Sentences with phrase «supernatant from»

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

Red histograms on each graph show target cells incubated with supernatant from unmodified HB1.F3 NSCs as a negative control.

Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).

HeLa cells were infected with supernatant from the murine macrophage cell line RAW264.7 (ATCC, Manassas, VI) known to express polytropic and ecotropic MuLV (PMLV and EMLV, respectively).

Supplied as cell free supernatant from H9's or infected H9 cells.

Stimulation of pericytes with supernatants from either Ang2 overexpressing HUVEC or supernatants from control HUVEC resulted in significantly increased pericyte migration following Ang2 stimulation (Fig. 2c, Supplementary Fig. 4c, d).

Modality of access under this proposal: Users can provide human or animal serum, plasma or tissue samples, or cell pellets or supernatants from cell models.

In addition, we showed that, although the supernatants from each of the HCMV - infected cultures contained infectious virus, the viral copy number and the quantity and timing of virus production varied among the various organ - specific MSC.

Not exact matches

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

Mcc was purified from the culture supernatants of E. coli VCS257 cells harboring pJEM15 plasmid, as described earlier (Bieler et al., 2005).

(E and F) Using ELISA, IL - 2 and IFN - γ production were measured from culture supernatants of (D) after 72 h of stimulation.

Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene from HIV - 1 (Bx08) expressing gp120 protein in (VERO) cell culture supernatant.

IFN - γ and IL - 2 production from supernatants of individual wells was determined using an ELISA (BioLegend, San Diego, CA).

Loaded gesicles can then be collected from the supernatant and added to target cells for efficient editing with no footprint.

Gesicles can be collected from the supernatant, yielding a concentrated stock of your Cas9 - sgRNA gesicles.

One example is SPF, or supernatant protein factor, which transfers substrate from an inactive to an active pool or from one enzyme site to another.

RNA was also extracted from supernatant of mosquitoes pools as described above and used for the ZIKV rRT - PCR assay.

Spleen CD4 + T cells isolated from BMT recipients on day 21 were treated with vehicle solution or VPA for 24 h, the supernatants were collected, and ELISA was performed to determine the level of IFN - γ (C) and IL - 17A (D).

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

Virus was purified from crude viral supernatant using LentiX Viral concentrator (Clontech) according to manufacturer's instructions.