Not exact matches
You may have noticed the recent publication «A peptide
tag - specific
nanobody enables high - quality labeling for STORM imaging» of Virant et al (2018) in Nature Communications doi: 10.1038 / s41467 -018-03191-2, where for the first time a peptide -
tag specific
Nanobody was applied in dSTORM imaging: The authors have described and discussed... — Read more
Figure 2: Condensin subunit SMC4
tagged with mEGFP and
nanobody - stained in a prometaphase HeLa cell visualised by an astigmatic 3D STORM microscope.
Label the
Nanobodies: Purified nanobodies are then tagged with a fluorescent dye or HRP via maleimide -
Nanobodies: Purified
nanobodies are then tagged with a fluorescent dye or HRP via maleimide -
nanobodies are then
tagged with a fluorescent dye or HRP via maleimide - labeling.
In the past, we introduced
nanobodies as versatile superresolution labels and pioneered superresolution microscopy in yeast, where strain libraries with
tags and mutations allow system - wide superresolution studies.
Nanobodies will bind to the column due to their N - terminal His14 - bdNEDD8
tag.
Some GFP
nanobodies stain poorly when
tagged with a Spaghetti Monster because they recognize this GFP scaffold itself.