Sentences with phrase «then cell sorting»

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If the divine is now used to give the view a supposedly greater philosophical coherence, then I inevitably reach the sort of conclusion implied by Hartshorne's bodily cells with their «little experiences or feelings.»

We anticipate some sort of growth toward increased complexity: increasingly larger organic macromolecules, then the convergence of many macromolecules to constitute a simple living system, either as a cell with its protective wall and vital nucleus or as some functional analogue, then the convergence of many cells to form larger organisms.

This part of the cell then becomes an intensive production shop of sorts, generating the proteins needed for processing the food.

Fluorescence - activated sorting is then used to identify and retrieve from this mix only those cells that display strong reporter gene expression, which represent the cells with the most active enhancers.

This complex biological machine gathers the chromosomes together and sorts them at the time of cell division, then sends them to the opposite poles of the daughter cells in a process called chromosome segregation.

CRH, in turn, triggered production of the cell surface protein FasL; trophoblasts expressing FasL then form a protective shield of sorts around the embryo by forcing any attacking immune cells to self - destruct.

They first devised a nanoscale plastic scaffold and then seeded it with common muscle precursor cells called myoblasts, along with endothelial cells of the sort found on the inside of blood vessels.

To function, most of the newly - produced proteins first need to be sorted and then transported within and outside of the cell.

So if you think about the egg as sort of a uniform ball, you put a messenger RNA on one end of the egg, and when you cut up the ball, then only the cells at that end get that information.

After the sorting, we performed whole genome amplification on individual aggregates then sequenced the 16S rRNA marker genes to identify cells comprising the aggregate.

So, for example, at the University of California in San Francisco they are trying to engineer E. coli so that it can detect cancer cells, it can invade tumors, and then once it's inside they can release toxins; and so they are putting in all sorts of genes from other bacteria to assemble this, you know, this sort of synthetic E. coli that could become basically a cancer torpedo.

They feed on the bottom of the food chain — on single - celled plankton, which larger fish can not eat — and then they become prey for all sorts of upper - level predators like tuna, sea bass and halibut as well as seabirds and marine mammals.

The group was then able to sort the cells using markers expressed on the cell surface, to help pull out successfully edited cells for research, and eventually for therapeutic use.

300 μl of ice - cold 1 × binding buffer were then added to each sample and the samples were analyzed by fluorescence - activated cell sorting (FACS) within one hour for maximal signal.

Current efforts, mostly conducted in animal models, involve attempting to derive and aggregate embryonic stem cells, exposing them to such factors as acetic acid, allowing them to differentiate, or specialize, and then sorting through these cells to extract the cell types of interest.

Single cells from this sorted population were then seeded into each well of a 96 - well plate by limiting dilution.

We delve into very fundamental problems such as «how does a malaria red blood cell attach itself to a blood vessel» or «how does binding of a ligand to a cell surface receptor or contact of a crystalline surface with the plasma membrane drive lipid sorting and how will this lead to signalling» but then immediately apply it to a practical problem such as «how does contact of uric acid crystals with dendritic cells cause gout in affected joints and how can we prevent this occurrence?»

We were then able to sort through both sets of sequences and detect mutant cells — even when they made up as little one in every thousand cells.

We can then take proteins of interest, change them in some specific way, and then put them back into a cell to create a sort of modified synapse.

The B cell depleted fraction was then surface stained for lineage markers (CD3, CD19, CD14), CD127 and cells were sorted (Lin - CD127 +).

To overcome this problem, the researchers utilized a method that involved dissolving the cell membranes in order to create a sort of «brain soup» so that they can then count the number of cell nuclei in a sample.

To do this, it randomly sorts chromosomes from both sets in one cell division and then reduces them by half in another.

After secondary antibody binding the cells were washed three times with ice - cold PBS - G and then resuspended in 2 ml of ice - cold PBS - G, passed again through a 40 micrometer filter and then immediately analyzed and sorted on a FACSAria cell sorter (BD Biosciences, San Jose, CA, USA, http://www.bdbiosciences.com) with blue laser excitation (488 nm).

Manipulating genes and then sorting through many cells to find the desired alterations wasn't possible with these cells because scientists couldn't grow them in the culture dishes where such maneuvers would take place.

Dave Asprey: What we did is I had my stem cells taken out, and my wife, Dr. Lanna, had her stem cells taken out, actually extracted from our own fat, and then re-injected at various parts of the body, all sorts of injury sites.

At first I thought we were talking about something like what I saw, like sort of a tablet maybe or notebook or netpad or whatever, then I thought we were talking about a smart - phone - cell - phone, now I am not sure what we are talking about.

Making it to the end of a dungeon lets you interact with a shopkeeper of sorts, spending any collected cells on permanent upgrades that persist between playthroughs or depositing blueprints for new gear that then becomes unlocked and upgradable.

There's all these geometric pieces that evoke prison cells or biological cells, but then you have this sketch for a hypothetical cinema inside a cavern — this sort of room to dream inside a spelunker's place of self - imposed confinement.

The solid oxide fuel cell under the coals then converts carbon and hydrogen into electricity, and is described as a sort of reverse photosynthesis:

And then comes the astonishingly dramatic Cell Block Tango from the movie Chicago, in which all the potential aggression in an argument between lovers is emphasized to the ultimate (and which, in a nifty sort of wrap - up to this fillip, brings us back to law again).