Sentences with phrase «supernatant by»

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

Not exact matches

Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone - releasing factor in the femtomole range.

By securing a pellet (with neodymium magnets) on the sides of the tube walls rather than at the bottom, it allows complete removal of the supernatant without touching the pellet.

To confirm the generation of infectious virus, viral supernatants were quantitated by an end - point dilution assay.

After the third wash, the supernatant was discarded down to 100 µl and 650 µl of Tris - HCL buffer was added followed by 250 µl of fresh / frozen reduction buffer (100 mM Tris - Cl pH 7.4, 40 mM DTT).

Angiopoietin - 2 protein levels of cell culture supernatants were measured by ELISA (R&D, #DANG20) according to the manufacturer's instructions.

Homogenates were cleared by centrifugation at 100.000 g for 1 h at 4 °C, and supernatants were collected for further analysis (TBS - soluble fraction).

Five hours later, the supernatants were collected to determine cytokine levels by ProcartaPlex Multiplex Immunoassays.

Cell supernatants were separated by SDS - PAGE on a 4 — 15 % gradient gel (Bio-Rad; Hercules, CA).

Cell supernatants were analyzed for IL - 22 production by ELISA.

Cell lysates were analyzed for RNA and supernatants were analyzed for secreted IL - 22 by ELISA.

Cell supernatants were analyzed for IL - 22 secretion by ELISA.

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).

After 2 days, the viral supernatant was collected by spinning and passing through a Millex - HV 0.45 um filter unit (Millipore).

Cells were incubated with GCTM2 hybridoma supernatant (mouse IgM) for 30 min at room temperature following by three washes with 100 mM Tris - HCl, pH 8.5.

Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.

Afterwards, the supernatant was removed by a short spin of the plate bottom up at no more than 9 × g. To wash the pellet, 50 µl of 70 % ethanol was pipetted into each well and again the plate was spun bottom up at no more than 9 × g for 5 — 10 sec.

Immunoglobulin - containing HB1.F3.H2IgG NSC culture supernatant was subjected to purification by protein A affinity chromatography.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).

These changes were accompanied by severe EC damage, decreased E-cadherin RNA level, elevated IFN - gamma in splenocyte culture supernatant, and production of significant IgM antibody against intestinal microbiota.

Combined culture supernatant and deoxycholate reduced calcium uptake by 39 % (p less than 0.001) suggesting a potentiation of supernatant activity by deoxycholate.

«The supernatant decreased the in vitro uptake of calcium by 15 % (p less than 0.001).