Sentences with phrase «target loci»

We exploited a new method, differential viral accessibility (DIVA), to show that loss of MORC2 results in chromatin decompaction at these target loci, which is concomitant with a loss of H3K9me3 deposition and transcriptional derepression.

Mechanistically, SMAD3 interacts with reprogramming factors and co-activators and co-occupies OCT4 target loci during reprogramming.

To account for the placement of gaps from one genome into another, we adjusted the genomic coordinates at the target loci, resulting in a synthetic genome for both species (Methods).

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Gesicles are made by transfecting a 293T - based producer cell line with a vector containing an sgRNA for your target locus and our gesicle packaging mix, which contains everything you need in an optimized format.

USA 89, 5128 - 5132) and the length of homologous sequence of the targeting vector and the location of the target locus.

A final ligation reaction correctly assembles the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and marker sequence into the targeted loci.

MSTN / PrP double mutations in cell colony make the target locus resistant to Van91I and Bsu36I treatment (arrowhead).

Site - specific mutations will make the target locus resistant to the restriction enzyme treatment (uncut), which can be detected by restriction fragment length polymorphism (RFLP) assay.

MSTN mutations in cell colony make the target locus resistant to Van91I treatment (arrowhead).

Not exact matches

Chromatin immunoprecipitation sequencing analysis revealed enrichment of H3K27me3 at specific loci in KDM6A - null cells, including PRC2 / EZH2 and their downstream targets.

Cas9 creates double - strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide.

In mice heterozygous for tau - lacZ targeted to the melanopsin gene locus, β - galactosidase — positive RGC axons projected to the SCN and other brain nuclei involved in circadian photoentrainment or the pupillary light reflex.

In the presence of an input, ssDNAs (orange curved lines) are produced from a plasmid - borne cassette (gray circles) and recombined into specific genomic loci (orange circles) that are targeted on the basis of sequence homology.

The dual - effect risk loci also include the region covering the gene FABP4, which is already being investigated for its potential as a diabetes and heart - disease drug target.

These intracellularly expressed ssDNAs are targeted into specific genomic loci where they are recombined and converted into permanent memory.

However, our analysis of a more diverse sample set that includes village dogs confirms only 18 of these loci as putative domestication targets, the remainder are likely associated with breed formation (Supplementary Table 19 and Supplementary Note 14).

Feralisation targets different genomic loci to domestication in the chicken.

Additionally, consider testing multiple gRNAs targeted to the same locus to ensure their effects are specific to that locus.

This showed all markers as C57BL / 6J allele - type - the only exceptions being the closest markers flanking the targeted Gad2 locus were segregating with the Gad2 - IRES - Cre allele (i.e., heterozygous for C57BL / 6J; 129 allele - type).

A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt - flanked neo cassette into the 3» untranslated region (after the translational termination site) of the glutamic acid decarboxylase 2 locus (Gad2) on chromosome 2.

our stroke research, we will soon be able to find a gene within the type 2 diabetes locus we have identified and use this gene to identify powerful new drug targets.»

Image: Punctate loci obtained by expression and chloroplast targeting of multiple carboxysomal proteins (Red = chlorophyll autofluorescence; Green = YFP label on a carboxysomal protein)

Identification of Functional Variants for Cleft Lip with or without Cleft Palate in or near PAX7, FGFR2, and NOG by Targeted Sequencing of GWAS Loci The American Journal of Human Genetics DOI: 10.1016 / j.ajhg.2015.01.004

Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus.

Targeted Gene Addition to a Safe Harbor locus in human CD34 + Hematopoietic Stem Cells for Correction of X-linked Chronic Granulomatous Disease.

In other words, modification of chromatin arises due to cellular signaling to particular genome loci and does not appear to be propagated independently of genome targeting.

Furthermore, because the TRG locus is rearranged before the TRB locus and these clonal rearrangements persist, even in alpha - beta T cells, laboratories testing for IGH, IGK and the TRG together are likely to detect at least one clonal biomarker target in the vast majority of hematologic malignancies.

The Cheng lab develops technologies based on artificial DNA and RNA binding proteins and applies them to sense or induce sequence or epigenetic changes at target genomic loci or target transcripts.

Note: For a more thorough explanation of the locus and the targeted sequencing strategy, please refer to (Miller J.E., 2013).

Protein - altering variants within 500 kb of 22 «index SNPs» uncovered in targeted sequencing of GWAS loci

Genomic DNA digested with EcoRV was hybridised with either probe 1 to reveal WT (11.3 kb) or targeted (9.3 kb) bands, or probe 2 to produce a 9.3 kb band representing a single integration only in the Hex locus.

Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases.

Dr. Radislav Sedlacek and his team from the Institute of Molecular Genetics has recently published new paper on gene targeting of Rosa26 locus using TALENs.

Following electroporation into R26 BirA cells, a cell line that expresses bacterial BirA ligase from the ROSA26 locus, hygromycin resistant clones (200 µg / ml) were expanded for Southern analysis to identify correct targeting events.

In some species copy number variation (CNV) of target site loci (e.g. the Ace - 1 target site of carbamate insecticides) or detoxification genes has been implicated in the resistance phenotype.

Targeted sequencing, in which only a selected set of genomic loci are sequenced, enables a much higher coverage of each target than what is obtained using whole genome or exome sequencing.

Insecticide resistance is typically associated with alterations to the insecticidal target - site or with gene expression variation at loci involved in insecticide detoxification.

Despite this, we were able to pinpoint two RNA pseudouridine synthases, which were each strongly linked to a separate set of loci across the chromosome, representing biologically plausible targets of co-selection.

This review focuses on a distinctive group of type I RM loci that, through phase variation, can modify their methylation target specificity and can thereby switch bacteria between alternative patterns of DNA methylation.

The premise involves a shapeshifting monster that stalks its targets until they have sex, passing on the curse to someone else, and though a generation ago, this would seem like a fairly clear - cut AIDS metaphor, Mitchell (The Myth Of The American Sleepover) makes it at once more complicated (for instance, after killing a target, the titular It begins stalking the previous one again) and more primal, a locus for all kinds of sexual and social fears that horror movies tend to express more clearly than any other genre.

Doing a genetic test and subsequently eliminating an individual from the breeding population may not be the best strategy, as by targeting a particular allele at one genetic locus for removal from the gene pool of a particular breed, breeders may in fact increase allele frequency of genetic variants on alternative haplotypes at the same, or a different locus, that are recessively deleterious.