Sentences with phrase «target genes sequenced»

An abundance of rare functional variants in 202 drug target genes sequenced in 14,002 people.

A local philanthropist underwrote purchase of a new Illumina MiSeq device that facilitates targeted gene sequencing.

Not exact matches

That's not to say gene editing is new (it isn't), but Crispr simplifies the process by using molecular scissors that can be precisely targeted to snip out aberrant regions of genetic code, which can then be replaced with the correct sequences.

In the study, whole - exome sequencing and a targeted analysis of 90 genes implicated in heart disease were applied to 419 unrelated SIDS cases.

Using a gene - sequencing technique known as whole - exome sequencing that targets only the DNA that encodes proteins, the researchers cataloged virtually every significant DNA variant in three families in which the condition is common.

In addition, they also used exome sequencing and RNAseq — sequencing directly targeted to the SHIP1 gene — to examine the structure of the SHIP1 gene at both the DNA and the RNA level.

The vaccine can be targeted to the chosen pathogen by using RNA molecules which share sequence identity with the pest's genes and prevents their expression.

Theoretically, it could be simpler and more targeted to use gene blueprints from sequencing data to build something that mimics microRNAs — binding to the same things the microRNA would — then embarking on a fecal transplant and ensuring it is safe.

First described in the 1990s — a discovery that led to the 2006 Nobel Prize — RNAi is a process by which organisms suppress the expression of target genes through the action of small RNA segments that bind to corresponding gene sequences.

In addition, it binds to the promoter sequences (dark blue) of specific target genes including mitochondrial fission process 1, MTFP1.

Unlike whole - genome sequencing, this assay targets a select set of genes or regions with known associations with lymphoid cancer, allowing for more rapid detection of a variety of mutations.

The gene - editing success appears to be largely due to one procedural change: The researchers introduced the editing system — the enzyme Cas9 and a guide RNA sequence that helps the editing machinery find its target — at the same time they injected the mutation - laden sperm into a healthy egg in the lab.

«Depending on the research questions, it may also be important to define the appropriate strategy to use, either whole - genome sequencing or just targeting some genomic regions with a gene baiting approach,» says Besnard.

Noncoding DNA includes stretches called enhancers, short sequences that help control a target gene.

The study adds to evidence that gene editing may need to be adapted to each patient's genome, to ensure there aren't variants in DNA sequence in or near the gene being targeted that would throw off the technology.

One general benefit of the RNAi approach, Zhang says, is that researchers can selectively target specific insect pests by targeting species - specific gene sequences; this avoids the blanket destruction of other insect species seen with many insecticides, he explains.

The gene that codes for this clotting protein has a very similar sequence across many plant species, and the researchers showed that the microRNA from dodder targets regions of the gene sequence that are the most highly conserved across plants.

«In addition, many of the high - frequency sequences span genes involved in the immune system, which is a frequent target of adaptive evolution.»

Choudhury used high - throughput sequencing technology to find the targets of DAZAP1 and decipher whether DAZAP1 would bind to the exons and introns of genes.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

Extensive research has already examined the function of microRNAs, a category of small evolutionarily conserved noncoding RNAs about 22 to 24 nucleotides in length that target protein - coding genes in a sequence - specific manner.

The Rutgers scientists show that the transcription activator protein functions by binding to a specific DNA sequence preceding the target gene and making adhesive, Velcro - like interactions with RNA polymerase that stabilize contacts by RNA polymerase with adjacent DNA sequences.

Furthermore, besides offering valuable insight into the function of this novel RNA type, the researchers also believe that the findings will open new avenues for novel treatments in which cell - specific enhancer sequences can be targeted to alter gene expression.

The brothers got the idea for the drug - gene interaction database after they repeatedly were asked whether lists of genes identified through cancer genome sequencing could be targeted with existing drugs.

Wall then isolated those fungi and sequenced the gene FKBP12 — the target of FK506 — to look for mutations that would confer drug resistance.

By using faster sequencing machines and targeting only one highly variable part of the rRNA gene, he and his team can sequence 200,000 pieces of DNA from a single liter.

The convergence of several factors explains the trend: cheaper genetic sequencing technologies, the discovery of new oncogenes (genes that can cause a normal cell to become cancerous), a new generation of computers and bioinformatics that can analyze vast amounts of data, and a multibillion - dollar effort by researchers inside and outside the pharma industry to develop targeted drugs and companion diagnostics for cancer.

