Sentences with phrase «stem cell markers»

Furthermore, they expressed a whole host of stem cell markers.

Mouse iPSCs demonstrate important characteristics of pluripotent stem cells, including expressing stem cell markers, forming tumors containing cells from all three germ layers, and being able to contribute to many different tissues when injected into mouse embryos at a very early stage in development.

In vitro cell motility as a potential mesenchymal stem cell marker for multipotency.

Clearly, the iPSC and hESC lines were globally more similar to each other than to the NSC line (Fig. 4A), and combined with manual inspection of the gene expression of several known pluripotent (Oct4, LIN28, Sox2 and Nanog) and neural stem cell markers (Sox2, Nestin and Musashi2) as measured on the arrays, we concluded that the reprogramming was successful (Fig. 4A).

The ΔN isoforms of p63 may also serve as stem cell markers in a variety of epithelial cell types, including endometrium [30], cervix [31], breast [32], prostate [33], and in stratified squamous epithelium [21,34].

Review of «In Vitro Cell Motility as a Potential Mesenchymal Stem Cell Marker for Multipotency» from Stem Cells TM by Stuart P. Atkinson

In this project, we identified CD90 as a putative cancer stem cell marker in pancreatic endocrine cancer.

Under these culture conditions, which provide for stem cell maintenance and inhibition of differentiation, as stem cell marker expression begins to drop, cells switch on the expression of lineage specific transcription factors at the RNA level.

The GFP+HPC s expressed a whole range of leukocyte and stem cell markers including CD45, CD11b, CD31; relatively low levels of MHC class I; and no class II antigens, as we recently reported [17].

Stem cells in mammary tissue have been well characterised by using the mammary stem cell marker, cytokeratin (CK) 5 and the mature epithelial markers CK14,...

The investigators then confirmed that these four factors and their corresponding regulatory elements — the DNA segments to which transcription factors bind — were active in from 2 to 7 percent of human glioblastoma cells, cells that also expressed a known stem cell marker.

Coxsackie B virus (red) infects cells positive for the heart stem cell marker Sca - 1 (green).

Further research uncovered a broad spectrum of cell surface stem cell markers (e.g., CD133, CD44, and CD24) that allow the identification of CSCs in human solid tumors, including brain, breast, prostate, pancreas, liver, ovary, skin, colon cancers, and melanoma (3 - 6)(Figure 1 based on 7).

This gave a mixed population of cells with 20 % expressing CD34, a hematopoietic stem cell marker, with some co-expression of endothelial cell markers (CD31, Flk1, and VE - cadherin).

With this reporter we split ES cell cultures into two fractions that both express certain stem cell markers but only one of which expresses low levels of an endodermal marker gene.

Expression of four stem cell marker genes normalized to cyclophilin in nine single cell equivalents prepared from a pool of nine lysed HES3 cells.

They found that ONC201 alters the gene expression of cancer stem cell markers and signaling pathways prior to killing the tumor cells, providing pharmacodynamic biomarkers of response.

Human iPSCs also express stem cell markers and are capable of generating cells characteristic of all three germ layers.

Corresponding to upregulated TRF2 expression at 80μM, stem cell markers like CD44 and Oct4 were also observed to be upregulated in Clonospheric HCT116 as compared to Parental HCT116 (Fig. 3a and 3b) Decreasing CD24 expression in clonospheric HCT116 was observed which correlated well with increased stem cell expression (Fig. 3b).

Perivascular cells, including pericytes in the smallest blood vessels (e.g., microvessels) and ARCs around larger ones, express mesenchymal stem cell markers and bear a multi-differentiation fate potential (differentiate into osteoblasts, chondrocytes, adipocytes, smooth muscle cells and myocytes) similar to that documented for MSCs in vitro.

Immunocytochemical staining for Ki67 (cell proliferation marker), Sox2 (neural stem cell marker), Olig2 (progenitor cell marker), and GFAP (glial fibrillary acidic protein, a marker for differentiated astrocytes) captured by confocal microscopy imaging.

It was reasoned that if some of the cells in the periphery could represent stem - like cells, then the use of a stem cell marker might indicate their presence.

The stem cell marker CD133 meets the endosomal compartment — new insights into the cell division of hematopoietic stem cells.

This assay detects cells that are able to give rise to new colonies containing a majority of cells that express stem cell markers.

Phenotypic characterizations reveal high levels of CD44, α2β1 integrin, and β - catenin expression in holoclones, whereas meroclones and paraclones show markedly reduced expression of these stem cell markers.

Staining of regular PC3 cells (not shown) or holoclone - derived PC3 - GFP cultures (Fig. 4B) revealed similarly differential expression patterns of these three stem cell markers.

We have also shown that ΔNp63, a stem cell marker, is not expressed in the human corneal endothelium.

ΔNp63, a stem cell marker, was not detected in the corneal endothelium.

Overall, 15 % of cells co-expressed lineage specific and stem cell markers.

We use two stem cell markers to improve the resolution of the analysis, which sorted the population into GCTM - 2HIGHCD9HIGH, GCTM - 2MIDCD9MID, GCTM - 2LOWCD9LOW, and GCTM - 2 − CD9 − (HIGH, MID, LOW, and NEG).