Every tissue in an organism contains a variety of messenger RNA molecules, single -
stranded nucleotide sequences bearing instructions for the making of specific proteins.
Not exact matches
It starts with a long DNA
strand — called a scaffold — that has a precise
sequence of the four molecular units, or
nucleotides, dubbed A, C, G, and T, with which DNA spells out its genetic code.
The software is designed to run on the MinION, an instrument the size of a credit card that pulls in
strands of DNA through its microscopic pores and reads out
sequences of
nucleotides, or the DNA letters A, T, C, G.
Much of this DNA is no longer capable of moving, but is likely «auditioning» perhaps as a regulator of gene function or in homologous recombination, which is a type of genetic recombination where the basic structural units of DNA,
nucleotide sequences, are exchanged between two DNA molecules to repair breaks in the DNA
strands.
To record the incorporation of
nucleotides, a CCD camera takes an image of the light emitted when fluorescently labeled
nucleotides are added to the
sequencing strand.
Four types of reversible terminator bases (RT - bases) are added and the
sequencing strand of DNA is extended one
nucleotide at a time.
After other enzymes «unzip» the double -
strands of DNA, DNA polymerases attach to each
strand and begin to travel down the
sequence of
nucleotides.