Sentences with phrase «target gene in a cell»

These «genetic scissors» can be used for targeting any gene in a cell in order to modify it.

In their July 2014 paper, Esvelt and his colleagues described how gene drive would work: CRISPR would cut a target gene in a cell or cells, probably an egg or embryo.

Not exact matches

The technology's possibilities are staggering — in theory, allowing medical scientists to do everything from cure genetic disorders like sickle cell disease to identify gene targets for combating HIV.

I won't reveal yet who my favorites are, but I will say that these young scientist - founders came up with very creative solutions for preventing infections in some common surgeries, tackling resistance in targeted antibody drugs, improving gene vectors for cell therapies, helping the vision - impaired «see» faces and better read their environments, imaging hard - to - see spots in the lungs and other organs, improving genetic risk analysis, and expediting the logistical operations of hospitals.

To discover these targets, the team determined when and where each gene is turned on or off in the cells and tissues of H. contortus to reveal new insights into the worm's lifecycle.

Because the genes that are massively amplified in the neochromosomes are essential for the cell's survival, drugs that turn those off are an obvious target for research.

In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomeIn SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomein embryonic cell genomes.

«Many diseases, especially complex diseases, involve multiple genes, and this system could be used therapeutically to target and activate multiple genes together and rescue these disease phenotypes,» says Albert Cheng, a graduate student in the Jaenisch lab and co-author of the Cell Research paper.

Carlo Croce, a cancer researcher at Ohio State University in Columbus, and his colleagues created a diagram of interacting miRNAs for normal body cells by connecting them according to which genes they target and the function of those genes, in a way similar to analyses of human social networks.

After moving to Berkeley, he arrived at a career crossroads in 1994, when Spyros Artavanis - Tsakonas, then at Yale, discovered and subsequently patented the human relative of the fruit fly gene notch, which plays a role in cell - to - cell interactions and could be an anti-cancer target.

The findings by a team of Massachusetts General Hospital (MGH) investigators, which will be published in the April 24 issue of Cell and are receiving advance online release, support the importance of epigenetics — processes controlling whether or not genes are expressed — in cancer pathology and identify molecular circuits that may be targeted by new therapeutic approaches.

In one experiment with human cells, a guide RNA should have led the Cas9 enzyme only to a gene on chromosome 2 (yellow bar), but it also directed the enzyme to many off - target sites (red) on several other chromosomes.

Gene Yeo, a professor of cellular and molecular medicine at UCSD, led the research and showed he could target RNA in living cells, a first step toward treating diseases like muscular dystrophy and neurodegeneration.

This cell's gene - editing system targets RNA, revealing the molecule's distribution in the cytoplasm.

Gene switches have been identified that work in specific brain areas, potentially enabling targeted treatment of unhealthy cells.

In this way, the team identified a new small protein, growth inhibitor gene product (Gp) 0.6, which specifically targets and inhibits the activity of a protein essential to bacterial cells.

Dwarki and Jaime Escobedo improved the AAV's ability to insert genes into chromosomes by adding a gene promoter region from cytomegalovirus, known to be active in the target for their gene therapy, muscle cells.

In a two - pronged attack, these viruses specifically target tumor cells while delivering a cargo of immune - boosting genes.

CTL119 manufacturing begins with a patient's own T cells, some of which are removed and then reprogrammed in Penn's Clinical Cell and Vaccine Production Facility with a gene transfer technique designed to teach the T cells to target and kill tumor cells.

«Margery's experiments showed that the NS1 protein can alter expression of Hedgehog target genes on its own, without other viral proteins,» said Bier, professor and newly named holder of the Tata Chancellor's Endowed Professorship in Cell and Developmental Biology.

Bach2, an important gene for inducing memory B cells, may become an important target in vaccine strategies.

The former target, say, using gene editing techniques to inactivate HIV receptors and achieve resistance of blood cells to the virus (which Sangamo BioSciences is working on in clincial trials) is different than helping parents who both carry genes for Huntington's Disease to have a child that is free of the disease (a change to the genome that would be passed on to future generations and would likely not be very commonly needed).

These receptors, called receptor tyrosine kinases (RTKs), transmit instructions through the cell wall and down through a cascade of reactions to a target gene in the nucleus.

With high reproducibility, the sgRNAs designed with CrispRGold destroyed the target genes in on average 80 percent of the cells — «an excellent rate,» says Graf.

A molecule in cells that shuts down the expression of genes might be a promising target for new drugs designed to treat the most frequent and lethal form of brain cancer, according to a new study by researchers at The Ohio State University Comprehensive Cancer Center — Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC — James).

Although that marker, called IL21, had not previously been associated with autoimmune diseases, the gene that produces it sits right in the stretch of DNA known to make these mice vulnerable to diabetes, suggesting that IL21 might make a drug target, says Sarvetnick.Furthermore, by giving the animals a shot of dead bacteria — similar to an immunization in humans — when they were newborns, Sarvetnick and her colleagues prevented a surfeit of CD4 + and CD8 + cells.

