Researchers at Emory and Georgia Tech have developed a method for Cheap Oakleys purifying cardiac muscle cells from
stem cell cultures using molecular beacons.
Not exact matches
Bioengineers at the University of California, San Diego have proven that when it comes to guiding
stem cells into a specific
cell type, the stiffness of the extracellular matrix
used to
culture them really does matter.
Using human fetal «mini-brains» grown in 3 - D
cultures, scientists determined that a specific protein produced by the Zika virus changes the properties of neural
stem cells in the developing brain of an infected fetus, potentially causing microcephaly in newborns (Ki - Jun Yoon, abstract 103.06, see attached summary).
«If we win, the entire regulatory structure for autologous
cell processing, with or without
culture, will be rewritten such that any physician
using good practices and treating patients responsibly can
use stem cells as part of his or her medical practice,» he says.
The
cells used to build the organs could be
cultured from
stem cells taken from fat or bone - marrow tissue, he says: «We could engineer a blood vessel from your own
cells.»
The students
used a 3 - D printer to design a smaller and more cost - efficient bioreactor — a miniaturized
culture device where neural
stems cells are grown and eventually become small laboratory brains for research.
The team
used human embryonic
stem cells — which can transform into any
cell of the body — and
cultured them in a mixture of chemicals to grow human brain
cells.
When we first posted this story, we
used the headline: «
Stem cell scientists reveal «unethical» work
culture».
Despite a lack of rigorous clinical trials to test safety or efficacy, a ban of the treatment by health authorities, and a lack of peer review by the scientific community, the privately funded Stamina Foundation has been
using cultured mesenchymal
stem cells that have been exposed to putative conditions that favour neuronal differentiation to treat different diseases in severely or terminally ill patients.
Together with team members Dr. Rouzanna Istvánffy and Dr. Baiba Vilne, Oostendorp
used mixed
cultures of tissue and
stem cells to investigate how the two
cell types interact.
In their work, the researchers also show how TGF - beta can be
used in
stem cell cultures to mass - produce nerve
cells which in turn produce the signalling substance serotonin.
To replicate these
cell culture results, Rani
used human
stem cells to grow neurons into what is called a mini brain.
De Luca's team
used a patch of skin a little bigger than a U.S. postage stamp from an unblistered part of the boy's groin to
culture epidermal
cells, which include
stem cells that periodically regenerate the skin.
«Our group pioneered the development of
cell culture technology for harvesting large numbers of
stem cells from human bone marrow and human umbilical cord blood,» Dr. Yeh said, noting that
stem cells from these two sources are abundant and can be guided into different types of
cells using tissue engineering.
«Our results suggest that megakaryocytes might be
used clinically to facilitate adult
stem cell regeneration and to expand
cultured cells for adult
stem cell transplants,» says Meng Zhao, Ph.D., a postdoctoral fellow at Stowers and lead author on the study.
They
used a technique pioneered at Smith's lab that
uses cultures of various inhibitory proteins to keep embryonic
stem cells continually renewing themselves rather than differentiating into other
cells.
Researchers
used cultured stem cells derived from their feline patients in the hope of reducing inflammation and promoting tissue regeneration.
Unlike many other
stem cell treatments, such as heart patches, the procedure Taylor and his colleagues
used did not require any advance
culturing or growth in the lab.
Surgeons report success in first human bone growth procedure
using fat
stem cells — with no
culturing necessary
In addition, the molecular beacons technique http://www.lependart.com could have broad applications across regenerative medicine, because it could be
used with other types of
cells produced from
stem cell cultures, such as brain
cells or insulin - producing islet
cells.
This information could then be
used to prompt embryonic
stem cells to differentiate in the
culture dish into neurons for potential
use in
cell - replacement therapy.
We
use neuronal
cultures, induced pluripotent
stem (iPS)
cells and transgenic and gene - targeted mouse models to study the differential effects of apoE3 and apoE4 on
cell signaling pathways and cytoskelatal structure and function at molecular, cellular and behavioral levels.
Three - dimensional neuroepithelial
culture from human embryonic
stem cells and its
use for quantitative conversion to retinal pigment epithelium.
Gage's team
used human pluripotent
stem cells to develop brain organoids, which were grown in
culture for 40 to 50 days.
Embryonic
stem cells — the subject of so much controversy (witness the new ruling blocking their
use)-- were first
cultured in the lab a little more than a decade ago; in 2006, there was another breakthrough when adult
cells were coaxed into becoming induced pluripotent
stem cells.
We
use 3D
stem cell («organoid»)
cultures from mice and humans to examine occurrence, level and molecular causes of CIN in cancer (Video 2).
The study
used three
cell types: human motor neurons derived from induced pluripotent
stem cells, rat cortical
cultures, and human breast cancer
cells.
A future aspect of our mouse work is directed towards
use of ES
cell differentiation in
culture as a model for epigenetic decisions and
stem cell manipulations.
His team is
using innovative
cell culture models to advance our understanding of brain
stem cells and their potential role in glioblastoma.
Scientists at the University of Cambridge have managed to create a structure resembling a mouse embryo in
culture,
using two types of
stem cells — the body's «master
cells» — and a 3D scaffold on which they can grow.
Dr. Masayo Yumoto, team leader of Regenerative Medicine Research & Planning Division, Rohto Pharmaceutical Co., Ltd., has been holding a leadership role in the joint research lab at the Institute of Medical Sciences, University of Tokyo, to develop next generation
cell culture method
using human mesenchymal
stem cells.
For instance, MEF
cells are usually made of fibroblasts from the mouse embryos at embryonic day 13.5 and only
cells at early passages (p2 to p3) are
used as feeders for derivation and
culture of embryonic
stem (ES) and iPS
cells.
In addition, pluripotent
stem cells tend to be genetically unstable, particularly if less than optimal
culture techniques are
used.
Ultimately, we
use all this information to establish innovative
culture techniques for generating unlimited neural
stem cell sources for the derivation of specific types of clinically relevant neuronal or glial
cells, for their
use in disease modelling and drug discovery platforms, and towards refining their efficacy for future
cell replacement - based therapy.
Our improved IVM
culture conditions may be
used for obtaining mature oocytes for clinical purposes and / or for derivation of embryonic
stem cells following parthenogenesis or nuclear transfer.
When Po - Lin So contacted me about the position in Gladstone's
Stem Cell core facility, I realized it was the perfect opportunity to use my experience with iPSC (induced pluripotent stem cell) culture and differentiation, as well as gain new skills and further my career gro
Stem Cell core facility, I realized it was the perfect opportunity to use my experience with iPSC (induced pluripotent stem cell) culture and differentiation, as well as gain new skills and further my career gro
Cell core facility, I realized it was the perfect opportunity to
use my experience with iPSC (induced pluripotent
stem cell) culture and differentiation, as well as gain new skills and further my career gro
stem cell) culture and differentiation, as well as gain new skills and further my career gro
cell)
culture and differentiation, as well as gain new skills and further my career growth.
Cells from these embryos can be
used to create pluripotent
stem cell «lines» —
cell cultures that can be grown indefinitely in the laboratory.
Cortical development and cortical
stem cells The laboratory
uses neural
stem cell culture, transgenic model systems and retroviral vectors to study signalling pathways that are central in mouse cortical development and in particular in cortical
stem cells.
This poster describes the isolation and maintenance in
culture of pluripotent
stem cells, their differentiation, and the generation and potential
uses of organoids.
Ongoing studies include refinements of these early research efforts, as well as exploring new ways to
culture and
use stem -
cell therapies in horses.