In addition, where cells derived from embryonic stem cells are great at proliferating — a potentially critical feature if one wants to grow
sufficient numbers of cells for clinical use — ones from the iPS lines were much feebler.
«Before this study, it was not known if it is possible to produce
sufficient numbers of these cells and successfully use them to remuscularize damaged hearts in a large animal whose heart size and physiology is similar to that of the human heart,» said Dr. Charles Murry, UW professor of pathology and bioengineering, who led the research team that conducted the experiment.
Once endothelial cells are removed from a heterogeneous population of cells, it is often difficult to obtain
a sufficient number of cells for experimental analysis.
Not exact matches
Folate deficiency can result in several different complications — the most important
of these are neural tube defects (such as spina bifida, an abnormality
of the spine and spinal cord) in babies and anemia (in which the
number and function
of red blood
cells is affected leading to an inability
of the blood to carry
sufficient oxygen).
In addition to normal ovarian development, the increased
number of gonocytes (fetal and neonatal germ
cells) is
sufficient for male - to - female sex reversal in XY medaka.
Acquiring a
sufficient number of progenitor
cells has been difficult, however, which is why scientists have considered induced pluripotent stem
cells (iPSCs), since they can be expanded at significantly high levels and then differentiated into the progenitors.
But it hasn't been possible to generate
sufficient number of hair - follicle - generating stem
cells — until now.
Our goals are to understand and overcome the limitations
of regeneration in the mammalian retina, so that endogenous retinal
cells are reprogrammed into an adult stem
cell and generate adequate progenitor progeny to provide
sufficient cell numbers and types for neuronal regeneration.
Once a
sufficient number of virus particles have been generated by the packaging
cells, they can then be harvested and used to transduce target
cells of interest.
Since very few studies have demonstrated the formation
of macro-metastasis from low
numbers of sorted CSCs and currently proposed CSC markers might not be
sufficient to identify all stem
cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the
cell - sorting strategy based on putative stem
cell surface markers.