Sentences with phrase «specific for human cell»

Cells were stained with antibodies specific for human cell surface markers: CD326 / ESA - FITC, CD24 - PE, and CD44 - PE - Cy7 (BD Pharmingen, San Jose, CA, USA).

Not exact matches

(1) The simpler depends upon fewer specific conditions and has fewer, less demanding needs; human life, for example, depends upon so many more factors than single - celled marine life does.

Human milk is a bodily fluid which, apart from being an excellent nutritional source for the growing infant, also contains a variety of immune components such as antibodies, growth factors, cytokines, antimicrobial compounds, and specific immune cells [9].

«This is exactly the technology you could use to look for epigenomic changes in specific cell types,» says Martienssen, who is also on the International Human Epigenome Consortium's steering committee.

Strategies that boost the cell's quality control programs, rather than disarm specific pathologic proteins, have looked promising in lab animals that serve as models for human neurodegenerative disorders including Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis (ALS) and frontotemporal dementia.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

Another problem is that in its July 2009 Guidelines on Human Stem Cell Research, NIH spelled out specific requirements about embryo donation for newly derived lines, says Pilar Ossorio, a legal scholar who studies research ethics at the University of Wisconsin Law School.

In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagen.

In a decades - long game of hide and seek, scientists from Sydney's Westmead Institute for Medical Research have confirmed for the very first time the specific immune memory T - cells where infectious HIV «hides» in the human body to evade detection by the immune system.

«Our study shows, for the first time, in human skin that with increasing age there is a specific decrease in the activity of a key metabolic enzyme found in the batteries of the skin cells.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

The researchers hope their new cell lines will be a useful resource for studying the cellular and molecular intricacies of Huntington's further, and suggest they may provide a model for examining other diseases of the brain that are specific to humans.

«If you're looking for very specific molecular targets or pathways in the brain, and how drugs might act on them, the difference between human cells and mouse cells is significant.»

This was done using shRNA technology specific for BRCA1 in human myotubes (skeletal muscle fiber cells).

Researchers at Karolinska Institutet have identified cell surface markers specific for the very earliest stem cells in the human embryo.

They found that human blood samples contained T cells specific for all 20 versions of the virus.

Although this human Gut Chip recreated the villus epithelium of normal intestine and enabled new insights into how flow and cyclic peristalsis affects intestinal differentiation and function, it could not be used to study processes that relied on normal intestinal cells from individual donors, which, for example, is crucial for studying patient - specific responses for personalized medicine.

Using cells from mice and human livers, Toronto General Hospital Research Institute researchers demonstrated for the first time how under specific conditions, such as obesity, liver CD8 + T cells, white blood cells which play an important role in the control of viral infections, become highly activated and inflammatory, reprogramming themselves into disease - driving cells.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

The Mayo Clinic has received $ 3 million from the Glenn Foundation for Medical Research to study how the presence of a specific sort of human cell affects aging.

«We need to develop specific tools to study chemical toxicity in human reproductive cells; this will allow us to accurately assess safety thresholds for different compounds, and re-evaluate the acceptible daily intake levels to protect human health for some of them» said Professor Habert.

In this study we describe the design and pre-clinical evaluation of a CXCR4 - specific ZFN pair (X4 - ZFNs) that specifically and efficiently disrupts cxcr4, rendering human CD4 + T cells permanently resistant to HIV - 1 strains that require CXCR4 for infection.

The researchers» strategy — generating disease - specific nerve cells, identifying a causative gene for developmental defects, validating the gene - specific defect in animal models, and then investigating interactions with other genes both in animal models and in humans — represents a promising new approach for understanding the mechanisms underlying some of the most intractable psychiatric illnesses.

However, Takebe's liver bud has the advantage of being grown from iPS cells, rather than, for example, the primary human hepatocytes used in Bhatia's graft, which could make it useful in modelling rare diseases or examining the specific genetic backgrounds of the iPS cell donors.

By combining SNP - targeting allele - specific silencing and gain - of - function approaches, we showed that a 46 - glutamine expansion in human HTT was sufficient for a dominant - negative effect on spindle orientation and changes in the distribution within the spindle pole and the cell cortex of dynein, p150Glued and NuMA in neural cells.

PERSON 2 says it is wrong to create human embryos for the specific purpose of destroying them for their stem cells.

