(Hayashi S. Resolution doubling using confocal microscopy via analogy with
structured illumination microscopy.
In super-resolution microscopy, the moiré pattern can be used to obtain images with a resolution higher than the diffraction limit, using a technique known as
structured illumination microscopy.
Furthermore, the microscope will be capable of performing live - cell super-resolution imaging through
structured illumination microscopy (SIM) and Super-Resolution Radial Fluctuations (SRRF); for fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization microscopy (SMLM) techniques.
Several more researchers presented their work during the day, including protein synthesis at atomic resolution, bio-imaging opportunities at synchrotrons, multi-dimensional imaging during plant cell differentiation, how to use electron cryomicroscopy for in situ structural biology, and how
structured illumination microscopy can offer insights into the regulation of mammalian meiosis.
The new methods dramatically improve on the spatial resolution provided by
structured illumination microscopy, one of the best imaging methods for seeing inside living cells.
Shroff and his research fellow Andrew York, Ph.D., found an answer to these problems with their new instant linear
structured illumination microscopy (iSIM), described in a paper published in Nature Methods on October 6th.
Traditional linear
structured illumination microscopy (SIM) can not maintain the high resolution desired by researchers when the sample is moving quickly.
This one is called
structured illumination microscopy, SIM, and it falls under the heading of «super resolution.»
Not exact matches
In 2004 Jan Huisken of the Max Planck Institute of Molecular Cell Biology and Genetics and colleagues published a paper establishing
structured plane
illumination microscopy, or SPIM, and the field has been booming ever since.
But now, using a technique called
structured illumination, the EMBL team has managed to subtract out this interference, making light sheet
microscopy even more powerful.
For biologists who study development light sheet
microscopy, especially using
structured illumination, is a godsend.