Sentences with phrase «stem cell phenotype»

Titles: 1) Notch - 1 induces Epithelial - mesenchymal transition consistent with cancer stem cell phenotype in pancreatic cancer cells

Notch - 1 induces Epithelial - mesenchymal transition consistent with cancer stem cell phenotype in pancreatic cancer cells

1) Notch - 1 induces epithelial — mesenchymal transition consistent with cancer stem cell phenotype in pancreatic cancer cells:

High CD44 coupled with low CD24 expression is indicative of stem cell phenotype in clonospheres.

Our work suggests that targeting a specific stem cell phenotype, for example with immunotherapies, could be beneficial in patients with oligodendroglioma.»

Not exact matches

«We're excited about the application of CombiGEM to probe complex multifactorial phenotypes, such as stem cell differentiation, cancer biology, and synthetic circuits,» Lu says.

Most experiments with human stem cells have only shown that the cells can acquire the phenotype of various differentiated cell types, but have not shown that the cells are functional at the transplantation site.

The mouse embryonic stem cell knockout resource provides a basis for the characterization of relationships between genes and phenotypes.

The IMPC envisages a ten year programme to undertake a broad - based, systematic genome - wide phenotyping project of knockout mice generated from the embryonic stem cell mutant resources developed by the International Knock - out Mouse Consortium (IKMC).

Derivation of pluripotent stem cells, either of embryonic origin or following genetic reprogramming, has opened the path for an alternative source for epidermal cell therapy as these cells are both immortal and pluripotent, theoretically capable of providing any requested number of cells of any desired phenotype.

The exploration of the therapeutic potential of stem cells requires the characterization of their biological properties, the deciphering of the mechanisms that underlie their pluripotency and their capacity at differentiation, by the understanding of the signals that direct their fate towards discrete cell phenotypes... Their therapeutic use requests further, in particular in the case of substitutive therapies, the analysis of their capacities of integration in injured adult tissues and of their potential tumorigenicity, as well as the development of original ways of delivery.

Here, Anna presents how studies of neural stem cells and neurons derived from iPS cells of patients show faithful mimicking of known disease phenotypes in our cellular models of disease, like Alzheimer's disease, autism, and Down syndrome.

Adipose - derived mesenchymal stromal / stem cells: An update on their phenotype in vivo and in vitro (July 26, 2014) in World Journal of Stem Cestem cells: An update on their phenotype in vivo and in vitro (July 26, 2014) in World Journal of Stem Ccells: An update on their phenotype in vivo and in vitro (July 26, 2014) in World Journal of Stem CeStem CellsCells.

The CCP also focuses on deciphering the regulatory circuit that controls chromatin organization and maintenance in various stem cell types to determine their functional influence on a cell expression signature and phenotype.

Interaction of human mesenchymal stem cells with cells involved in alloantigen - specific immune response favors the differentiation of CD4 + T - cell subsets expressing a regulatory / suppressive phenotype.

IGF prevents frailty by increasing skeletal muscle mass (sarcopenia), sex drive (infertility), brain thymus (immunosenescence, centenarians maintain a strong immune system), skeletal bone mineralization and marrow stem cell formation (osteoporosis and immune system by bone marrow immune cells working in tandem with thymus and lymphs nodes), I understand that diabetes, an accelerated aging phenotype, is insulin IGF and blood glucose driven.

Critical issues include: (i) heterogeneity in stem cell populations (ii) regulation of cell fate choices; (iii) declining tissue performance with age and exposure to environmental injuries; (iv) the use of iPS and Embryonic Stem (ES) cells, and reprogramming methods for phenotyping disease states and potential use of these stem cells in the clistem cell populations (ii) regulation of cell fate choices; (iii) declining tissue performance with age and exposure to environmental injuries; (iv) the use of iPS and Embryonic Stem (ES) cells, and reprogramming methods for phenotyping disease states and potential use of these stem cells in the cliStem (ES) cells, and reprogramming methods for phenotyping disease states and potential use of these stem cells in the clistem cells in the clinic.

In our study, we found that exposing metastatic breast cancer cell lines to hypoxia and reoxygenation cycles induces a unique subpopulation that is highly metastatic and exhibits stem - like and EMT phenotypes.

Other groups have also reported that exposing cancer cells to environmental factors such as serum deprivation or hypoxia alone can increase the stem - like phenotype or the number of stem - like cancer cells [40, 48, 49].

Furthermore, we found that the stem - like and EMT phenotypes observed in the cycling hypoxia - selected subpopulation are not reversible, because we obtained the same molecular profile from this subpopulation by culturing them as spherical cultures for several months in normal oxygen content and culturing them as adherent cells (Figure S2 in Additional file 2).

We also found that cells derived from this novel subpopulation form colonies readily, are highly tumorigenic in immune - deficient mice, and exhibit both stem - like and EMT phenotypes.

Our results led us to speculate that there might be a metastatic subpopulation within stem - like cancer cells since other studies have shown that CSC phenotype alone may exhibit invasive property in vitro but is inadequate to determine or predict in vivo metastasis.

Stem Cell Differentiation: Electrical Differentiation of Mesenchymal Stem Cells into Schwann - Cell - Like Phenotypes Using Inkjet - Printed Graphene Circuits by Suprem R. Das, Metin Uz, Shaowei Ding, Matthew T. Lentner, John A. Hondred, Allison A. Cargill, Donald S. Sakaguchi, Surya Mallapragada, Jonathan C. Claussen.

This allows for the identification, evaluation, and isolation of stem and progenitor cells based on aldehyde dehydrogenase (ALDH), expression and not their cell surface phenotype.

The resulting phenotype is highly motile and possesses stem - cell - like properties, a high degree of resistance to treatments, and an increased rate of drug efflux.

Thus, all research on lines of human embryonic stem cells as the cells of various phenotypes derived from these lines should aim to lexploration mechanisms or the development and validation of therapeutic applicable to serious diseases.

I have spent many years studying 2q23.1 deletion and duplication syndrome through genotype - phenotype, neural progenitor stem cells, and RNA - seq studies.

Finally, miR - 181a was recently shown to induce neuronal differentiation and a DA phenotype in human long - term, self - renewing, neuroepithelial - like stem cells [49], which supports the potential role for miR - 181a in regulating development of mDA neurons.

Mouse embryonic stem (ES) cells are a popular model system to study biological processes, though uncovering recessive phenotypes requires inactivating both alleles.

Short - term CRISPR expression would be especially desirable, as it would reduce the potential of off - target editing over time, but this approach would require robust stem cell editing to maintain the desired phenotype.

Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein - tagged cells, assessing apoptosis and cell cycle status, the identification / isolation of stem and cancer stem cells (e.g., marker positive, side - population, etc.) and the deposition of single cells.

However, this is inefficient and has a low throughput method so I aim to use our growing knowledge of epigenetics to devise strategies for «reprogramming» human somatic cells towards a stem cell like phenotype.