Sentences with phrase «target locus»

The Morris lab has since determined the mechanistic underpinnings of non-coding RNA mediated regulation of gene transcription in human cells, with evidence suggesting a role for longer forms of non-coding RNAs as endogenous effectors involved in epigenetically remodelling target loci in human cells.

When coexpressed with a recombinase, these ssDNAs address specific target loci on the basis of sequence homology and introduce precise mutations into genomic DNA, thus converting transient cellular signals into genomically encoded memory.

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

To account for the placement of gaps from one genome into another, we adjusted the genomic coordinates at the target loci, resulting in a synthetic genome for both species (Methods).

Gesicles are made by transfecting a 293T - based producer cell line with a vector containing an sgRNA for your target locus and our gesicle packaging mix, which contains everything you need in an optimized format.

USA 89, 5128 - 5132) and the length of homologous sequence of the targeting vector and the location of the target locus.

A final ligation reaction correctly assembles the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and marker sequence into the targeted loci.

MSTN / PrP double mutations in cell colony make the target locus resistant to Van91I and Bsu36I treatment (arrowhead).

To facilitate mutation analysis, a restriction enzyme recognition site was incorporated in each target locus (Figure 1A).

MSTN mutations in cloned goat make the target locus resistant to Van91I treatment (arrowhead).

Site - specific mutations will make the target locus resistant to the restriction enzyme treatment (uncut), which can be detected by restriction fragment length polymorphism (RFLP) assay.

MSTN mutations in cell colony make the target locus resistant to Van91I treatment (arrowhead).