Zhu X, Storb U. Mutation of BCL - 6 gene in normal B cells by the process of somatic hypermutation of Ig genes.

With over 20,000 known protein - coding genes, widespread genetic variation, and the continual accumulation of mutations in somatic tissues, there are plenty of candidates that will meet these criteria by chance alone.

With only a single gene and a toolbox of forward and reverse genetic approaches at hand, C. elegans offers an opportunity to explore the exact role of perlipin - related factors in fat regulation throughout development of many different somatic and germline cells.

The researchers discovered a single letter variant located in TP53, a gene known to play a central role in tumor biology and for accumulating so called somatic mutations, during the development of cancer in patients.

An Open Label, Phase II Study of Neratinib in Patients with Solid Tumors with Somatic Human Epidermal Growth Factor Receptor (EGFR, HER2, HER3) Mutations or EGFR Gene Amplification

BCR / TCR Genic / Regional Probes (372): recognizing transcripts from genes that undergo somatic rearrangement.

Our definition is similar to the European Medicines Agency (EMA) definition of Advanced Therapy Medicinal Product (ATMP): «Medicinal product for human use that is a gene therapy medicinal product, a somatic cell therapy medicinal product or tissue engineered product» (EMA ATMP Reg.

Some ~ 20 % of loci examined exhibited cis - regulatory assocations between somatic CNAs and gene expression.

Andy Peters and Ursula Storb show that the initiation of Ig gene transcription targets Ig gene somatic hypermutation (SHM), by duplicating the variable (V) region promoter upstream of the constant (C) region and showing that a second wave of mutations occurs over the C region.

Peters A, Storb U. Somatic hypermutation of immunoglobulin genes is linked to transcription initiation.

These classifications are based on large amounts of data inclusing clinical data, somatic mutations, gene and alternative transcript expression, or structural DNA modification, and involve high - dimensional statistical machine learning techniques.

The sequential processes of somatic cell reprogramming to create patient - specific hiPS cells, CRISPR / Cas9 gene editing, and single - cell cloning uniquely enable researchers to study how a specific genetic modification can influence function.

But it turns out that a process called somatic cell nuclear transfer (SCNT) can sometimes reactivate genes.

When the somatic cells are initially reprogrammed with the 4 genes and then allowed to divide, do they go to a blastocyst - like stage in which cells are taken to generate and perpetuate the iPS, or is it something different from that?

By comparing the V. carteri germ and somatic cell transcriptomes, Matt and Umen found that the somatic cells had more transcripts from young, lineage - specific genes, whereas the germ cells had more transcripts from ancient genes that are similar to those expressed in stem cells from animals and land plants.

Today's gene therapy alters somatic cells.

He also discovered common somatic mutations in prostate cancer in the human steroid 5a - reductase type II (SRD5A2) gene in 2004.

Cell - Type Transcriptomes of the Multicellular Green Alga Volvox carteri Yield Insights into the Evolutionary Origins of Germ and Somatic Differentiation Programs Gavriel Y. Matt and James G. Umen G3: GENES GENOMES GENETICS 2018 8: 531 - 550; https://doi.org/10.1534/g3.117.300253 http://www.g3journal.org/content/8/2/531

-LSB-...] Back in December, I wrote about two studies that compared global patterns of gene expression between germ cells and somatic cells in Volvox carteri, one by Benjamin Klein, Daniel Wibberg and Armin Hallmann from the University of Bielefeld in Germany and one by Gavriel Matt and Jim Umen from Washington University in St. Louis and the Donald Danforth Plant Science Center, respectively.

Details on the recurrently mutated genes, and structural and functional analysis of somatic rearrangements, are provided as supplementary materials.

One hundred eighty - eight somatic mutations were detected in 141 genes.

At least 10 constructs of each individual HCC sample were sequenced and mutations appearing in more than five constructs were considered as somatic mutations of the p53 gene, not PCR - induced errors.

Instead, they discovered that each V. carteri cell type expresses orthologs of genes from different temporal phases of the light — dark cycle in a related unicellular alga, Chlamydomonas reinhardtii, with somatic cells enriched for expression of dark - phase genes, and gonidial cells enriched for expression of light - phase genes.

Although counterintuitive, this is reminiscent of the way certain pluripotent stem cells in other systems express a greater number of genes — including more ancient genes — than their somatic daughter cells do.

, with somatic cells enriched for expression of dark - phase genes, and gonidial cells enriched for expression of light - phase genes.

The germ cells also expressed more genes overall than the somatic cells did, despite being specialized for reproduction.

Ilda showed that the Paternally Expressed Gene - 3 (PEG 3) is a novel regulator of somatic cell reprogramming.

Members of the TCGA Research Network identified and characterized four glioblastoma subtypes using gene expression, somatic mutation, and copy number data.