Sentences with phrase «studies on chromatin»

He later combined it with studies on chromatin, tissue specific gene expression and mouse models for human diseases including Type II diabetes, polycystic kidney disease as well as cancer.

Not exact matches

At the same time, coauthor Peter Adams, from the University of Glasgow, published a previous study on the breakdown of the nuclear lamina in which he observed a peculiar protrusion, or blebbing, of the nuclear envelope into the cytoplasm, and these blebs contained DNA, nuclear lamina proteins, and chromatin (the nuclear structures in which genes reside).

The initial work was mainly focused on the study of the regulation of muscle chromatin and gene expression by motor innervation.

Cancer studies have increasingly focused on chromatin — the intertwined proteins and DNA that are packaged into chromosomes — because of its ability to regulate genes important for either activating or inhibiting tumorigenesis.

To study it, ENCODE utilized two approaches based on chromosome confirmation capture (3C), in which physically interacting regions are cross-linked by formaldehyde, and isolated from non-interacting chromatin with restriction enzymes.

This is in accordance with previous reports that decitabine and 5 - azacytidine produce a marked synergistic effect in combination with suberoylanilide hydroxamic acid and romidepsin in T - lymphoma cell lines by modulating cell cycle arrest and apoptosis.26, 27 As a mechanism of action, KMT2D mutations of B - lymphoma cells promote malignant outgrowth by perturbing methylation of H3K4 that affect the JAK - STAT, Toll - like receptor, or B - cell receptor pathway.28, 29 Here our study indicated that dual treatment with chidamide and decitabine enhanced the interaction of KMT2D with the transcription factor PU.1, thereby inactivating the H3K4me - associated signaling pathway MAPK, which is constitutively activated in T - cell lymphoma.13, 30,31 The transcription factor PU.1 is involved in the development of all hematopoietic lineages32 and regulates lymphoid cell growth and transformation.33 Aberrant PU.1 expression promotes acute myeloid leukemia and is related to the pathogenesis of multiple myeloma via the MAPK pathway.34, 35 On the other hand, PU.1 is also shown to interact with chromatin remodeler and DNA methyltransferease to control hematopoiesis and suppress leukemia.36 Our data thus suggested that the combined action of chidamide and decitabine may interfere with the differentiation and / or viability of PTCL - NOS through a PU.1 - dependent gene expression program.

Such an allelic chromatin organization might be present at other DMRs as well, and a recent study by Schweizer et al. (2) shows that the imprinting - control region in the 5 % portion of the SNRPN gene has strong hypersensitive sites on the unmethylated maternal chromosome only.

Furthermore, genome - wide studies, combining chromatin immunoprecipitation (ChIP) and array hybridization (ChIP - on - chip), have revealed that both active and silenced genes are directly bound in ES cells by one or more of the core pluripotency factors Oct4, Sox2 and Nanog [17], [19], [63].

«In this study, researchers evaluated the effect of dietary fats on one particular chromatin gene known to lengthen roundworm lifespan.