Sentences with phrase «specific cell types using»

This paper provides a new method for selective inactivation the NMDA receptors in specific cell types using a novel pharmacogenetic methodology.

Not exact matches

Using patient's own tissue and specific combination of reprogramming factors, skin fibroblasts are successfully converted to cell type that... Continue reading Breakthrough Device Looks to Heal Organs

«This is exactly the technology you could use to look for epigenomic changes in specific cell types,» says Martienssen, who is also on the International Human Epigenome Consortium's steering committee.

Bioengineers at the University of California, San Diego have proven that when it comes to guiding stem cells into a specific cell type, the stiffness of the extracellular matrix used to culture them really does matter.

The two - part approach netted a set of mutations seen only at relapse that enabled the team to sift and sort leukemic and normal stem cells using tools developed in the Dick lab a few years ago to zero in on specific cell types fated to relapse.

This is particularly important as it indicates the method can be used in a variety of cell types to create disease models and contribute to the discovery of new disease - specific therapeutics.

To better determine the role of specific chemoattractants in type III hypersensitivity, lead author Yoshishige Miyabe, MD, PhD, a research fellow in Luster's lab, used multiphoton intravital microscopy — an imaging technology pioneered for studies of immune cell movements in living animals by CIID investigator and co-author Thorsten Mempel, MD, PhD — to follow in real time the development of IC - induced arthritis in a mouse model of rheumatoid arthritis.

Human chondrosarcoma HCS - 2 / 8 cells, which are a type of benign bone cancer cells, can be used to investigate how optimization of culture conditions could improve the synthesis of cartilage - specific molecules.»

Some cell types were not killed by treatment with PAL, suggesting it could be used as a specific tumor therapy.

«Use of induced pluripotent stem cell (iPSC) technology» — which involves taking skin cells from patients and reprogramming them into embryonic - like stem cells capable of turning into other specific cell types relevant for studying a particular disease — «makes it possible to model dementias that affect people later in life,» says senior study author Catherine Verfaillie of KU Leuven.

Techniques enabling the isolation of specific cell populations have advanced our ability to identify and characterize niche cell types which may be used to replace or engineer cells / tissue.

Rutgers University scientists have discovered the three - dimensional structure of a gene - specific transcription activation complex, providing the first structural and mechanistic description of the process cells use to turn on, or activate, specific genes in response to changes in cell type, developmental state and environment.

The technique, named cell type specific labeling using amino acid precursors (CTAP), exploits the inability of vertebrate cells to synthesize essential amino acids normally required for growth and homeostasis.

As Notch appears to use cell type - specific machinery to regulate Myc, it may be possible to target the Notch - Myc signaling path in a way that does not disrupt this path in other cell types.

Solt notes that the study strongly suggests that Th17 cells have a pathological role in the development of type 1 diabetes and use of ROR - specific synthetic compounds targeting this cell type may have potential as a preventative therapy for type 1 diabetes.

Because type III cells appear to integrate information from other receptor cells (Tomchik et al., 2007) and use the 5 - HT3 system to transmit information, these previous nerve recording studies may have inhibited transmission of integrated cross-modal information from type III cells, leading to the appearance of strict labeled - line, modality - specific transmission of information.

Using specific, known types of cells allowed the researchers to see where the developing brain is most vulnerable, Song says.

«Since MCR works by targeting specific DNA sequences, in cases where diseased cells have altered DNA as in HIV - infected individuals or some types of cancer, MCR - based methods should be able to distinguish diseased from healthy cells and then be used to selectively either destroy or modify the diseased cells.»

In this edition, we're going to take a look at a very interesting tool that can be used for creating (excuse the pun) specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and / or tissue - specific cell types: Cre - lox recombination.

Following the delineation of some of the basic principles behind bud formation, the researchers then whether they could extend this strategy beyond the liver, and create other organ buds using tissue specific cell types.

Ng2 - Cre is known to be expressed in the male germline, which needs to be considered in using Ng2 - Cre mice for cell type - specific conditional mutagenesis experiments, but the germline expression of Pdgfrb - Cre in both sexes limits the use of Pdgfrb - Cre as deleter for pericyte - related experiments.

By: Sadhana Agarwal, Katherine L. Holton, Robert Lanza Differentiation of human embryonic stem cells (hESCs) to specific functional cell types can be achieved using methods that mimic in vivo embryonic developmental programs.

He has developed novel cellular models of neurological diseases (schizophrenia, MS, autism, Alzheimer's, Parkinson's) using human induced pluripotent stem cells (iPSCs) derived from donor patients and differentiated to specific neuronal cell types.

