Sentences with phrase «supernatants using»
In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.
https://virologyj.biomedcentral.com/
Virus was purified from crude viral supernatant using LentiX Viral concentrator (Clontech) according to manufacturer's instructions.
Not exact matches
«It's an interesting project because backwash supernatant disinfection is not widely used in Australia,» said Michael Bambridge, Managing Director of CST Wastewater Solutions.
Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone - releasing factor in the femtomole range.
UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.
(E and F) Using ELISA, IL - 2 and IFN - γ production were measured from culture supernatants of (D) after 72 h of stimulation.
IFN - γ and IL - 2 production from supernatants of individual wells was determined using an ELISA (BioLegend, San Diego, CA).
Our lab uses this kit routinely to to quantify p24 levels in culture supernatant.
RNA was also extracted from supernatant of mosquitoes pools as described above and used for the ZIKV rRT - PCR assay.
The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.
Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.
The cell culture supernatant was collected and cytokine secretion was determined using the Mouse IFN - gamma Quantikine ® ELISA Kit (Catalog # MIF00), the Mouse IL - 4 Quantikine ® ELISA Kit (Catalog # M4000B), and the Mouse IL - 17 Quantikine ® ELISA Kit (Catalog # M1700).
Thirty - six hours after transfection, the culture supernatants were collected and used for IFNα titration.
UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.