Sentences with phrase «tag endogenous genes»
These vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains.
http://blog.addgene.org/
Efforts to tag endogenous genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the reasons listed above.
Another way to dodge cellular variation observed in mammalian cells is to tag an endogenous gene.
Not exact matches
Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.
Some examples from their lab include using AAV to introduce epitope tags into the endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008).
Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»
CRISPR has allowed scientists to tag and light up endogenous genes of interest to better understand normal protein function.
In fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR / Cas9 knock - in.
We applied an integrated proteomic and genetic strategy by targeting a tandem affinity purification (TAP) tag and Venus fluorescent protein into the endogenous Arc gene in mice.