Sentences with phrase «standard curve»

Relative quantification of original input RNA amounts were calculated by comparison to standard curves using purified PCR product as a template for the mRNAs of interest and were normalized to the amount of acidic ribosomal phosphoprotein P0 mRNA [24,46].

The sensitivity and reproducibility of real - time PCR for each target gene were determined by spiking normal tissue samples with known numbers of spirochetes to establish standard curves.

It was used as a standard for many years at NOAA, and we now add it to our other more advanced Standard Curves and Combined methods, allowing direct comparisons.

The Samsung Galaxy S9 includes the company's now standard curved glass panels on the front and back, with an aluminum frame in between.

One Low - and one High - Heat NDM sample (250 grams each) are only used for the purposes of plotting standard curves (usually 12 curves in one set) in determining the undenatured whey protein nitrogen (WPN) by the modified Harland - Ashworth Method.One Low - and one High - Heat NDM sample are only used for the purposes of plotting standard curves in determining the undenatured whey protein nitrogen (WPN) by the modified Harland - Ashworth Method.

Analyte concentrations are calculated using external standard calibration with a standard curve prepared in a pre-fortified control matrix which has been carried through the extraction procedure.

The data is a series of spectrophotometric plate readings, which allows the researcher to quantify levels of an antigen of interest upon comparison to a standard curve.

When they performed the same experiments on 10 - day - old mutant flies and compared the results with the standard curve, they found that the flies were «older» than their chronological age.

This allowed the researchers to use advanced analysis techniques to search for the genes that seemed to be sensitive to aging, and create a standard curve, or timeline, that described the way they changed.

Insulin concentrations were derived from the standard curve using linear regression.

For each sample type, a standard curve for the TMAdV PCR assay was calculated from 3 PCR replicates at each dilution of nucleic acid extracted from the spiked matrix (data not shown).

Sample absorbances were read at a wavelength of 562 nm with a spectrophotometer and compared to a standard curve.

Quantification was performed in comparison to a RNA - standard of 20 ng / ml -1000 ng / ml RNA supplied with the kit and a standard curve was established.

Curve fitting software was used to generate the standard curve where a four - parameter algorithm provided the best standard curve fit.

The concentrations of the samples were calculated from the standard curve and multiplied by the dilution factor.

(A) Standard curve for Zika virus RNA transcript dilution series showing the threshold cycles Ct plotted against the log10 of Zika RNA transcripts and (B) Standard curve obtained with 10-fold serial dilutions of Zika virus.

The average Ct score for the pre-diluted DNA Standards are plotted against log10 to generate a standard curve, which can then be used to calculate the concentration (nM) of the PCR amplicons derived from sample DNA.

The amplification efficiency (E) of each assay was calculated from the slope of a standard curve generated on a 10-fold dilution in triplicate, using the formula E = 10 -LRB--1 / slope)-1.

The progranulin content was calculated using a standard curve of recombinant progranulin from the same plate, and was normalized to the amount of protein loaded per well.

The absorption of the solution was measured at 620 nm and compared with those that were obtained from a standard curve to determine the amount of sugars on the Ag - NP surface.

Works with all standard curved and flat tile roofs, and all standard rail - based racking systems.

Blackbody just means something radiates in a standard curve across the frequency spectrum according to its temperature.