Sentences with phrase «target cell sites»

Not exact matches

In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.

Results from the new study suggest immunostimulant molecules that target neutrophils at nerve injury sites might enhance clean - up and promote nerve cell repair.

«These are optimistic results for one of the first targeted therapies for cancer stem cells,» says Antonio Jimeno, MD, PhD, investigator at the CU Cancer Center, director of the university's Cancer Stem Cell - Directed Clinical Trials Program, and principal investigator of the clinical trial at the CU Cancer Center site.

To address this challenge, our research group is using nanoparticles not only to deliver more chemotherapy drugs to the target site within cancer cells, but also to compromise the function of the efflux pumps and thereby significantly improve safety and efficacy of cancer therapy.»

They cross the blood - brain / blood - tumor barrier, and accumulate within brain tumor sites, where they target oncogenes, regulate cell growth and differentiation, reduce tumor burden and prolong survival in our mouse models.»

In one experiment with human cells, a guide RNA should have led the Cas9 enzyme only to a gene on chromosome 2 (yellow bar), but it also directed the enzyme to many off - target sites (red) on several other chromosomes.

The mushroom - shaped protein specializes in infecting cells, first by binding a trio of sites on its head to three separate sugar molecules on the surface of targeted cells.

disruption of the cell wall barrier by reactions of chlorine with target sites on the cell surface

«We wanted to utilize platelets» intrinsic tendencies to accumulate at wounds and to interact with circulating tumor cells, for targeted delivery of immune checkpoint inhibitors» said Gu, «Interestingly, we found the antibody can be promoted to release from activated platelets in the surgical site, due to generation of small platelet - derived microparticles upon the platelet activation.

Importantly, it's secretion from normal cells can be induced by activating p53 so that Par - 4 enters circulation, thereby potentially targeting tumor cells at distant sites.

These investigations take advantage of white blood cells, monocytes, and macrophages that can target an inflammation site, including inside the brain.

The authors report that their findings represent the first demonstration of a functional link between changes in T cell activation and targeting of specific integration sites by HIV - 1.

Nakano says UAB will be the only site in the Deep South for this unique trial aimed at a molecular target in glioma stem cells, a target that is different from the ones described in the Cancer Research paper.

They found that there was no measurable difference between the concentrations of stem cells in the various samples, despite the fact that the in vivo sample was part of a dynamic environment — which included enzymes and blood flow — making it easier for the stem cells to migrate away from the target site.

The antibody also attracts the third drug, Imprime PGG, which travels to the tumor site to activate an immune response and kill targeted cancer cells.

After they have captured the data at a number of sites they can analyze the data to determine the cell phone or cell phones used by the targeted individual.

They hope to identify the complete set of DNA binding sites and corresponding target genes for the regulons in embryonic stem cells and a subset of the cells they differentiate into.

There are three examples of how law enforcement typically use stingrays for surveillance: First, law enforcement officials may use the cell site simulator with the known cell phone number of a targeted individual in order to determine that individual's location.

A new study shows that a 70 - year - old malaria drug can block immune cells in the liver so nanoparticles can arrive at their intended tumor site, overcoming a significant hurdle of targeted drug delivery, according to a team of researchers led by Houston Methodist.

While additional study is clearly needed, our current studies have clearly identified off - target disruption in two of the top 15 putative off - target sites: an extragenic site on chromosome 12 and in the metalloprotease ADAMTS17, which is not expressed in CD4 + T cells.

While radiotherapy can precisely target just the tumor site, systemic chemotherapy spreads a wide net, sending drugs speeding throughout the entire body in an attempt to kill cancer cells while also killing many healthy cells.

Some of the most important sites targeted by HIV neutralizing antibodies are hidden when the three gp120s, or the trimer, are closed, and the gp120 trimer remains closed until the virus binds to a cell.

Cell growth was monitored every 48 hours post-stimulation for approximately two weeks and the efficiency of CXCR4 disruption was assessed at day five post-transduction by both the Surveyor nuclease assay and by deep - sequencing of the CXCR4 target site.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

This will help us understand how the entire protein works together to block cell death, and may provide new sites to target in drug discovery.»

