Monitoring of
subpopulations by trapping confirms a continuing decline, except that abundance remains relatively high on Bruny Island.
Finally, we characterised the resultant memory B cell
subpopulations by IgH sequencing and flow cytometry.
Precision drives could exclusively affect particular species or
subpopulations by targeting sequences unique to those groups.
Therefore, to overcome chemoresistance mediated by EMT, drug screening efforts have focused on eliminating the mesenchymal
subpopulation by searching for drugs with preferential cytotoxic effects on mesenchymal, rather than epithelial HMLE subpopulations [7].
Furthermore, we found that the stem - like and EMT phenotypes observed in the cycling hypoxia - selected subpopulation are not reversible, because we obtained the same molecular profile from
this subpopulation by culturing them as spherical cultures for several months in normal oxygen content and culturing them as adherent cells (Figure S2 in Additional file 2).
Not exact matches
Their population assessment revealed that the central Oregon population of coastal martens is likely fewer than 87 adults divided into two
subpopulations separated
by the Umpqua River.
«
By surveying thousands of individual cells, we were able to define the transcriptional landscape of lung - resident ILCs, observing changes in discrete
subpopulations,» said Kowalczyk.
As
subpopulations moved into isolated areas, how did they remain isolated over a long enough time for new species - defining traits to arise in nuclear genes and become enriched
by natural selection to permit speciation?
The precarious state of those mammals is underscored in a multinational study led
by a University of Washington scientist, published this week in Conservation Biology, assessing the status of all circumpolar species and
subpopulations of Arctic marine mammals, including seals, whales and polar bears.
Itch, but not pain sensation, is abolished
by selective ablation of a small
subpopulation of spinal neurons.
Future studies are needed to examine sodium trends
by race / ethnicity and income to identify vulnerable
subpopulations that further interventions should target,» the article concludes.
Published
by Noha Sharafeldin, M.D., M.Sc., Ph.D., instructor in UAB's Institute for Cancer Outcomes and Survivorship and Division of Hematology and Oncology, this study helps add a missing piece to a long - unsolved puzzle about post-transplant effects on recipients, specifically that vulnerable
subpopulations of similar transplants can benefit from targeted interventions in the years after they receive their lifesaving treatment.
The report
by American Cancer Society investigators calls high rates of smoking among specific
subpopulations one of the most pressing challenges facing the tobacco control community.
The other
subpopulation preferentially imports lactate, using it as the main energy source
by harnessing part of the citric acid cycle.
Together, these results suggested that EMT contributes to the establishment of a small cell
subpopulation in malignant tumors; this population is then capable of driving the regeneration of the tumor, even when the bulk tumor cell mass is destroyed
by therapeutic regimens.
Encouragingly, whole BM reacted similarly to purified hMSCs, as evidenced
by the upregulation of Runx2 and the sustained osteogenic enhancement of the plastic - adherent hMSC - containing BM
subpopulation.
This is at least suggested
by the observation that the mesenchymal
subpopulation in the HMLE model remains viable upon most chemotherapeutic treatment regimens, under conditions that allowed the effective elimination of the epithelial population [7].
These findings are in agreement with other studies on cell morphology [3, 4] and senescence [5], and the authors hope to advance these studies
by separately analyzing
subpopulations derived from the same bone marrow sample, and also surveying the grade of cell maturity (surface receptor analysis) and cytoskeleton composition of the cells during cell tracking.
Gate control of mechanical itch
by a
subpopulation of spinal cord interneurons.
Numerous studies have demonstrated activity of these agents against a wide variety of cancers, especially cancer stem cell - like
subpopulations,
by a host of different mechanisms which comprise inhibition of signaling pathways, of mitochondrial respiration, as well as of cellular stress responses and others.
Here, we show that a non-adherent, stem - like, and metastatic CSC - enriched
subpopulation could be isolated
by exposing human metastatic breast cancer cell lines to cycles of chronic hypoxia followed
by reoxygenation.
Quantitative analysis of Snail and vimentin protein expression
by Western blotting revealed a 3 - to 4-fold increase in nuclear Snail expression and an 8 - to 9-fold increase in vimentin expression in the MDA - MB 231 F3 and BCM2 F3 cells compared with the parental and hypoxia - exposed A3
subpopulations (Figure 6c).
A clustering analysis of mutant allele frequencies suggested that there were four clones (tumor
subpopulations) in the primary tumor defined
by distinct sets of mutations.
(6) Moreover, subcutaneous adipose of prematurely - aged transgenic mice exhibited high levels of staining for the senescence marker senescence - associated - β - galactosidase (SAβ - gal) and expressed high levels of several established markers of senescence, including p21, p19, interleukin - 6, (insulin - like growth factor binding protein - 2 (Igfbp2), and Pai - 1; primary BubR1H / H; INK - ATTAC mouse embryonic fibroblasts forced artificially into senescence
by oncogenic Ras or serial passage exhibited a
subpopulation that was both GFP + and stained positively SAβ - gal.
Sex difference in physical activity, energy expenditure and obesity driven
by a
subpopulation of hypothalamic POMC neurons
The percentage of CSCs in the cycling hypoxia selected
subpopulation was analyzed based on the CD44, CD24, ESA, and E-cadherin expression
by three - color flow cytometry.
