Sentences with word «subcloning»

The PCR product was cloned and the isolated Kpn I / Nhe I fragment then subcloned into a pBS sk - plasmid bearing a GFP (afmx) sequence with an Nhe I site at the translational start of GFP.

Psin - miR7 - 2 plasmid was constructed by subcloning PCR product from pCDH - CMV - MCS - EF1 - copGFP - miR7 - 2.

The cDNAs were then subcloned into the pCEP4β episomal plasmid (Invitrogen).

pSIN - KLF4 - puro lentiviral plasmid was constructed by subcloning from pLM - mCherry - 2A - KLF4 purchased from Addgene.

This flash animation shows the process of subcloning.

On further culture or after subcloning this ES cell line at various times (passages), this particular mtDNA haplotype showed essentially random drift, with cells carrying anything from 0 to 100 %.

STAT2 full - length cDNA expression plasmid was obtained by subcloning the insert of pBlueScript - STAT2 (from Dr. J.E. Darnell, Jr.) to the vector pcDNA3.1.

For subcloning, cells were dispensed onto three 96 well plates in concentrations of 300, 100 and 30 cells per plate in a volume of 200 µl CM per well.

Subcloning is part of the process of preparing DNA for sequencing during the Human Genome Project.

Designing viral vectors for gene overexpression can be relatively straightforward in that the gene coding sequence can simply be subcloned into a viral vector (though codon optimization of the sequence could increase protein expression within different species).

Selected Amrose variant fluorescent proteins were subcloned into the pCS2 + expression vector [32](EcoRI - XbaI) using the gel purified amplicons generated above.

A GFP sequence amplified from pPD114.24 (provided by A Fire) with flanking Sal I sites and including a translational stop site was subcloned into the Xho I site; the resultant construct is predicted to delete 98 amino acids from the carboxyl terminus of INX - 19.

This product was subcloned into the GFP vector as a Spe I fragment.

Protein coding sequences were flanked with unique NotI and AscI restriction sites and subcloned into a derivative of the pTT3 expression plasmid between a 5» mouse variable κ light chain signal peptide [32], and a 3» tag consisting of the rat Cd4 domains 3 and 4 followed by an enzymatic biotinylation sequence and a hexahistidine tag [33].