Sentences with phrase «subconfluent culture plates»

This is a culture plate of Lactococcus, milk fermentation tubes and mini-cheeses made in Dr Mark Turner's lab.

After collecting biopsies from banked human donor eyes, the team expanded the numbers of cells in a culture plate using human serum to nourish them.

The research highlights the importance of studying the effects of drugs not only on the tumor cells in a culture plate, but also on the interaction of the tumor cells with their own microenvironment.

Two petri dish culture plates show the interaction of bacteria and antibiotics (on disks).

If the cells grew on culture plates, the team inferred that the human gene could fill in for its yeast equivalent.

The researchers removed the organ of Corti, which is housed within a seashell - shaped cavity called the cochlea and contains sensory hair cells, from newborn mice and kept the cells alive in culture plates.

This protocol video demonstrates how to surface - coat cell culture plates and transwell inserts with Cultrex ® Basement Membrane Extract, also called Cultrex ® BME.

Like a conventional tissue culture plate, DA - HCS plates can be easily coated with DNA, poly - lysine, Collagen, fibronectin, silane - compatible substrates, or any other cell culture coatings.

Comparison of Human alveolar osteoblasts cultured on polymer - ceramic composite scaffolds and tissue culture plates.

Assays for virus - specific neutralizing antibodies were performed on serial 2-fold diluted samples of each serum using 2 % FBS - DMEM as the diluent in 96 - well tissue culture plates (Falcon 3072); the final volume of the serially diluted samples in each well was 60 µl after addition of 120 TCID50 of SARS - CoV in 60 µl into each well.

For cell proliferation assay, 5 × 104 cells were seeded in triplicate wells per cell line per time point in 12 - well tissue culture plates.

The incubation gases are piped directly onto the culture plate through a perfusion lid.

For intracardiac injections, cells were harvested from subconfluent culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28) in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

F1 virgin females were collected, transferred to individual cells of plastic 24 - well culture plates (various manufacturers) and given a single fly host to lay eggs.

«Before, you could in principle test chemical compounds of interest against hundreds of cell lines, but it would require a lab member going to a freezer, taking out hundreds of vials — one for each cell line — and setting up hundreds of tissue culture plates.

Primary cultures were plated on poly - d - lysine (Sigma) coated tissue culture plates.

The cells were then seeded into 12 - well culture plates (1 ml / well; Falcon, Franklin Lakes, NJ).

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

Briefly, iPS - RPE were cultured on gelatin - coated culture plate inserts.

EPCs obtained after isolation generally adhere to culture plates within 6 to 8 hours of isolation.

HEK293T cells were seeded at 2 × 107 cells in 15 - cm tissue culture plates and cells were treated with 20 mM LiCl for 7 hr.

A positive culture result obtained from a tracheobronchial wash does not necessarily imply the presence of a clinically significant airway infection and should not lead to antibiotic therapy, unless there was a pure bacterial culture on a primary culture plate.

Finally, a series of mesmerizing video installations Order, More and Culture Plate # 7 (ALL 2003) featuring emblematic, anonymous figures in patterns, culminates in the monumental Time Left (2002), a grand opera of isolation and connection, in which row upon row of human figures forming a vast wall text in motion, endlessly march across the entire perimeter of a room to an unknowable destination.

After starting to attempt to lift itself on day 6, SB - 1 started to slowly crawl on the agar surface of the culture well on day 9, and started to eat the algal food provided the culture plate on day 13.

Not exact matches

«As we sat next to each other filling our plates with one another's cuisine — our plates were so beautiful — you could taste the culture through the basic food groups,» Tammy Hotsenpiller said.»

Just as the clash of these geological plates causes earthquakes, so we can expect massive cultural earthquakes as the great cultures are forced into closer contact.

Never more evident is the usefulness of our particular congregation than when a community quakes from the faults of ancient ethnic tectonic plates pushing just below the surface of our culture.

In Roof's words, the tectonic plates underlying American culture are shifting.

It is nice to see a different side to the foodie culture of America, we are constantly shown images of massive greasy plates of fast food.

it's a blend of culture and religion — Lenten fasting (and avoiding the whole meat deal on Fridays) lends itself to plates of meatless, delicious pierogi.

This month to celebrate my heritage, my culture, my family I would like to share the plates of my life.

Martinez says the new license plates will send a strong message about the significant role that chile plays in the state's culture.

Tatura is home to the Tatura Racecourse Reserve, where the Tatura & Shepparton Racing Club Inc hosts a minimum of three full TAB race meetings each season, including the iconic Italian Plate Festival in December - a celebration of the local Italian community and culture - and the Tatura Easter Cup.

Written by our friend Rachael of La Fuji Mama The Hawaiian plate lunch is an institution in Hawaiian food culture — an inexpensive plate of comfort food that can be purchased at restaurants and roadside stands all over the state.

He draws from diverse cultures to create richly textured shareable plates, which encourage a warm, social dining experience.

The small plate «Cabana Bites» will be a celebration of island food cultures incorporating Caribbean, Asian, South American, and Puerto Rican influence.

«State Plate, an INSP original series, takes viewers on a tour of the country with host Taylor Hicks, the «American Idol» winner, platinum selling artist and restaurateur, for a look at the culinary culture of some of America's «most intriguing cuisine.»

We'd love to share the culture and flavor of Fogo's Brazilian - inspired small plates menu and gaucho chef secrets that will interest your viewers / readers.

As a family, we travel to potlucks, BBQs, and parties with out own set of plates and utensils (including cloth napkins); however, I do recognize paper plates are part of American culture.

Our culture gives us the message that if we're not busy, if we're not rushing, if we don't have too much on our plates, then there's something wrong with us.

This is the plate in our culture,» he said.

During a three - hour grilling at the culture select committee, disrupted by a protester throwing a plate of shaving foam, the once all - powerful News Corp chairman and chief executive told MPs: «I am not responsible.»

US Attorney Bharara also challenged other New York politicians to «step up to the plate» to fight what he says is the pervasive culture of corruption rife with «dirty politicians».

Samples collected from surfaces and from the air are cultured on plates containing a growth medium, one specific for bacteria and another for fungi.

But if the quantity of the protein was reduced slightly, the culture yielded a mixture of floor plate cells and dopamine neurons (Neuron, vol 15, p 35).

Companies making high - content cell imaging equipment such as confocal microscopes and plate readers are now adding software tools to process images of 3D cell cultures.

I found that culturing cardiac valve cells on synthetic hydrogels preserves their normal properties better than the traditional plastic plates do, and I discovered a signaling pathway connecting the stiffness of the supporting scaffold to the cells» cytoskeletal structure.

«In the last few years, it has become possible to order cultures of human cells for testing, but they're grown on a plate, a two - dimensional environment,» says Radisic.

Examining agar plates cultured from anthrax in the letters, investigators spotted mutant colonies and then sequenced them.