Sentences with phrase «subcellular localization of»

Distribution and subcellular localization of glucocorticoid receptor immunoreactive neurons in the developing and adult male zebra finch brain.

The expression or subcellular localization of DDX3 is altered in tumor cells.

Our results clearly indicate that the expression of DDX3 is decreased in HCC and the subcellular localization of DDX3 is altered in cutaneous SCC (see Figs. 5 and 6).

A and B, phosphorylation and subcellular localization of mTOR signaling proteins in ALCL cell lines.

Deputy Editor Steven Carr of The Broad Institute will talk about new approaches for subcellular localization of proteins and their interaction partners with high spatial resolution.

The researchers also examined the subcellular localization of ApoE proteins in human neuroblastoma cells transfected with either ApoE3 or ApoE4, detecting the molecules in both the cytoplasm and the nucleus.

The subcellular localization of the Niemann - Pick Type C proteins depends on the adaptor complex AP - 3.

Differential subcellular localization of the regulatory T - cell protein LAG - 3 and the coreceptor CD4.

Thus, a change in the subcellular localization of CED - 4 may drive programmed cell death.

Not exact matches

A high - resolution map of the human cell has been generated — part of the Human Protein Atlas database — that provides the in situ localization of 12,036 human proteins at a single - cell level, covering 30 subcellular structures, and enabling 14 major organelle proteomes to be defined.

Her research is at the interface between bioimaging and proteomics, and aims to define the spatiotemporal organization of the human proteome at a subcellular level in an effort to understand how variations and deviations in localization contribute to cellular function as well as disease.

We demonstrated the technique on 20 different biological processes spanning four orders of magnitude in space and time, including the binding kinetics of single Sox2 transcription factor molecules, 3D superresolution photoactivated localization microscopy of nuclear lamins, dynamic organelle rearrangements and 3D tracking of microtubule plus ends during mitosis, neutrophil motility in a collagen mesh, and subcellular protein localization and dynamics during embryogenesis in Caenorhabditis elegans and Drosophila melanogaster.

Open Reading Frame E3 - 10.9 K of Subspecies B1 Human Adenoviruses Encodes a Family of Late Orthologous Proteins That Vary in Their Predicted Structural Features and Subcellular Localization.

Phosphorylation of tumor necrosis factor receptor CD120a (p55) by p42 mapk / erk2 induces changes in its subcellular localization.

Sina Koch (Ehninger, TUD)-- «Aberrant subcellular localization as a potential mechanism contributing to the abnormal signaling of the mutant Flt3 - ITd receptor tyrosine kinase in acute myeloid leukemia» (2007)

Protein subcellular localization profiling of breast cancer cells by dissociable antibody microarray staining.

Because DDX3 exhibits tumor suppressor functions, such as a growth - suppressive property and transcriptional activation of the p21waf1 / cip1 promoter, and is inactivated through down - regulation of gene expression or alteration of subcellular localization in tumor cells, all these features together suggest that DDX3 might be a candidate tumor suppressor.

Lysates from these two cell populations were fractionated on the basis of the subcellular localization using the ProteoExtract Kit, and 40 μg of proteins was loaded for Western blotting.

Additionally, an alteration of subcellular localization from nuclei to cytoplasm was also observed in > 70 % of cutaneous squamous cell carcinoma samples.

Additionally, alterations of subcellular localization through aberrant splicing or genetic mutations also provide ways to inactivate tumor suppressor genes (50).

In the second step, all antibodies targeting the same protein are taken in consideration for final annotation of the subcellular localization.

Basic annotation parameters include an evaluation of i) staining intensity (negative, weak, moderate or strong), ii) fraction of stained cells (rare, < 25 %, 25 - 75 % or > 75 %) and iii) subcellular localization (nuclear and / or cytoplasmic / membranous).

In the PROSPECTS project new or improved tools and approaches have been developed to enable absolute protein quantification, study of protein - protein interactions and protein complexes and the quantitative study of protein abundance and turnover rates in different subcellular localizations at a proteome - wide level.

Questions remain concerning why neurons that apparently express similar innexins (the different motor neuron classes) rarely form gap junctions with one another, if gap junctions are subject to constant formation and reformation in the nervous system, and why there appears to be a consistency in subcellular localization (soma versus process) of certain gap junctions.

Our results are the most accurate subcellular localization classifications to date, and demonstrate the usefulness of deep learning for high throughput microscopy.

Several highly conserved residues in SERT have been shown to be critical for its subcellular localization, and mutation of these sites may contribute to both obsessive - compulsive disorder (OCD) and autism.

The cellular localization of the polymers was confirmed by subcellular fractionation of the cells, followed by digestion with endoglycosidase H (Figure 2C and Supplemental Figure 3C), an enzyme that removes N - linked glycans still in the high mannose ER form but does not affect oligosaccharide chains after the addition of sialic acid in the Golgi apparatus.

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.