Sentences with phrase «targeting vector»

This publicly available BAC resource can be used for the rapid construction of targeting vectors via recombineering.

Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129 - derived ES cell lines.

SapTrap offers scientists a convenient one tube assembly reaction to generate high - throughput targeting vectors.

Targeting vectors as part of a preventive approach to controlling mosquito - borne human diseases has been critically important for decades, but has received much less attention in companion animal disease prevention.

Or some kind of targeted vector that can transfect muscle tissue or some other tissue type alone?

from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA

Other investigators who had been working to develop high efficiency gene targeting approaches improved on this finding with the addition of selectable markers that are only expressed when the targeting vector is inserted downstream of a functional promoter (gene traps).

In that year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA.

A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt - flanked neo cassette into the 3» untranslated region (after the translational termination site) of the glutamic acid decarboxylase 2 locus (Gad2) on chromosome 2.

USA 89, 5128 - 5132) and the length of homologous sequence of the targeting vector and the location of the target locus.

A targeting vector was designed to contain the Enhanced Yellow Fluorescent Protein gene (from the pEYFP - N1 plasmid, Clonetech) downstream of a loxP - flanked stop sequence (neomycin resistance gene and a trimer of the SV40 polyadenylation sequence).

The targeting vector was integrated into the fly genome to generate a donor line.

A final ligation reaction correctly assembles the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and marker sequence into the targeted loci.

More specifically, the Euclidean distance, d, between the target vector [ut, vt] and the (rotated) movement vector [ui, vi] had to be less than 0.3 mm.

This entire cassette was fused in frame with the ATG of Hex in the targeting vector.

The reporter and a LoxP flanked selection cassette was inserted downstream of a tagged Hex cDNA to generate the Hex - IRES - Venus (HV)(Fig. 1A) targeting vector.

We constructed a targeting vector by deleting a ~ 2.6 kb region containing exons 1 and 2 and ~ 1 kb of the sequence upstream of exon1 and used the vector to generate the knockout mouse.

Ellappan Babu, Yangzom Bhutia and Sabarish Ramachandran performed most of the experiments; Jaya Gnanaprakasam did the ELISA for cytokines; Puttur Prasad and Muthusamy Thangaraju were responsible for the design of the targeting vector used in the generation of the knockout mouse and also for the analysis of plasma amino acids; Vadivel Ganapathy was responsible for the design of the study, interpretation of the data, and writing of the manuscript.

ES cells and targeting vectors can be ordered directly from the IKMC repositories.

A, Targeting vector was designed to disrupt the RhoH gene by homologous recombination.