Sentences with phrase «sorting cytometry»

All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.

Not exact matches

They have screened combinations of antibodies that bind to specific proteins on the surface of the immature and mature stem cells and that can be used for flow cytometry, a common laboratory technique for sorting cells.

Flow cytometry and sorting data were analyzed with FlowJo software (Treestar) or Cellquest (BD Biosciences), in the case of primary cells.

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

Institutional needs for flow cytometry services were carefully evaluated and a decision was made to transition the operation of the South Campus Flow Cytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's invescytometry services were carefully evaluated and a decision was made to transition the operation of the South Campus Flow Cytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's invesCytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's invesCytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's investigators.

These include cell sorting and analytical flow cytometry to phenotype, quantify and isolate cells and confocal microscopy to capture high resolution images.p

The team has access to state - of - the - art laboratories and animal facilities with BSL2 and BSL3 containment, flow cytometry, cell sorting, histopathology and in vivo imaging (IVIS).

This Core provides flow cytometry and automated cell sorting services.

The Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellCytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellcytometry and cell sorting.

The facility is continuously expanding with the newest software and machinery for both sort and analysis capabilities of up to 18 fluorescent parameters, and is committed to meet all of the flow cytometry needs for the Hubrecht Institute, the UMC Utrecht and the Princess Máxima Center.

Committed to meet all of the flow cytometry needs for the Hubrecht Institute and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellcytometry needs for the Hubrecht Institute and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellCytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellcytometry and cell sorting.

The department has expertise and infrastructure to support bacterial and viral analysis, and the detailed evaluation of cellular and serological immune responses including flow cytometry and cell sorting, Luminex multiplex platforms, real time PCR, microarrays, comprehensive histological staining, immunohistochemistry and in - situ hybridisation.

Flow cytometry was employed to isolate the sorted population of CD81 - negative cells.

Someone who knows a thing or two about the importance of data collection is Michael Andreeff, M.D., Ph.D., professor of Leukemia, who was a pioneer in the develop of flow cytometry, a method for counting and sorting cells that is commonly used in today's clinical trials.

• Flowmetric — Founded by CEO, Ren Capocasale, Flowmetric is a contract research organization focused on providing polychromatic flow cytometry and cell sorting services to bio-pharma and drug discovery companies.

His team applied, among others, techniques of confocal microscopy and cell sorting by flow cytometry which led to the discovery in human muscle biopsies that these myoendothelial cells are located adjacent to the walls of blood vessels.

We acknowledge the OMRF FACS core and the OUHSC Flow Cytometry and Imaging Core for cell sorting and analysis.

The purpose of the flow cytometry core facility is to sort cells for users, and to train facility users on the self - service cytometers and related software for data acquisition and analysis.

(C) Flow cytometry on cells plated at single cell density in 96 well plates from each sorted fraction.

Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein - tagged cells, assessing apoptosis and cell cycle status, the identification / isolation of stem and cancer stem cells (e.g., marker positive, side - population, etc.) and the deposition of single cells.

To further characterize these cells, we sorted the CD45 - expressing cells by immunomagnetic bead separation, and measured cell purity by flow cytometry (Figure 1a — b).

The team at the HZI providing access through the project has access to state - of - the - art laboratories and animal facilities with BSL2 containment, flow cytometry and cell sorting facilities, histopathology and in vivo imaging (IVIS).

Flow cytometry - based sorting was applied to all samples.

I mastered various blotting techniques; performed complex, cell - based assays; and become skilled in the use of flow cytometry (FACS) technology to complete advanced cell sorting, cell counting and biomarker detection.

Tags for this Online Resume: Ecl (Control Lang), Purchasing, Software, ARIA, Injections, Intelligent Call Management, Research, Transcription, Genotyping, Western blot, viral vectors, DNA cloning, RNA extraction, RNA syntehesis, confocal microscopy, cell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan, electrophoresis, Sanger DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells, DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cells