Sentences with phrase «specific cell lines»

In the future this may be useful to generate patient specific cell lines.

Reprogramming adult cells to function like embryonic stem cells is one way researchers hope to create patient - specific cell lines to regenerate tissue or to study specific diseases in the laboratory.

Last March, the foundation opened a privately - funded «safe haven» lab in Manhattan where researchers from Columbia and Harvard are working to create disease - specific cell lines free of federal funding constraints.

In addition, WNV - specific cell lines isolated from older individuals had a higher proportion of T cells that produced IFN - γ, and also a higher proportion that co-produced IFN - γ and IL - 4 (both molecules that promote a strong immune response).

Researchers have already developed dozens of patient - specific cell lines using this new method.

Yesterday, another Harvard team announced that it had generated disease - specific cell lines for nine other genetic diseases, including Parkinson's disease.

In order to localize the whole human proteome on a subcellular level in one specific cell line a third cell line, U-2 OS, is always chosen.

These results clearly indicate that analysis of future xenograft experiments should take careful note of the size of the xenografts as well as how hypoxia influences CSC in the specific cell line used.

Not exact matches

It then travels down the respiratory tract and binds to epithelial cells lining the lung airways via specific molecules on the cell surface.

Furthermore, regulations stipulate that each new cell line generated be independently tested and validated for safety, making it impractical to develop patient - specific cells for therapies.

Another problem is that in its July 2009 Guidelines on Human Stem Cell Research, NIH spelled out specific requirements about embryo donation for newly derived lines, says Pilar Ossorio, a legal scholar who studies research ethics at the University of Wisconsin Law School.

T cells, along with B cells and others, comprise the adaptive arm of the immune system, the body's second line of defense which quickly attacks and «remembers» specific pathogens.

«Cell lines are an immortal, tissue - specific, physiologic test tube,» he says.

Luster's team hopes to further investigate the characteristics of joints that underlie the critical role of C5a in initiating type III hypersensitivity and whether specific molecules expressed on endothelial cells lining joints play a role in transporting C5a and chemokines from the joint space into adjacent blood vessels.

Current thinking regarding type III hypersensitivity is that immune cells within tissues sense the presence of these immune complexes (ICs) through specific receptor molecules and release inflammatory factors called cytokines that activate the endothelial cells lining adjacent blood vessels to promote the recruitment of neutrophils.

McCain would ban scientists from using donor eggs to create disease - specific stem cell lines or chimeric animals to see how human stem cells behave during development.

A study combining tumor cells from patients with breast cancer with a laboratory model of blood vessel lining provides the most compelling evidence so far that a specific trio of cells is required for the spread of breast cancer.

In earlier studies involving animal models and human cancer cell lines, researchers found that breast cancer spreads when three specific cells are in direct contact: an endothelial cell (a type of cell that lines the blood vessels), a perivascular macrophage (a type of immune cell found near blood vessels), and a tumor cell that produces high levels of Mena, a protein that enhances a cancer cell's ability to spread.

In general, lithium alters the way calcium signals are sent and received — and the new cell lines will make it possible to study this effect specifically in bipolar disorder - specific cells.

«But what we've now shown is that RSV has an increased ability to cause airway obstruction because, during an RSV infection, the virus expresses a specific RSV - encoded non-structural protein, or NS2, in epithelial cells, causing the cells to shed from the airway lining and into the airway lumen.

The researchers hope their new cell lines will be a useful resource for studying the cellular and molecular intricacies of Huntington's further, and suggest they may provide a model for examining other diseases of the brain that are specific to humans.

For one, this can influence the ability of bile acids to interact with specific cell receptors in the intestinal lining.

A new technique of direct binding of radioactive DNA was used to screen a complementary DNA expression library from the BJAB cell line in lambda gt11 phage to derive molecular cDNA clones representing a putative B lymphocyte - specific octamer binding protein.

In the different cell types, the loops begin and end at different specific chromosomal locations, so each cell line's genome appears to have a unique population of loops.

Now that they have shown how this defense mechanism works in cultured cells, they hope to study the importance of LINE - 1 jumping and the defenses against it in specific diseases.