Thus, neural derivatives of disease - specific human pluripotent stem cells constitute a relevant biological resource for exploring the impact of adult - onset HD mutations of the HTT gene on the division of neural progenitors, with potential applications in HD drug discovery targeting HTT - dynein - p150Glued complex interactions.

«This data allows classification of all human protein - coding genes into those coding for house - hold functions (present in all cells) and those that are tissue - specific genes with highly specialized expression in particular organs and tissues, such as kidney, liver, brain, heart, pancreas.

They then inserted the spatially oriented human stem cells (human rsPSCs) into specific regions of partially dissected mouse embryos and cultured them in a dish for 36 hours.

In an application for a prestigious «Pioneer Award» from NIH this year, he proposed injecting human pluripotent stem cells into pig embryos whose genes for specific organs had been knocked out.

Lloyd Old, Thierry Boon, and colleagues develop the TNF release assay for mouse systems in which release of TNF by T cells could be used to assess specific T cell recognition, facilitating the cloning of human tumor antigens.

Asialoglycoprotein receptor 1 is a specific cell - surface marker for isolating hepatocytes derived from human pluripotent stem cells.

«Once we understand how these drugs impact specific cells and tissues we'll be in a position to design clinical trials for humans,» said Kennedy.

Now researchers at UC San Francisco have taken the first step toward a comprehensive atlas of gene expression in cells across the developing human brain, making available new insights into how specific cells and gene networks contribute to building this most complex of organs, and serving as a resource for researchers around the world to study the interplay between these genetic programs and neurodevelopmental disorders such as autism, intellectual disability and schizophrenia.

Each cell - based treatment approach is presented as a potential therapy for a specific disease or a disease category (e.g.: cardiovascular diseases, metabolic diseases, etc.), studied in animal models or in humans.

We developed and implemented such a nomenclature for the European human pluripotent stem cell registry (hPSCreg), which needs to: 1) unambiguously identify a registered cell line; 2) allow tracing of subclones of a particular line; and 3) enable the assignment of different lines to a specific donor origin.

The specific vocation of I - Stem is to explore all the therapeutic potential of human pluripotent stem cells for applications in patients affected by rare diseases of genetic origin.

Other groups are using human embryonic stem cells, and others are exploring RPE - specific stem cells that can be grown from the adult RPE, for example, from eyes donated to eye banks.

However, the further development this type of therapy requires a reliable source for the large amounts of homogenous functional patient - specific NK cells needed and this has led to the generation of NK cells from human induced pluripotent stem cells (iPSCs)[3].

ENCODE has laid a foundation for these kinds of studies by examining more than 140 of the hundreds of cell types found in the human body and identifying many of the cell type - specific control elements.

Their method, published ahead of print in the Oct. 17, 2008 online edition of Nature Biotechnology, not only provides a practical and simple alternative for the generation of patient - and disease - specific stem cells, which had been hampered by the low efficiency of the reprogramming process, but also spares patients invasive procedures to collect suitable starting material, since the process only requires a single human hair.

The researchers are now looking to develop a specific delivery method for the synthetic cells that can be tested in human subjects.

Reprogramming human somatic cells to pluripotency represents a valuable resource for the development of in vitro based models for human disease and holds tremendous potential for deriving patient - specific pluripotent stem cells.

In sum, human NSCs represent an invaluable source of cells to investigate human iPS induction, and also represent a potential source of cells for deriving patient - specific pluripotent stem cells for modeling human disease.

Our objective is to generate a resource that facilitates research into human immune - related diseases by creating a comprehensive database of cell type - and allele - specific epigenomic and transcriptomic data for the immunology community.

This may be relevant for understanding the evolution of the large human CMV - specific CD8 + T cell populations seen in humans.

In this study, we show that in elderly individuals seropositivity for human CMV leads to the development of oligoclonal populations of CMV - specific CTL that can constitute up to one - quarter of the total CD8 T cell population.

His laboratory recently demonstrated a novel use of a specific gene - targeting technique for human embryonic stem cells.

This effect on cell survival was not endothelial cell - specific, since IC50 values for kahweol treatment of several human tumoral cell lines were similar to those obtained for HUVEC (results not shown).

Using gene - targeted mice and human cells that lack specific components of the immune system, a better understanding of the immune response to DENV and ZIKV is critical for developing much - needed vaccines and antivirals.