Sentences with phrase «supernatant at»

To perform the virus neutralization assay, 55 µL of viral supernatant at a concentration of 100 TCID50 and 55 µL of serum (starting at a 1 ∶ 8 dilution) were mixed and incubated for 1 hour at 37 °C.

For each pool, 55 µL of rabbit sera and 55 µL of viral supernatant at a concentration of 100 TCID50 were mixed, incubated for 1 hour at 37 °C, and inoculated onto A549 cells in wells of a 96 - well plate as described above.

Not exact matches

By securing a pellet (with neodymium magnets) on the sides of the tube walls rather than at the bottom, it allows complete removal of the supernatant without touching the pellet.

Maternal plasma previously acidified 1 ∶ 1 with ice - cold 10 % metaphosphoric acid (MPA) was centrifuged and the supernatant stored at − 70 °C.

Homogenates were cleared by centrifugation at 100.000 g for 1 h at 4 °C, and supernatants were collected for further analysis (TBS - soluble fraction).

Centrifugation at 100.000 g was repeated and supernatants were collected (FA - soluble fraction).

To remove hepatocytes, homogenates were centrifuged at 300 rpm for 3 min, and then supernatants were centrifuged at 1500 rpm for 10 min.

For the total input control, 20 % of the total supernatant was saved and frozen at − 80 °C.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

Forty - eight hours after transfection, the media were centrifuged at 800 g for 3 min and the supernatant was transferred into new tubes.

After centrifugation at 3,000 g for 20 min, the supernatants were filtered through a 0.2 - µm syringe - driven filter.

After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).

Supernatants were stored at − 80 °C until the moment of analysis.

Cells were incubated with GCTM2 hybridoma supernatant (mouse IgM) for 30 min at room temperature following by three washes with 100 mM Tris - HCl, pH 8.5.

On the following day (considered day 0) the concentrated retroviral supernatants were thawed and mixed at a 20x OCT4, 10x SOX2, 10x KLF4, 10x cMYC ratio, supplemented with fresh MEF media up to 2 ml volume (per well) and 8 ng / ml polyprene and then exposed to the HUF1 cells at 37 °C and 5 % CO2.

Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.

Afterwards, the supernatant was removed by a short spin of the plate bottom up at no more than 9 × g. To wash the pellet, 50 µl of 70 % ethanol was pipetted into each well and again the plate was spun bottom up at no more than 9 × g for 5 — 10 sec.