In an effort to expand the number of cancer gene mutations that can be specifically targeted with personalized therapies, researchers at University of California San Diego School of Medicine and Moores Cancer Center looked for combinations of mutated genes and drugs that together kill cancer cells.

Using in vitro, or test tube, experiments, the researchers applied these chemicals to human cancer cells to measure changes of estrogen receptor - and androgen receptor - target genes and transcriptional activity.

In one experiment this year, a team led by another CRISPR pioneer, Feng Zhang of the Broad Institute in Cambridge, Massachusetts, targeted the 20,000 or so known human genes, turning them on one by one in groups of cells to identify those involved in resistance to a melanoma druIn one experiment this year, a team led by another CRISPR pioneer, Feng Zhang of the Broad Institute in Cambridge, Massachusetts, targeted the 20,000 or so known human genes, turning them on one by one in groups of cells to identify those involved in resistance to a melanoma druin Cambridge, Massachusetts, targeted the 20,000 or so known human genes, turning them on one by one in groups of cells to identify those involved in resistance to a melanoma druin groups of cells to identify those involved in resistance to a melanoma druin resistance to a melanoma drug.

When Cas9 and the short guide RNA targeting a disease gene are delivered into cells, a specific cut is made in the genome, and the cells» DNA repair processes glue the cut back together, often deleting a small portion of the genome.

«Research into basic workings of immune system points to way of improving therapies for cancer: Differences in wiring of «exhausted» and effective T cells indicate possible gene - editing targets.»

«Identifying targets essential to cell survival in tumor suppressor genes has long been an investigational goal with the aim of offering cancer - specific vulnerabilities for targeted therapy,» said Ronald DePinho, M.D., professor of Cancer Biology, MD Anderson president, and senior author for the Nature paper.

His team's approach is based on gene therapy, where a «tame» virus is harnessed to transfer a gene into target cells in the recipient.

One potential treatment for CF is gene therapy, and a major challenge in gene therapy is packaging replacement genes so they can be delivered to the target cells.

But why does WOX5 switch off its target gene CDF4 in stem cells?

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

In 2013, CRISPR passed two important tests: It works in human cells, and it can target several genes at oncIn 2013, CRISPR passed two important tests: It works in human cells, and it can target several genes at oncin human cells, and it can target several genes at once.

«Heart toxin reveals new insights into HIV - 1 integration in T cell genome: Digoxin exposes link between T cell activation and targeted HIV - 1 integration in specific genes.»

In both human airway cells and mouse nasal cells, the researchers observed corrections in the targeted geneIn both human airway cells and mouse nasal cells, the researchers observed corrections in the targeted genein the targeted genes.

If so, it could make cell fate more resilient to random mutations in a plant's genetic code, even when such changes keep some gene - regulating proteins from binding their intended DNA targets.

Intrigued by the ability of certain polymers to mop up DNA and RNA for gene transfer, Sullenger and colleagues tested the idea that these chemical compounds might also be effective targeting such nucleic acids as they arise in cell death.

Gene switches have been identified that work in very specific brain areas, potentially enabling light to target unhealthy cells without disrupting healthy ones.

These include Cenix Bioscience, which has developed a new lab technique to identify the genes that are involved in cell division, predicted to be very useful for finding potential new targets for anticancer drugs.

Research fields are diverse, including basic research on cell proliferation, analysis of tumour cells and tissues to detect gene mutations, and identification of potential therapeutic targets in cancer.

Furthermore, besides offering valuable insight into the function of this novel RNA type, the researchers also believe that the findings will open new avenues for novel treatments in which cell - specific enhancer sequences can be targeted to alter gene expression.

«New treatment targets cancers with particular genetic signature: Mutations in gene SETD2 make cancer cells vulnerable to drug inhibiting the protein WEE1.»

By synthesizing a nanoparticle that releases its siRNA cargo only after it enters targeted cells, Dr. Tariq M. Rana and colleagues showed in mice that they could deliver drugs that silenced the genes they wanted.

Whereas in the nematode experiment the researchers targeted nanoparticles to temperature - sensitive ion channels that naturally exist in the membranes of the worms» nerve cells, the scientists inserted the gene for a heat - activated ion channel called TRPV1 into the human and rat cells.

But so far, silencing genes using RNAi has not worked well in mammalian cells; although the approach has been successful in mouse embryos, double - stranded RNA shuts down synthesis of all proteins, not just the target gene, in other types of mammalian cells.

Given his training in developmental biology, Raman focused the team to seek a novel drug target on genes important to the development of model organisms — fruit flies (Drosophila) and yeast (Saccharomyces cerevisiae)-- rather than on oncogenes that transform a normal cell into a cancer cell.