Sentences with phrase «targets than genes»

Not exact matches

In our tests, the Gene Knockout Kit gave us greater than 80 percent knock - out rates for seven targets,» shares Shondra Miller, Ph.D., Director, Center for Advanced Genome Engineering at St. Jude Children's Research Hospital, on Synthego's web site.

«Those carrying the target gene variant slept, on average, for five hours, which was one hour shorter than their twins without the gene.

Since it is proteins that do the reprogramming, rather than the genes that make them, the researchers reasoned that they simply needed to get enough of the proteins into the target cells.

«The state of the art right now is targeting two or three genes simultaneously and then looking at the effects, but we think that perhaps the gene sets that need to be modulated to address some of these diseases are actually broader than that,» says Lu, who is the senior author of the study.

Parkinson contends that targeted gene replacement produces cells having specific genetic alterations far more effectively than the traditional technique for making transgenic animals, which entails injecting DNA into cell nuclei.

It is typically less expensive to get preselected information about the 20,000 or so genes that make up a person's exome — the section of the genome that provides instructions for making proteins — than to perform a more precision - oriented test that targets a single gene.

The former target, say, using gene editing techniques to inactivate HIV receptors and achieve resistance of blood cells to the virus (which Sangamo BioSciences is working on in clincial trials) is different than helping parents who both carry genes for Huntington's Disease to have a child that is free of the disease (a change to the genome that would be passed on to future generations and would likely not be very commonly needed).

«The genes which these miRNAs regulate also had increased levels, indicating that these gene expression, indicating that these gene products were likely targeted for storage and for possible future use within the brain cell, rather than for destruction.

The same process has been studied as a potential genetic therapy for more than a decade, because you can target any disease gene with matching dsRNA.

Rather than target a tumor - suppressor gene directly, Ideker and team took the approach of identifying genetic interactions between a tumor suppressor gene and another gene, such that simultaneous disruption of both genes selectively kills cancer cells.

In gene - baiting, scientists use targeted PCR primers to amplify specific genes during the library preparation step, rather than amplifying all of the DNA fragments in the sample.

«ALK now becomes the second abnormal gene that we are able to successfully target in lung cancer with drugs other than chemotherapy.»

Beginning in 2012, researchers learned to modify this mechanism to precisely target unwanted genes rather than viruses.

Because the vaccine causes the body to mount an immune response directed against a unique tumor, the therapy is much more effective than gene - targeted or more general chemotherapy alone.

It includes more than 14,000 drug - gene interactions involving 2,600 genes and 6,300 drugs that target those genes.

Importantly, mutations in the BRAF gene that are seen in more than half of advanced melanomas were absent in mucosal melanoma, explaining the ineffectiveness of BRAF - targeted treatments like vemurafenib.

In fact, the researchers point out that phase 1 clinical trials are already underway for compounds targeting this gene in other cancers, meaning that the time needed to apply a similar strategy to mucosal melanoma could be dramatically shorter than if they had to start from scratch.

Given his training in developmental biology, Raman focused the team to seek a novel drug target on genes important to the development of model organisms — fruit flies (Drosophila) and yeast (Saccharomyces cerevisiae)-- rather than on oncogenes that transform a normal cell into a cancer cell.

One of the gene areas identified suggests that cells within the lining of the uterus play a larger - than - expected role in the length of pregnancy, which in turn provides a new target for medications to help prevent preterm birth.

More than 20 of the overexpressed genes can be targeted with existing drugs to muffle them, suggesting patients could be tested and matched to these drugs, says TCGA investigator Douglas Levine of Memorial Sloan - Kettering Cancer Center in New York City.

Since these proteins serve as «master regulators» for controlling whole gene programs, drugs against these targets could have broader effects than traditional ones that block single enzymes.»

from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA

«It is therefore understandable that natural selection may have favored the relative targeting of MMR to genes rather than non-genic regions.

In that year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA.

We are investigating the genes that are the target of this pathway, how it influences the cells of growing worms, the size differences between the sexes (hermaphrodite worms are larger than males), and the way in which it mediates signals from the environment.

The study also showed that WGS identified more clinically actionable mutations than the current standard of examining a limited subset of genes, known as a targeted panel.

Dr. Richard Pazdur, cancer drugs chief at the Food and Drug Administration, warned that although everyone hopes that targeting drugs to gene mutations will improve survival, «this may be far more complex than we realize.»

Homozygous lethality of the XPCS allele is likely due to reduced levels of expression of this essential protein as a result of gene targeting (Figure 1A) rather than to the mutation itself.

What these groups are attempting basically is «gene silencing», with a twist - it's aimed directly at the DNA, rather than targeting a «message molecule» called RNA.

So, a drug that targets CAG tracts could switch off those genes too, which could end up doing more harm than good.

Although CRISPR is incredibly useful for generating mutations by NHEJ and generating small mutations with HDR, when it comes to larger scale genome editing, such as replacement of a mouse gene with its human ortholog (greater than 5 kb), it remains to be seen whether CRISPR is as robust as conventional gene targeting.

PrediXcan has the potential to identify gene targets for therapeutic applications faster and with greater accuracy than traditional methods.

The gene produces an enzyme (diacylglycerol kinase eta) that functions at a point closer to the root of the lithium - sensitive pathway than does the protein that lithium is thought to target.

The majority of gene - targeting experiments in mice are performed in 129Sv - derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains.

The CRISPR / Cas technology applied to mouse genetic engineering could quickly advance scientific understanding of disease mechanisms by allowing researchers to ask complex questions and find answers much faster than with traditional gene targeting approaches.

Given the tailored inhibition of selected genes and the added precision brought by targeted delivery systems, RNAi - based therapies are thought to carry lower risk of failure than traditional approaches as the biological effects are more predictable.

They discovered that CRISPRi was much more efficient than CRISPR - Cas9: in more than 95 % of the cells created using CRISPRi, the target gene was silenced, compared with only 60 - 70 % of cells grown from CRISPR - Cas9.

But rather than changes in doublesex itself, these studies revealed changes in downstream targets of dsx, via changes to specific DNA sequences to which DSX protein binds in the cis - regulatory regions of the bric - a-brac and desatF genes and affecting sex differences in abdominal pigmentation and pheromone production.

In research that began more than a decade ago, Biswal found GAUT4 expressed in poplar and then targeted the gene in both poplar and switchgrass.

Comparing this approach to the classic CRISPR / Cas9 system designed to cleave the DNA, the Gladstone team found that CRISPRi is actually more effective, silencing the target gene in more than 95 percent of cells, compared with the 60 percent to 70 percent efficiency of CRISPR / Cas9.

A variation of the gene - editing technique can more precisely and efficiently downregulate the expression of target genes than traditional CRISPR / Cas9.

Prolonged in vitro culture during gene targeting found D4 to be more susceptible to differentiation than C2.

CRISPR is now so well known that Google finally stopped suggesting I may be looking for «crisps» instead, but the real - world applications are not so well worked out yet, and there are various issues around CRISPR, including off - target effects, and also the fact that deleting genes is much easier than replacing them with something else.

A subset of these miRNA knockout lines are «cluster» knockouts in which more than one gene has been targeted.

To date, only 100 or so of the more than 20,000 genes in the human genome have been developed as targets for FDA - approved drugs.

It is always possible that a vector will introduce the gene into a cell other than that for which it is supposed to be targeted (e.g., a spermatocytic cell) or that through a secondary mechanism target cells that have taken up the new gene will through some independent natural process (such as transfection) transfer the gene to a germline cell.