Sentences with phrase «system cell clones»

«Immune system cell clones created before birth may last for decades: Identical twins may exchange T cells through cord blood before birth.»

Not exact matches

The stem cells produced through this therapeutic cloning would, like other embryonic stem cells, be capable of developing into many cell types and serve as a repair system for whatever part of the body required replenishment at the time.

«In this study, we showed that cancer stem cells co-opt a RNA editing system to clone themselves.

The advent of molecular cloning, DNA sequencing and the many tools of molecular genetics and cell biology has given us sufficient knowledge of the basis for disease and the genes to target, but what has limited the application of gene therapy has been efficient gene delivery systems.

Knockout of CD81 in cells from Cellartis Human iPS Cell Line 18 (ChiPSC18) was performed according to the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System User Manual.

Using the DEF ‑ CS cell culture component of the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System, we achieved an unparalleled survival rate of 6cell culture component of the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System, we achieved an unparalleled survival rate of 6Cell Cloning System, we achieved an unparalleled survival rate of 67 %.

Lloyd Old, Thierry Boon, and colleagues develop the TNF release assay for mouse systems in which release of TNF by T cells could be used to assess specific T cell recognition, facilitating the cloning of human tumor antigens.

This kit utilizes the DEF ‑ CS culture system, recognized for its suitability for genome engineering (Valton et al. 2014) and single - cell cloning (Feng et al. 2014), to promote reliable growth of hiPS cells in a feeder - free and defined environment.

Workflow for targeted knockout of CD81, an endogenous gene in hiPS cells, using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

Table I. Highly efficient survival of edited clones grown in the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

These data demonstrate that the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System enables highly efficient survival of single hiPS cells.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Cell Cloning DEF - CS Culture Media Kit:

Gene cloned from T - cell - tropic HIV - 1 MN strain and the full length recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression System

Single - cell seeding of edited hiPS cells in this system results in high survival of pluripotent, edited clones, yielding a diverse set of edited clonal lines.

The Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System provides an efficient and effective method to generate clonal lines of edited hiPS cells.

Thus, these data show that the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System effectively edits and expands single - cell hiPS cell clones without introducing karyotypic abnormalitCell Cloning System effectively edits and expands single - cell hiPS cell clones without introducing karyotypic abnormalitcell hiPS cell clones without introducing karyotypic abnormalitcell clones without introducing karyotypic abnormalities.

Thus, using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System, we successfully knocked out endogenous CD81 from a starting line and generated 12 new, edited lines that were still pluripotent.

hiPS cells can be cultured, edited, and clonally expanded using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

Using a reverse - genetics system, in which specific viral sequences are manipulated within the context of a full - length infectious clone of the virus, they have been defining the roles of particular viral components in influencing DENV and ZIKV infection in mice and human cells.

To demonstrate Amrose functionality in an established higher model system, Amrose v0 — v4 variants were cloned into the pCS2 + vector system to synthesize mRNA by in vitro transcription, which was used for injection into one - cell stage zebrafish embryos.

We applied our technology to several epigenetic regulators, recovering bi-allelic targeted clones with a high efficiency of 60 % and used Flp recombinase to restore expression in two null cell lines to demonstrate how our system confirms causality through mutant phenotype reversion.

However, it is practically difficult to perform the rescue experiments in the organoid culture system because the lentivirus transfection is required for the gene transduction into the primary stem / progenitor cells and the APC gene (> 8.5 k base pairs) is too large to be cloned into the lentivirus.

The topics covered across the three years are: Biology Humans as organisms - health, diet, exercise, nervous system, disease and control Living processes - respiration, reproduction, transport in cells, Variation, evolution, inheritance & genetics - cloning, mutation and natural selection Ecosystems and habitats - food webs, interdependence and extinction