The new round of papers consists of an overview and five papers describing the first assembly of
synthetic yeast chromosomes synII, synV, synVI, synX, and synXII.
A global research team has built five new
synthetic yeast chromosomes, meaning that 30 percent of a key organism's genetic material has now been swapped out for engineered replacements.
The team that built the first
synthetic yeast chromosome has added five more chromosomes to their repertoire, totalling roughly a third of the organism's genome.
In March 2014, Sc2.0 successfully assembled the first
synthetic yeast chromosome (synthetic chromosome 3, or synIII) comprising 272,871 base pairs, the chemical units that make up the DNA code.
She started her postdoc at Johns Hopkins and followed Jef Boeke, the geneticist leading
the synthetic yeast chromosome project, when he moved his lab to NYU.
Not exact matches
A
synthetic version of
yeast chromosome III with every gene tagged can substitute for the original.
Yeast grow on an agar plate in the form of the microbe's
chromosomes, with colors representing whether a
chromosome exists in a
synthetic form (yellow) or just wild - type (orange).
The
synthetic chromosome is based on
chromosome III from the
yeast Saccharomyces cerevisiae, but it is not an exact replica.
As a result of this careful debugging,
yeast cells with the
synthetic chromosomes grow just as quickly in the lab as normal, wild
yeast, despite the wholesale alterations (Science, DOI: 10.1126 / science.aaf4557).
The current work is just 3 percent of the way toward creating an entirely
synthetic yeast genome (there are 16
chromosomes in total) and will take many more years to finish.
With the edits made, the team starts to assemble edited,
synthetic DNA sequences into ever larger chunks, which are finally introduced into
yeast cells, where cellular machinery finishes building the
chromosome.