When BubR1H / H; INK - ATTAC mouse bone marrow cells were pushed into senescence in vitro
by the PPAR - γ - activating drug rosiglitazone, a
subpopulation of the cells exhibited high levels of INK - ATTAC expression and GFP, coupled with SAβ - gal staining; subsequent to treatment with the INK - ATTAC activating drug, these cells rapidly entered into apoptosis, and within 48 h were either destroyed or in the cell death process.
Cells were collected from either the media (floating
subpopulation) or the adherent
subpopulation after each hypoxic cycle and dissociated into single - cell suspension
by trypsin / EDTA (trypsin / ethylenediaminetetraacetic acid).
To compare the non-adherent population described
by Phillips and colleagues [47] and our F3 non-adherent
subpopulation, we studied the expression level of Snail, an EMT transcription factor, in these two cell populations.
Furthermore, the cycling hypoxia - selected F3
subpopulations had increased mRNA levels of three mesenchymal transcription factors - Snail (7 - to 10-fold), Slug (2 - to 3-fold), and Twist (2 - to 4-fold)- as measured
by qRT - PCR (Figure 6d).
We show that
subpopulations of human astrocytes, generated
by activation of different signaling pathways in the same population of human glial precursor cells, have markedly different effects when transplanted into the injured spinal cord.
First, biological aging causes the limited pool of immunological killer T - cells to be slowly taken over
by defective («anergic»)
subpopulations that are no longer able to carry out their specialized work, and suppress the effectiveness of the rest of the system.
Now, researchers from the Broad Institute's Imaging Platform, Swansea University's College of Engineering, and fellow international collaborators have found a new way to detect these cellular
subpopulations,
by applying machine learning to the hidden information in images of unlabeled cells generated from image flow cytometry.
The resemblance of the right - lateralised activation profile in processing non-local dependencies in music with the left - hemispheric system in language moreover suggests that processing hierarchical structures in music and language involves qualitatively similar mechanisms subserved
by domain - specific neural
subpopulations.
Both transient transfection with Lipofectamine and transduction with adenovirus resulted in a
subpopulation of cells that experessed human IgG, as shown
by the increased fluorescence intensity.
Figure S1 shows QRT - PCR data for expression of the TGF - beta superfamily member GDF3, a gene which is strongly downregulated across the various
subpopulations, for four ES cell lines subjected to separation
by flow cytometry.
Anopheles gambiae was genetically partitioned into inland and coastal
subpopulations, separated
by a central region dominated
by A. coluzzii.
We hypothesised that breast CSC numbers would be altered in hypoxia via dedifferentiation of non-CSC or
by expansion of the CSC
subpopulation.
27 Studies cited
by the 2010 DGAC Report demonstrate varied metabolic responses to lowered dietary saturated fat, with certain
subpopulations exhibiting adverse rather than improved health outcomes.3 Two recent comprehensive meta - analyses indicate that saturated fat is not linked to heart disease.28, 29 In fact, in a definitive review of forty - eight clinical trials, with over sixty - five thousand participants, the reduction or modification of dietary fat had no effect on mortality, cardiovascular mortality, heart attacks, stroke, cancer, or diabetes.30 Yet, avoiding saturated fat remains a cornerstone of national dietary guidance.
These subgroup ratings weigh heavily in the overall performance rating for a school or district because the rating given
by the state is based on the lowest performance on any single criterion (TAAS, dropout rate, attendance rate) for any
subpopulation.
Similarity
by racial makeup calculates the five cities with the lowest total differences in Hispanic, Black, White, and Asian
subpopulation percentages using a euclidean distance calculation, or the difference between two points using a straight line.
Furthermore, these student - centered tools had a stronger showing in the data disaggregated
by subpopulation.
These student - centered uses had a stronger showing in the data disaggregated
by subpopulation.
Multiple, appropriate measures of student performance quantify Results, with sufficient detail to help the team look at the impact of changes in student performance over time
by level, subject and
subpopulations.
Test results were disaggregated
by key student subgroups, such as ELLs and low - income students, and accountability targets were applied to these
subpopulations within schools.
Polar bears have experienced those supposedly deadly low - ice summers for almost a decade but the global population did not drop
by 2/3 as predicted and not a single one of the ten
subpopulations predicted to be extirpated under those conditions has been wiped out.
Recent research
by the International Union for Conservation of Nature (IUCN) Polar Bear Specialist Group, 8 populations are declining, 3 are stable, 1 is increasing, and there is insufficient data to assess the other 7
subpopulations.
These declining PBSG estimates also went viral, and websites such as the one run
by psychologist John Cook, who is now part of the well - funded Center for Climate Change Communication, posted an article concluding, «Current analysis of
subpopulations where data is sufficient clearly shows that those
subpopulations are mainly in decline» and thus support the ESA listing of polar bears as threatened.
This natural variation in population size is seldom mentioned
by those who rush to blame all polar bear
subpopulation declines on recent increases in the open water season.
This is the process
by which winners and losers in the competition for resources bifurcate into two
subpopulations.