So instead of taking a shotgun approach to killing a cancer cell, we are using a targeted approach that is not necessarily specific to an anatomical site, but rather the specific mutation identified in the tumor.

Graphical representation of indels created at the CD81 target site in different CRISPR / Cas9 - edited hiPS cell clones.

His thesis was entitled «Quantitative description of microRNA target site occupancy in mouse embryonic stem cells and derived cells of neuronal lineage.»

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.

After analyzing 964 sites cleaved in vitro by different 11 sgRNAs and measuring indel frequencies at hundreds of off - target sites in cells, we propose an off - target scoring system of each target site for minimizing CRISPR - Cas9 off - target effects in the human genome.

Once injected inside a cell CRISPR / Cas9 will bind to the band - tailed pigeon genome at these target sites and cut the DNA.

Furthermore, direct injection in this small and immunologically privileged site can potentially overcome limitations linked to systemic injection which would require a very high dose to target the CNS and can be neutralized by anti AAV antibodies or may trigger the activation of CD8 + T cell response.

Using an enzyme that targets some 200 sites on the X chromosomes in mosquito eggs, the team has managed to shred X chromosomes so thoroughly «that it's too much for the cell to repair,» Burt said.

By injecting the modified viruses into transgenic mice that only express Cre recombinase in astrocytes, the researchers knew that any sequences flagged by the lox site inversion had successfully transferred their genetic cargo to the target cell type — here, astrocytes.

Introduction of DNA double - strand breaks by site - specific endonucleases called meganucleases is being successfully used to target endogenous genes in human cells.

December 13, 2011 Molecular markers can predict spread of cancer, guide treatment Molecular markers found in cancer cells that have spread from a primary tumor to a limited number of distant sites can help physicians predict which patients with metastatic cancer will benefit from aggressive, targeted radiation therapy.

Site - directed mutagenesis by gene targeting in mouse embryo - derived stem cells.

Bringing inflammatory cells to a site of injury enables that injured part to receive nourishment and directs immune activity to its target.

Your pet's own tissue at the targeted site is the scaffold to which the stem cells attach.

Once injected inside a cell CRISPR / Cas9 will bind to the band - tailed pigeon genome at these target sites and cut the DNA.

The agency added that it lacked the funding to «procure, deploy, operate, and maintain» the technical capability to detect cell - site simulators targeting phones on modern 4G / LTE networks.

In other words, when agents get a warrant to use a cell - site simulator against a suspect target, and in doing so inevitably capture private phone information from countless innocent bystanders, the new policy still lets agents use that incidentally captured information against those bystanders if they get court permission.

The DOJ should strengthen its policy and delete any non-target data retrieved by a cell - site simulator as soon as the target is located without reviewing the non-target data acquired.

Then the cell - site simulator targets that IMSI to help police triangulate its location.

EFF has long opposed law enforcement's use of cell - site simulators as incompatible with the protections of the Fourth Amendment because they indiscriminately gather information on countless innocent people who have the misfortune of being in the vicinity of a suspect target.

Cell - site simulators are a form of dragnet surveillance that indiscriminately seize information from everyone in the area, innocent and target alike.

Cell - site simulators, often called IMSI catchers or Stingrays, masquerade as cell phone towers and trick our phones into connecting to them so police can track down suspect targets, but their use also collects the data and location of innocent bystanders and extracts unnecessary sensitive data in the procCell - site simulators, often called IMSI catchers or Stingrays, masquerade as cell phone towers and trick our phones into connecting to them so police can track down suspect targets, but their use also collects the data and location of innocent bystanders and extracts unnecessary sensitive data in the proccell phone towers and trick our phones into connecting to them so police can track down suspect targets, but their use also collects the data and location of innocent bystanders and extracts unnecessary sensitive data in the process.

Much of the evidence collected against him was derived from cell - site simulators targeting his phone.