The team currently is exploring the cellular and molecular mechanisms underlying ischemic preconditioning using mice in which HIF - 1 activity has been knocked out in specific cell types within the heart.

Specific emphasis will be given to various microfluidic designs targeted for use with different cell types.

The collection of protocols includes the isolation and maintenance of stem cells from various species using «conventional» and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell - specific techniques and approaches to tissue engineering using stem cell derivatives.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

The system, originally developed in 2007 and refined by Wall and Callaway for targeting specific cell types in 2010, uses a modified version of the rabies virus to «infect» a brain region, which in turn targets neurons that are connected to it.

Ultimately, we use all this information to establish innovative culture techniques for generating unlimited neural stem cell sources for the derivation of specific types of clinically relevant neuronal or glial cells, for their use in disease modelling and drug discovery platforms, and towards refining their efficacy for future cell replacement - based therapy.

We have recently developed the use of site - specific nucleases to genetically engineer human pluripotent stem cells, which can be maintained indefinitely and differentiated into any cell type of interest.

One reason why we know little about this process of telomerase silencing is because specific telomerase regulation naturally occurs only in human cells but not in mouse cells, a widely used model organism where telomerase is active in all cell types.

September 1, 1997 Targeting gene therapy to specific cells Researchers at the University of Chicago Medical Center have developed the first practical method to restrict the activity of genes used for gene therapy to a specific cell type.

We are using biochemistry and genetics to identify the proteins that enable neurite - specific neuropeptide localization and using cell - type - specific genetic manipulations to disable that machinery and thereby probe the behavioral function of extra-synaptic peptide signaling in specific circuits.

Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types to do more than just label cells.

Using this cell line we show that apparently undifferentiated ES cell cultures consist of at least three cell types defined by this lineage - specific low - level transcription and the expression of the ES cell markers Oct4 and Nanog.

Making use of cell and animal models, my laboratory group addresses some of the fundamental questions of how ethanol exposure injures the liver, with a goal ofidentifying the individual targets of ethanol - induced injury and the specific responses of the key cell types within the liver, as well as the integrated, organismal response to this ethanol - induced injury.

Using both wild - type and immunocompromised mice, we hope to elucidate the role of dengue - specific CD8 + and CD4 + T - cells against Zika.

Using red blood cells modified to carry disease - specific antigens, a team of scientists from Whitehead Institute and Boston Children's Hospital have prevented and alleviated two autoimmune diseases — multiple sclerosis (MS) and type 1 diabetes — in early stage mouse models.

We use the Cre - loxP recombination system to generate mice with cell type - specific inactivation of the IL - 10 gene in order to identify cellular sources of the cytokine that are relevant in various situations of immune challenge.

CReM's research using induced pluripotent stem cells (iPSCs), which self - renew indefinitely as undifferentiated cells that become specific adult cell types, has helped create an inexhaustible source of disease - or patient - specific stem cells.

Since joining Gladstone, McDevitt's lab has created a new method to generate three - dimensional clusters of specific types of cells that can be used to form and maintain tissue constructs.

Using mouse genetics in combination with novel sequencing technologies our lab is able to identify key cell types or candidate factors important for specific remodeling and repair processes of the heart.

I produce and modify these materials to use them in photovoltaics, such as specific types of solar cells.

While drugs can be used to quickly block NMDA receptors throughout the brain, it is hard to target drugs to a specific cell type.

Finally, the strategy applied here for selective delivery of MK801 can be extended to other small molecules for use in brain tissue (Tian et al., 2012), allowing for an approach that combines chemistry, molecular genetics, and neurobiology to dissect cell signaling pathways in specific cell types in the central nervous system.

a) In the subsection «Cell type - specific NMDA - R pharmacology in cocaine - induced synaptic plasticity» we now more clearly introduce the Zweifel and Engblom studies as well as the differences between them: both of which used genetic ablation of Grin1 over 1 week but showed different results with respect to DA neuron synaptic potentiation, which was due to methodological challenges with AMP - R compensation.

I left off in part 1 emphasizing that the energy shortage in the brain is specific to glucose, and proposing that it would be possible to nourish these starving cells if there were some type of alternative fuel they could use.

One option is to use a chemical exfoliator that contains specific types of acid that dissolve dead skin cells.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)

Acting head of Immigrations and Customs Enforcement Thomas Homan told Sen. Ron Wyden the agency does not use cell - site simulators — a type of surveillance gear often referred to as a «Stingray» that can track down a specific mobile device by emulating cell phone towers — to locate undocumented immigrants.