A specific lung - targeting molecule is attached to the carrier's surface and the steriod, dexamethasone, an anti-inflammatory, is loaded into the nanogel, which then binds to lung endothelial cells lining the inside wall of the blood vessels where it rapidly delivers the drug.

The possibility of such cells has been debated at least since the 1950s, when researchers found single neurons in the visual cortex of cats and other animals that respond to simple stimuli, such as lines oriented at a certain angle or moving in a specific direction, or light of a particular wavelength.

And, in the future, the potential to insert barcodes in genes and the development of in - line ID kits, that recognize specific strains of cell lines, could make it easier to verify new organisms and their protein products, and track products through supply chains.

Since our collection of cell lines is completely genetically annotated — which means that mutations and expression changes in each line's genes have been documented — we should be able to identify in advance patients who will benefit from specific combinations.

These «pluripotent cell lines» will be especially groundbreaking for studying Gulf War Illness, because they preserve the genetic and possibly epigenetic factors specific to disease susceptibility.

Cell line — specific sensitivities to suppression of protein complexes and biological pathways also emerged, and these could be validated by RNA interference (RNAi) and pharmacologically.

Tissue - specific microvascular endothelial cell lines from H - 2K (b)- tsA58 mice for studies of angiogenesis and metastasis

In this report, we found that CSCs (CD24 − / CD44 + / ESA +) isolated from metastatic breast cell lines are significantly more metastatic than non-CSC populations in an organ - specific manner.

We used this paradigm following the field of neurodegenerative disorders associated to protein misfolding, in which a widely established method for studying propagation of protein aggregates in vitro consists of exposing cell lines to specific protein aggregates.

Cells from each organ - specific line were plated onto two - chamber slides at a density of 1 × 105 cells / chamber and allowed to incubate overnCells from each organ - specific line were plated onto two - chamber slides at a density of 1 × 105 cells / chamber and allowed to incubate overncells / chamber and allowed to incubate overnight.

Transformed cells such as the Jurkat line may show aberrant DNA methylation patterns at specific loci [57], possibly complicating analyses of cytosine methylation and HIV - 1 latency.

Three types of structural variations were visible in the tissue - specific endothelial cell lines (Fig. 2) ⇓.

Because type III cells appear to integrate information from other receptor cells (Tomchik et al., 2007) and use the 5 - HT3 system to transmit information, these previous nerve recording studies may have inhibited transmission of integrated cross-modal information from type III cells, leading to the appearance of strict labeled - line, modality - specific transmission of information.

Durdu, a PhD student in Darren Gilmour's lab at EMBL, found this behaviour in specific groups of cells in the zebrafish: the cells that will develop into the animal's «lateral line», a series of ear - like organs along the fish's flank that allow it to sense changes in water pressure.

The discovery of NGF has also promoted an intensive search for other specific growth factors, leading to the isolation and characterization of a number of proteins with the ability to enhance the growth of different cell lines.

First, a hair follicle contains epithelial cells — a cell type that lines the body's vessels and cavities — as well as a specific kind of adult stem cell called dermal papillae.

AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron - specific class III b - tubulin isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».

«I am excited to embark on this line of research and look forward to the day when patient - specific stem cells are utilized to treat people suffering from debilitating injuries and health conditions.»

These lines would allow scientists to better study conditions ranging from diabetes to Parkinson's disease, and they would provide the basis for potential patient - specific stem cell treatments.

By pairing engineered cell lines containing disease - specific mutations with high throughput screening, we can investigate the role of given mutations in the biology of disease as well as identify potential druggable targets.

In the last decade, PHENOMIN has generated and validated more than 30 cell specific inducible CreERT2 deleter lines (http://www.ics-mci.fr/mousecre).

The National Infrastructure PHENOMIN is pleased to announce the results of its 6th Call: Generation and Characterization of CreERT2 cell specific promoter driven deleter mouse lines.

The most radical news at the conference was the first reported use of CRISPR / Cas9 to generate precise genome edits (changing only a single base pair) in two genes, not only in cell lines in vitro, but also in specific tissues of embryos in ovo.

Highly Synchronized Expression of Lineage - Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines.

My research interests as Ohio Eminent Scholar focused on developing methods for isolating and characterizing extracellular vesicles derived from both cell lines and patient samples, including the specific profiling of microRNA encapsulated in these vesicles.