Sentences with phrase «supernatant protein»

One example is SPF, or supernatant protein factor, which transfers substrate from an inactive to an active pool or from one enzyme site to another.

Phosphorylation of supernatant protein factor enhances wits ability to stimulate microsomal squalene monooxygenase.

Not exact matches

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene from HIV - 1 (Bx08) expressing gp120 protein in (VERO) cell culture supernatant.

Angiopoietin - 2 protein levels of cell culture supernatants were measured by ELISA (R&D, #DANG20) according to the manufacturer's instructions.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

Immunoglobulin - containing HB1.F3.H2IgG NSC culture supernatant was subjected to purification by protein A affinity chromatography.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).