And, in the future, the potential to insert barcodes in genes and the development of in - line ID kits, that recognize specific
strains of cell lines, could make it easier to verify new organisms and their protein products, and track products through supply chains.
Not exact matches
Despite the presumed virulence
of the
strain — experiments with mouse lungs showed it produces 1000 times more bacteria in infected
cells than do standard varieties — Valway says the number
of TB cases that developed were kept in
line with other typical outbreaks, which «shows that doing good contact investigations is important and preventative therapy works.»
Human placental
cell line Jeg - 3 can be readily infected by a circulating
strain of Zika virus (left panel) and completely protected by treatment with nanchangmycin (right panel).
For their study, the team flooded a
line of Escherichia coli (a common bacterium that lives harmlessly in the intestinal tracts
of many animals, although some
strains can cause food poisoning with antibiotics, killing some
of cells but leaving behind a gaggle
of stubborn ones.
During the next five years, KOMP2 will transform the knockout mouse embryonic stem (ES)
cells into adult mice for 2,500
lines of well - characterized knockout mice
strains, and IMPC will create about 2,500 additional knockout mouse
strains.
Parental
cell lines - background
strain microsatellite testing can be performed to assure genetic identity
of the mouse
strain of origin.
• ES
cell line purity verification In addition to mouse
strain purity verification, the facility provides genetic screening
of ES
cell lines used for gene targeting experiments.
The Repository
of mouse ES
cell lines,
strains and vectors generated by the trans - NIH KOMP initiative that aims to generate a public resource comprised
of mouse embryonic stem (ES)
cells containing a null mutation in every gene in the mouse genome.
We have gone on to show that the sensitivity
of the germ
line genome instability differs across inbred
strains of mice, offering a unique opportunity to use systems genetics approaches to discover the underlying pathways governing
cell division and survival across a variety
of cell types.
In addition, Dr. Reinholdt's laboratory developed a novel protocol for derivation
of embryonic stem
cell line from a variety
of strains that were previously deemed recalcitrant after years
of failed attempts by the scientific community.
The majority
of gene - targeting experiments in mice are performed in 129Sv - derived embryonic stem (ES)
cell lines, which are generally considered to be more reliable at colonizing the germ
line than ES
cells derived from other
strains.
Analysis
of viral genomic and human mitochondrial sequences revealed that all previously characterized XMRV
strains are identical and that the archival RNA had been contaminated by an XMRV - infected laboratory
cell line.
EMMA
strains produced from ES
cells generated by the International Knockout Mouse Consortium (IKMC) are displayed on the EMMA
strain list as soon as germ -
line transmission has been achieved and the genotype
of the pups has been confirmed.
In rare cases, a much more virulent
strain of this virus can cause inflammation
of the liver, intestines, pancreas, and
cells that
line the blood vessels.