The research involved Professor Frederic Meunier's laboratory at QBI, where
super-resolution microscopy techniques enabled the researchers to understand how the anaesthetic worked on single cells.
But current
super-resolution microscopy techniques do not deliver spectral information, which is useful for scientists to understand the behavior of individual molecules, as well as to enable high - quality multicolor imaging of multiple targets.
Given that both McSA1 and pab27576 gave robust fluorescent signals, we were able to image them by SIM
a super-resolution microscopy technique permitting a resolution of ~ 100 nm in x, y and of ~ 300 nm in z [44].
Not exact matches
Then, they looked at pairs of proteins in the complex using
super-resolution microscopy — a special kind of
microscopy technique that can discern much smaller things than a traditional optical microscope can — to systematically identify when each protein disassembled.
Both PALM and stimulated emission depletion (STED)
microscopy, the other
super-resolution technique recognized with the 2014 Nobel Prize, illuminate samples with so much light that fluorescently labeled proteins fade and the sample is quickly damaged, making prolonged imaging impossible.
A team led by Professor Theo Lasser, the head of the Laboratory of Biomedical Optics (LOB) at EPFL has now made strides to address the issue by developing a
technique that can perform both 3D
super-resolution microscopy and fast 3D phase imaging in a single instrument.
Super-resolution microscopy is a
technique that can «see» beyond the diffraction of light, providing unprecedented views of cells and their interior structures and organelles.
The authors also employed other advanced imaging
techniques — including electron
microscopy and
super-resolution imaging — to discover that the formation, and subsequent loosening, of these contacts is regulated by a lysosomal protein called RAB7.
Another
technique pinpoints the locations of proteins within their subcellular context by coupling
super-resolution imaging with electron
microscopy.
Optical imaging and spectroscopy, advanced optical imaging
techniques in particular
super-resolution fluorescence
microscopy, optical physics, nanotechnology, single - molecule biophysics and macromolecular biochemistry, single - molecule imaging, gene expression, computational modeling, single - molecule (force) spectroscopy, biomolecular engineering, nucleic acid nanotechnology
Her current primary interest is the development of new
super-resolution localisation
microscopy techniques, both through the development of optical systems and the creation of novel image analysis algorithms.
Furthermore, the microscope will be capable of performing live - cell
super-resolution imaging through structured illumination
microscopy (SIM) and
Super-Resolution Radial Fluctuations (SRRF); for fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization
microscopy (SMLM)
techniques.
«This
technique also later aided the development of several
super-resolution microscopy methods, which earned other researchers a Nobel Prize in chemistry in 2014,» says Yildiz.
In
super-resolution microscopy, the moiré pattern can be used to obtain images with a resolution higher than the diffraction limit, using a
technique known as structured illumination
microscopy.
Compared to previous years, the course has an updated program of lectures involving the latest
microscopy imaging
techniques, such as
super-resolution light
microscopy (SIM / PALM, STED, STORM) or Atomic Force
Microscopy (AFM).