Sentences with phrase «supernatants of»

Furthermore, an increased amount of IL - 2 was present in the supernatants of anti-CD3 — stimulated DKO and Cbl - b − / − — stimulated CD8 + T cells relative to DGKζ − / − CD8 + T cells, which was increased relative to WT CD8 + T cells (Fig. 3E).

IFN - γ and IL - 2 production from supernatants of individual wells was determined using an ELISA (BioLegend, San Diego, CA).

Cultured pericytes abundantly expressed calpain1 (Fig. 2d) and showed a significant induction of calpain1 activity after stimulation with supernatants of Ang2 overexpressing HUVEC (Fig. 2e).

(E and F) Using ELISA, IL - 2 and IFN - γ production were measured from culture supernatants of (D) after 72 h of stimulation.

Mcc was purified from the culture supernatants of E. coli VCS257 cells harboring pJEM15 plasmid, as described earlier (Bieler et al., 2005).

The supernatant of apoptotic cells causes transcriptional activation of hypoxia - inducible factor — 1α in macrophages via sphingosine -1-phosphate and transforming growth factor - β.

RNA was also extracted from supernatant of mosquitoes pools as described above and used for the ZIKV rRT - PCR assay.

The results revealed that the supernatant of 72 - hour ATRA - treated NB4 cells was sufficient to induce the tyrosine phosphorylation of STAT2 and the endogenous RIG - G level in U3A cells, in comparison with the relative consistent level of total STAT2 (Fig. 3B).

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

Not exact matches

«It's an interesting project because backwash supernatant disinfection is not widely used in Australia,» said Michael Bambridge, Managing Director of CST Wastewater Solutions.

Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone - releasing factor in the femtomole range.

By securing a pellet (with neodymium magnets) on the sides of the tube walls rather than at the bottom, it allows complete removal of the supernatant without touching the pellet.

With the current designs of magnetic stands, these purifications can be problematic, often leaving carryover contaminants when the pellet is disturbed or when the supernatant is not completely removed.

After achieving 80 — 90 % confluency, cell culture media were changed to maintenance media with 2 % FBS and were inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.

To confirm the generation of infectious virus, viral supernatants were quantitated by an end - point dilution assay.

For each pool, 55 µL of rabbit sera and 55 µL of viral supernatant at a concentration of 100 TCID50 were mixed, incubated for 1 hour at 37 °C, and inoculated onto A549 cells in wells of a 96 - well plate as described above.

To perform the virus neutralization assay, 55 µL of viral supernatant at a concentration of 100 TCID50 and 55 µL of serum (starting at a 1 ∶ 8 dilution) were mixed and incubated for 1 hour at 37 °C.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

After the third wash, the supernatant was discarded down to 100 µl and 650 µl of Tris - HCL buffer was added followed by 250 µl of fresh / frozen reduction buffer (100 mM Tris - Cl pH 7.4, 40 mM DTT).

Both technologies allow the simultaneous detection of multiple factors in serum, plasma and tissue culture supernatants.

This supernatant was thawed on ice and 50 µl added to 400 µl HPLC grade water, 50 µl of 50 % MPA and 200 µl of HPLC grade heptane.

Phosphorylation of supernatant protein factor enhances wits ability to stimulate microsomal squalene monooxygenase.

Angiopoietin - 2 protein levels of cell culture supernatants were measured by ELISA (R&D, #DANG20) according to the manufacturer's instructions.

Stimulation of pericytes with supernatants from either Ang2 overexpressing HUVEC or supernatants from control HUVEC resulted in significantly increased pericyte migration following Ang2 stimulation (Fig. 2c, Supplementary Fig. 4c, d).

Gesicles can be collected from the supernatant, yielding a concentrated stock of your Cas9 - sgRNA gesicles.

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

Briefly, the appropriate dilution of tissue supernatant was added to the cuvette containing 1.9 mL of 50 mmol phosphate buffer (pH 7.0).

Briefly, 100 ml of culture supernatants were mixed with AVL - Carrier RNA buffer.

Spleen CD4 + T cells isolated from BMT recipients on day 21 were treated with vehicle solution or VPA for 24 h, the supernatants were collected, and ELISA was performed to determine the level of IFN - γ (C) and IL - 17A (D).

For the total input control, 20 % of the total supernatant was saved and frozen at − 80 °C.

Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1) in the supernatant following the co-culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐ culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.

With current designs of magnetic stands, these purifications can be problematic, often with carry over contaminants when the pellet is disturbed or when the supernatant is not completely removed.

After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

1 ml of antibody - containing hybridoma supernatant is only $ 40, significantly cheaper than commercially available antibodies.

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.

Supernatants were stored at − 80 °C until the moment of analysis.

Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).

Importantly, each supernatant labeled MCF7 cells between one and two logs less than either of the HER2 - overexpressing breast cancer cell lines.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

0.1 mL of supernatant was added to BHK - 21 and African green monkey kidney (Vero) cell cultures (ATCC, Manassas, VA) and monitored as above.

Modality of access under this proposal: Users can provide human or animal serum, plasma or tissue samples, or cell pellets or supernatants from cell models.

Single - cell suspension was obtained by filtering the supernatant through a 40 - μm cell strainer, and cell suspension was then gently loaded onto a layer of Histopaque - 1077 gradient (1 × 106 — 3 × 106 cells / mL HistoPaque in a total of 3 - mL volume) and then centrifuged at 400 × g for 30 min at room temperature.

Afterwards, the supernatant was removed by a short spin of the plate bottom up at no more than 9 × g. To wash the pellet, 50 µl of 70 % ethanol was pipetted into each well and again the plate was spun bottom up at no more than 9 × g for 5 — 10 sec.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).

After the supernatant was filtered through 0.45 - μm membrane, it was added to target cells in the presence of 8 μg / ml polybrene (Millipore, Billerica, MA).

In addition, we showed that, although the supernatants from each of the HCMV - infected cultures contained infectious virus, the viral copy number and the quantity and timing of virus production varied among the various organ - specific MSC.

These changes were accompanied by severe EC damage, decreased E-cadherin RNA level, elevated IFN - gamma in splenocyte culture supernatant, and production of significant IgM antibody against intestinal microbiota.

The supernatant, the medium in which the culture is grown, contains a multitude of beneficial byproducts of the growth process, including vitamins, enzymes, antioxidants, and immune stimulators.