According to his unpublished findings, when he puts glioblastoma cells from patients into lab dishes with brain organoids, the cells attach to
the surface of the organoids, burrow into them, and within 24 to 48 hours grow into a mass that eventually «looks exactly like what happened in the patient's own brain,» Fine said.
Not exact matches
These «
organoids» can develop as many as six layers
of cerebral cortex — the outer
surface of the brain.
In the
organoids, the scientists found such mechanical instability in two places: the cytoskeleton — the internal skeleton —
of the cells in the center
of the
organoid contracted, and the nuclei
of the cells near the
surface expanded.
These
organoids form all
of the cell types present in human intestine, but they grow as cysts surrounded by thick extracellular matrix gels with their «apical» cell
surface (which is normally exposed to the content
of the gut) facing an enclosed lumen.
These 2D cultures also abolish the need for intraluminal microinjection
of single
organoids and facilitate studies
of host - microbe or virus interactions or the action
of microbial enterotoxins because the luminal
surface is fully exposed to the mucosal medium (22,23).
In the Intestine Chip, the epithelium forms villi - like projections lined by polarized epithelial cells that undergo multi-lineage differentiation similar to that
of intestinal
organoids, however, these cells expose their apical
surfaces to an open lumen and interface with endothelium.