A series of genetic analyses confirmed that the DNA target sequence had been altered in the transgenic plants, with either DNA insertions or deletions in both copies of the DFR - B gene (so - called bi-allellic mutants).

As reported on 30 August 2017 in Scientific Reports, a short DNA sequence in the Japanese morning glory DFR - B gene was selected as the target for the CRISPR / Cas9 system.

Canadian researchers conducted a pilot study with 20 newborns to determine the effectiveness of a targeted next - generation sequencing panel that included all 4813 genes currently known to be associated with rare diseases.

While circulating tumor DNA tests targeting a smaller set of cancer genes are already available for use in routine practice to guide care, by covering a much larger number of cancer genes, this high - intensity sequencing approach may enable development of future tests for early detection of cancer.

siRNAs are designed by taking short sequences of the gene to be targeted and converting them into double - stranded RNA.

«Understanding how Cas9 is able to locate specific 20 - base - pair target sequences within genomes that are millions to billions of base pairs long may enable improvements to gene targeting and genome editing efforts in bacteria and other types of cells,» says Doudna who holds joint appointments with Berkeley Lab's Physical Biosciences Division and UC Berkeley's Department of Molecular and Cell Biology and Department of Chemistry, and is also an investigator with the Howard Hughes Medical Institute (HHMI).

Moreover, the ability to easily program sequence - specific DNA targeting and cleavage by CRISPR - Cas components, as demonstrated for Cas9 and Cpf1, allows for the application of CRISPR - Cas components as highly effective tools for genetic engineering and gene regulation in a wide range of eukaryotes and prokaryotes.

He says HGS was getting «diminishing returns» from its investment in TIGR since Venter had steered his outfit into sequencing organisms of little medical importance, and into human genome sequencing, also of limited value for a company like HGS that is interested in genes as drug targets (not untranslated DNA that makes up most of the genome).

«These sequences then become the targets for a far narrower set of follow - up gene function studies in F. graminearum by reverse genetic experimentation to pinpoint the genes essential for the disease - causing abilities of this pathogen.»

Targeted gene therapies, however, had to wait for (1) the identification of the genes to target, (2) the cloning and / or sequencing of the relevant genes and in some cases, the specific disease - causing variant, (3) a full understanding of the normal gene function and regulation, and (4) the development of efficient ways to deliver genes to the relevant tissues at therapeutic levels.

One ZFN pair was used to target both the human and rhesus macaque CXCR4 genes since the 24 bp target sequences are identical.

The team designed the PshRNA agent selectively targeting the common sequence of human and mouse (pro) renin receptor genes.

If the beetle does evolve to resist an RNA molecule, he says, geneticists could easily launch a new assault: just «slide the sequence over» by a few letters or target several genes at once.

The advent of molecular cloning, DNA sequencing and the many tools of molecular genetics and cell biology has given us sufficient knowledge of the basis for disease and the genes to target, but what has limited the application of gene therapy has been efficient gene delivery systems.

The ZFNs targeting either the cxcr4 or ccr5 genes were linked via a 2A peptide sequence and cloned into the pAdEasy - 1 / F35 vector under control of the CMV TetO promoter, and the Ad5 / F35 virus for each construct was generated using TREx 293T cells as described [33].

Immunizing drives could block the spread of unwanted gene drives by preemptively altering target sequences.

Sarah Weckhuysen and colleagues report a study based on targeted sequencing of the genes encoding the components of the GATOR1 (DEPDC5, NPRL2, and NPRL3) and GATOR2 (MIOS, SEC13, SEH1L, WDR24, and WDR59) complex in European probands with focal epilepsy with or without focal cortical dysplasia.

His work centers on developing general, rational approaches to design precision medicines from genome sequences by targeting the RNA product of genes.

Molecular studies of Hox - regulated target genes suggest that these changes occur through accumulation of mutations in regulatory DNA sequences.

We helped with the analysis of targeted 3730 sequencing of some candidate genes in a warfarin patient cohort, and uncovered another gene, (CALU, encoding calumenin) in which variants influenced warfarin dose.

The advancement of the Cas9 - based platform for screening and validation will help further the development of new therapeutic products, and Caribou's CRISPR - Cas9 technology can utilize guide RNAs specific for unique sequences and target a gene at numerous sites and therefore provide enhanced specificity.

Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes.

A Next - Generation Framework: Deciding On The Role Of Costs In The Clinical Use Of Targeted Gene Panels, Exome And Genome Sequencing.