Sentences with phrase «surface markers»

Surface markers provide insurance against possible separation from the boat.

First, we wanted to determine whether the observed regional gradation of expression of cell surface markers of pluripotency was reflected in patterns of pluripotency gene expression, and second, we wanted to compare these results, obtained from cells subjected to minimal manipulation, to those from cells that were separated by flow cytometry in subsequent experiments.

Along with your basic scuba equipment, you'll learn to use various surface marker buoys and floats with lines and reels.

Lipke, who specializes in the structure and function of cell surface markers in yeasts, says Rezende was as independent as a typical late - term Ph.D. student.

In the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expression.

For instance, LKB1 also helped to repress cell surface markers on immune cells called dendritic cells that fueled the allergic reaction.

Most of these CSCs have been identified using surface markers that identify their corresponding normal tissue stem and progenitor cells, thus reinforcing the resemblance of tumor development to normal organogenesis.

Once they were able to isolate skeletal muscle cells using the newly identified surface markers, the research team matured those cells in the lab to create dystrophin - producing muscle fibers.

Positive clones were then differentiated using defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

Using the ERBB3 and NGFR surface markers, the skeletal muscle cells were isolated and then injected into mice at the same time a TGF Beta inhibitor was administered.

Upon further analysis of these fetal muscle cells two new cell surface markers called ERBB3 and NGFR were discovered; this enabled the reserchers to precisely isolate muscle cells from human tissue and separate them from various cell types created using human pluripotent stem cells.

[B] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Most cells that are positive for stem cell surface markers express Oct - 4, but only those towards the top of the hierarchy express the nodal receptor TDGF - 1 and the growth factor GDF3.

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

We extensively analyzed various surface markers (e.g., CD2, CD5, HSA, CD25, class I MHC, etc.) of each stage of thymocyte development, but we did not observe significant differences between Gasp + / + and Gasp − / − thymocytes.

Pack a deployable surface marker if a drift dive is on the schedule.

Cells were washed with 1 % BSA and stained with surface markers for ILC3.

They found that the cancer cells were missing a large region from a stretch of the sixth human chromosome known as 6p, which produces surface markers that immune cells latch on to.

T - lymphocyte cells are distinguished by surface markers.

Significantly, cells with reduced mtDNA became self - renewing and expressed specific cell surface markers characteristic of breast cancer stem cells.

The mouse monoclonal antibody they have developed, MAG - 1, targets the ProAVP surface marker.

«But the desired cells often lack a readily accessible surface marker, or that marker is not specific enough, as is the case for cardiac muscle cells.

MSC control surface marker expression to exhibit a hypoimmunogenic or tolerogenic phenotype.

The authors used the cell surface marker podoplanin as a handle for isolating the progenitor cells from bone marrow.

[B and E] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for leukocyte surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Based on cell surface markers known as CD4 and CD8, T cells generally fall into two broad categories: CD4 — positive helper T cells, which ¬ help activate other immune cells and CD8 - positive cytotoxic T cells, which kill cells that are cancerous or infected with viruses.

Cancer stem cells are remarkably similar to normal stem cells: both self - renew, are multipotent and express common surface markers, for example, prominin 1 (PROM1, also called CD133).

Here we consider two surface markers thought to define these cells in mice, CD8 and Killer cell lectin - like receptor G1 (KLRG1), and a means we developed to remove these cells from the blood of aged C57BL / 6 mice.

ES cells can be described based on a characteristic morphology, the presence of cell surface markers such as SSEA - 1 and Pecam1, or the expression of the key transcription factors such as Oct4, Sox2, Nanog, and a number of ES cell - specific transcripts (ECATs)[4]--[6].

Internalization of POS is observed in a single optical y - axis projection (< 1 µm) of pigmented iPS - RPE cells labelled with the apical cell surface marker ATP1B1 (red).

The samples were stained and examined histologically, and cell surface markers investigated using immunohistochemistry and flow cytometry.

For current dives, stow an inflatable surface marker and deployment reel in your BC.

You will also learn about buoyancy control in a current and special equipment such as delayed surface marker buoys.

Furthermore we hand each diver a 10 ft. long surface marker buoy.

I have been travelling around the work South East Asia - Vietnam, Thailand, Japan and more, collecting Surface Markers along the way.

Enumeration of 33D1 + DCs showed a significant reduction of positive cells in the spleen, confirming that the reduction in CD4 + DCs is due to a loss of this cell type rather than being due to a reduction in surface marker expression (Figure 1F).

You'll also receive this discount on some of the must - haves for the Divemaster course such as a compass, dive computer, surface marker buoy, white slate and tank banger.

g defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

Skeletal muscle cells isolated using the ERBB3 and NGFR surface markers (right) restore human dystrophin (green) after transplantation significantly greater than previous methods (left).

It is of interest that during reprogramming of mouse fibroblasts to induce pluripotency, the cell surface marker SSEA - 1 is upregulated very early on in the process [32].