Sentences with phrase «specific nucleases»

We have recently developed the use of site - specific nucleases to genetically engineer human pluripotent stem cells, which can be maintained indefinitely and differentiated into any cell type of interest.

software for analysis of PGM sequencing of DNA targeted with artificial sequence - specific nucleases

Through careful mapping of the ribose requirements of Cas9, we develop hybrid versions possessing minimal RNA residues, which are sufficient to direct specific nuclease activity in vitro and in vivo with reduced off - target activity.

Not exact matches

These techniques use enzymes called nucleases to snip DNA at specific points and then delete or rewrite the genetic information at those locations.

Conventional CRISPR uses a guide RNA (gRNA) coupled with an enzyme known as a nuclease, most commonly Cas9, that together attach to a specific stretch of DNA bases; the nuclease then snips the double helix.

In 2009, researchers at Dow AgroSciences in Indianapolis, Indiana, and Sangamo BioSciences in Richmond, California, announced that they had used enzymes called zinc - finger nucleases to insert a gene for herbicide resistance at a specific site in the maize genome (V. K. Shukla et al..

What's more, say the researchers, the cancer - causing effects of off - target deletions mistakenly created by the V (D) J enzyme need to be considered in designing site - specific enzymes for genome modification such as zinc - finger nucleases, TALENS, or CRISPRs.

In it, they sound the alarm about new genome - editing techniques known as CRISPR and zinc - finger nucleases that make it much easier for scientists to delete, add, or change specific genes.

As an approach to inactivating CCR5, we introduced CCR5 - specific zinc - finger nucleases into human CD4 + T cells prior to adoptive transfer, but the need to protect cells from virus strains that use CXCR4 (X4) in place of or in addition to CCR5 (R5X4) remains.

To circumvent this, a CCR5 specific zinc - finger nuclease pair (R5 - ZFNs) has been developed [23].

Citation: Wilen CB, Wang J, Tilton JC, Miller JC, Kim KA, Rebar EJ, et al. (2011) Engineering HIV - Resistant Human CD4 + T Cells with CXCR4 - Specific Zinc - Finger Nucleases.

Science magazine heralded these transcription activator - like effector nuclease proteins, known as TALENS, as a major scientific breakthrough in 2012, nicknaming them «genomic cruise missiles» for their ability to allow researchers to target specific locations with great efficiency.

Each assay (15 µL total volue) contained nuclease free water (5 - 10 µL), cDNA template (2 ng / µL), and gene - specific primers (4.5 pm / µL).

Programmable nucleases, ZFN, TALEN and RGENs enable gene knockout in cultured cells and organisms by producing site - specific DNA double - strand breaks, whose repair via error - prone non-homologous end joining (NHEJ) or microhomology - mediated end joining (MMEJ) gives rise to frameshift mutations.

Speakers will discuss applications of patient - specific and disease - specific stem cell lines, including gene repair (via tools such as the Cas9 nuclease), disease modeling, drug screening, and regenerative medicine.